Hostname: page-component-8448b6f56d-mp689 Total loading time: 0 Render date: 2024-04-20T10:19:28.776Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular identification and seasonal infections of species of Fasciola in ruminants from two provinces in China

Published online by Cambridge University Press:  30 June 2015

W. Yuan ,
J.-M. Liu ,
K. Lu ,
H. Li ,
M.-M. Duan ,
J.-T. Feng ,
Y. Hong ,
Y.-P. Liu ,
Y. Zhou  and
L.-B. Tong
...Show all authors
W. Yuan
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
J.-M. Liu*
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
K. Lu
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
H. Li
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
M.-M. Duan
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
J.-T. Feng
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
Y. Hong
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
Y.-P. Liu
Affiliation:
Anhui Center for Animal Disease Control and Prevention, Hefei230000, P.R. China
Y. Zhou
Affiliation:
Hubei Center for Animal Disease Control and Prevention, Wuhan430000, P.R. China
L.-B. Tong
Affiliation:
Wangjiang County Center for Animal Disease Control and Prevention, Anqing246000, P.R. China
J. Lu
Affiliation:
Gongan County Center for Animal Disease Control and Prevention, Jingzhou434000, P.R. China
C.-G. Zhu
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
Y.-M. Jin
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
G.-F. Cheng
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China
J.-J. Lin
Affiliation:
National Laboratory of Animal Schistosomiasis Control, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai200241, P.R. China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou225009, P.R. China
*
*Fax: +86 21 34293619 E-mail: jimyliu@shvri.ac.cn
Get access Rights & Permissions [Opens in a new window]

Abstract

We determined the prevalence and seasonality of infections by Fasciola of goats and bovine species (cattle and water buffalo) in Hubei and Anhui provinces of China. Faecal samples were collected at 2- to 3-month intervals from 200 goats in Hubei province and from 152 bovine species in Anhui province. All faecal samples were examined for the presence of parasites. We determined the nucleotide sequences of the first and second internal transcribed spacers (ITS-1 and ITS-2) of the nuclear ribosomal DNA (rDNA) of 39 Fasciola worms from Anhui province. The prevalence of Fasciola infection in goats ranged between 3.5 and 37.0%, with mean eggs per gram (EPG) ranging between 29.0 and 166.0. Prevalence and EPG exhibited downward trends over time with significant differences. The prevalence of Fasciola infection in cattle ranged between 13.3 and 46.2% (mean EPG, 36.4–100.0), and that of water buffalo ranged between 10.3 and 35.4% (mean EPG, 25.0–89.6), with a higher prevalence of infection and EPG from June to October compared with December to March. Analysis of ITS-1 and ITS-2 sequences revealed that F. hepatica and F. gigantica were present in all bovine species of Anhui province and that F. gigantica mainly infected water buffalo. This is the first demonstration of Fasciola infection in Hubei province and detection of F. hepatica and F. gigantica in Anhui province. The present study of Hubei province shows that mass treatment of livestock with closantel sodium injections in April and August/September controlled Fasciola infection effectively.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 3 , May 2016 , pp. 359 - 363
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alasaad, S., Granados, J.E., Cano-Manuel, F.J., Meana, A., Zhu, X.Q. & Pérez, J.M. (2008) Epidemiology of fasciolosis affecting Iberian ibex (Capra pyrenaica) in southern Spain. Parasitology Research 102, 751–755.Google Scholar
Ali, H., Ai, L., Song, H.Q., Ali, S., Lin, R.Q., Seyni, B., Issa, G. & Zhu, X.Q. (2008) Genetic characterization of Fasciola samples from different host species and geographical localities revealed the existence of F. hepatica and F. gigantica in Niger. Parasitology Research 102, 1021–1024.CrossRefGoogle Scholar
Chen, J.X., Chen, M.X., Ai, L., Xu, X.N., Jiao, J.M., Zhu, T.J., Su, H.Y., Zhang, W., Luo, J.J., Guo, Y.H., Lv, S. & Zhou, X.N. (2013) An outbreak of human fascioliasis gigantica in southwest China. PLoS One 8, e71520.Google Scholar
Chen, S.B., Hasi, S.R. & Amu, G.L. (2013) Comparative analysis and digital description of the microscopic characteristics of nine kinds of helminth eggs of ruminants. Acta Zoologica Sinica 44, 931–936 (in Chinese).Google Scholar
Dar, Y., Amer, S., Mercier, A., Courtioux, B. & Dreyfuss, G. (2012) Molecular identification of Fasciola sp. (Digenea: Fasciolidae) in Egypt. Parasite 19, 177–182.Google Scholar
Dorny, P., Stoliaroff, V., Charlier, J., Meas, S., Sorn, S., Chea, B., Holl, D., Van Aken, D. & Vercuysse, J. (2011) Infection with gastrointestinal nematodes Fasciola and Paramphistomum in cattle in Cambodia and their association with mobidity parameters. Veterinary Parasitology 175, 293–299.Google Scholar
Gu, Y., Shen, Y., Ferre, I., Kraemer, F. & Gonzaloz, J. (1998) Investigation and analysis of Fasciola hepatica infection of buffalo in Jiangsu and Anhui Provinces. Chinese Journal for Veterinary Science and Technology 28, 14–16 (in Chinese).Google Scholar
Huang, D.M., Lin, X.M., Zhou, K.F., Chen, Q.Q. & Tang, C.T. (1997) Faecal examination of Fasciola spp. of bovines in Jiumu Town, Fujian Province. Journal of Tsinghua University (Science and Technology) 37, 47–49 (in Chinese).Google Scholar
Itagaki, T., Kikawa, M., Sakaguchi, K., Shimo, J., Terasaki, K., Shibahara, T. & Fukuda, K. (2005a) Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA. Parasitology 131, 679–685.Google Scholar
Itagaki, T., Kikawa, M., Terasaki, K., Shibahara, T. & Fukuda, K. (2005b) Molecular characterization of parthenogenic Fasciola sp. in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene. Journal of Veterinary Medical Science 67, 1115–1118.Google Scholar
Khallaayoune, K.I., Stromberg, B.E., Dakkak, A. & Malone, J.B. (1991) Seasonal dynamics of Fasciola hepatica burdens in grazing Timahdit sheep in Morocco. International Journal for Parasitology 21, 307–314.Google Scholar
Liao, D.J. (1996) Study on the new egg count technique for M. hirudinaceus and A. suum . Veterinary Parasitology 61, 113–117.Google Scholar
Lin, R.Q., Dong, S.J. & Xue, C.X. (2004) DNA polymorphism of the first internal transcribed spacer of Fasciola spp. Chinese Journal for Veterinary Science and Technology 34, 8–12 (in Chinese).Google Scholar
Lin, R.Q., Dong, S.J., Nie, K., Wang, C.R., Li, A.X., Song, H.Q., Huang, W.Y. & Zhu, X.Q. (2007) Sequence analysis of the first internal transcribed spacer of rDNA supports the existence of an intermediate Fasciola between F. hepatica and F. gigantica in Mainland China. Parasitology Research 101, 813–817.CrossRefGoogle Scholar
Lin, Y.G., Sun, Y.L., Chen, C.R. & Chen, D.X. (1974) The developmental history of Fasciola gigantica and epidemiology study of Fasciola spp. Acta Zoologica Sinica 20, 378–390 (in Chinese).Google Scholar
Luton, K., Waller, D. & Blair, D. (1992) Comparison of ribosomal internal transcribed spacers from two congeneric species of flukes (Platyhelminthes: Trematoda: Digenea). Molecular and Biochemical Parasitology 56, 323–327.Google Scholar
Nginyi, J.M., Duncan, J.L., Mellor, D.J., Stear, L.J., Wanyangu, S.W., Bain, R.K. & Gatongi, P.M. (2001) Epidemiology of parasitic gastrointestinal nematode infections of ruminants on smallholder farms in central Kenya. Research in Veterinary Science 70, 33–39.Google Scholar
Spithill, T.W. & Dalton, J.P. (1998) Progress in development of liver fluke vaccines. Parasitology Today 14, 224–228.Google Scholar
Wang, D.Y., Zhang, W.Y. & Huang, W.Y. (2006) Investigation on the infection of Fasciola spp. of bovines in Guangxi Province. Veterinary Animal Husbandry in Guangxi 22, 200–202 (in Chinese).Google Scholar
Xie, Y.J., Liu, W., Yang, J.H., Li, X.H. & Tan, X.Q. (2005) Investigation on the infection of Fasciola hepatica of bovines in Yueyang Hunan Province. Chinese Journal of Veterinary Parasitology 13, 20–21 (in Chinese).Google Scholar
Xu, L.Q., Yu, S.H. & Xu, S.M. (1999) Distribution and pathogenic impact of human parasites in China. 928 pp. Beijing, Beijing People's Medical Publishing House.Google Scholar
Zumaquero-Ríos, J.L., Sarracent-Pérez, J., Rojas-García, R., Rojas-Rivero, L., Martínez-Tovilla, Y., Valero, M.A. & Mas-coma, S. (2013) Fascioliasis and intestinal parasitoses affecting school children in Atlixco, Puebla State, Mexico: epidemiology and treatment with nitazoxanide. PLoS Neglected Tropical Diseases 7, e2553.Google Scholar