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Protective effect of dietary long-chain n-3 polyunsaturated fatty acids on bone loss in gonad-intact middle-aged male rats

Published online by Cambridge University Press:  08 March 2007

Chwan-Li Shen ,
James K. Yeh ,
Jahan Rasty ,
Yong Li  and
Bruce A. Watkins
Chwan-Li Shen*
Affiliation:
Texas Tech University Health Sciences CenterDepartment of Pathology, Lubbock, TXUSA
James K. Yeh
Affiliation:
2Winthrop-University HospitalBone Metabolism Laboratory, Mineola, NYUSA
Jahan Rasty
Affiliation:
3Texas Tech UniversityDepartment of Mechanical Engineering, Lubbock, TXUSA
Yong Li
Affiliation:
4Purdue UniversityCenter for Enhancing Foods to Protect Health, Lipid Chemistry and Molecular Biology Laboratory, West Lafayette, INUSA
Bruce A. Watkins
Affiliation:
4Purdue UniversityCenter for Enhancing Foods to Protect Health, Lipid Chemistry and Molecular Biology Laboratory, West Lafayette, INUSA
*
*Corresponding author: fax +1 21 806 743 2766, email Leslie.Shen@ttuhsc.edu
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Abstract

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This study evaluated the effect of a fat blend containing long-chain (LC) n-3 PUFA on bone mineral density (BMD) and bone metabolism in gonad-intact middle-aged male rats (12 months old, n 28). Seven rats were killed on day 0 of dietary intervention to determine the baseline BMD. The remaining rats (seven per group) were fed a diet with one of the following dietary lipid treatments (g/kg diet): 167g safflower oil+33g menhaden oil (N6+N3 diet, control), 200g safflower oil (N6 diet, almost devoid of LC n-3 PUFA), or 190g menhaden oil+10g corn oil (N3 diet, rich in LC n-3 PUFA) for 20 weeks. After 20 weeks, all dietary treatment groups had a lower BMD compared with the baseline reference. However, rats fed the N3 diet had the highest bone mineral content and cortical+subcortical BMD compared with those fed the N6 and control N6+N3 diet. Compared with the control (N6+N3) group, rats fed the N3 diet had higher values for serum insulin-like growth factor-I, parathyroid hormone, 1, 25-(OH)2 vitamin D3 and bone-specific alkaline phosphatase activity, but lower bone NO production and urinary Ca, whereas rats fed the N6 diet had higher bone prostaglandin E2 production and serum pyridinoline. These findings indicate a protective action of LC n-3 PUFA on ageing-induced bone loss in gonad-intact middle-aged male rats through a modulation of local factors and systemic calcitrophic hormones.

Keywords

Menhaden oiln-3 PUFABone metabolismGonad-intact middle-aged male rats
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 3 , March 2006 , pp. 462 - 468
Copyright
Copyright © The Nutrition Society 2006

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