Hostname: page-component-8448b6f56d-c47g7 Total loading time: 0 Render date: 2024-04-18T15:04:07.579Z Has data issue: false hasContentIssue false
  • English
  • Français

Monitoring the escape of transgenic oilseed rape around Japanese ports and roadsides

Published online by Cambridge University Press:  22 June 2006

Hikaru Saji ,
Nobuyoshi Nakajima ,
Mitsuko Aono ,
Masanori Tamaoki ,
Akihiro Kubo ,
Seiji Wakiyama ,
Yoriko Hatase  and
Masato Nagatsu
Hikaru Saji
Affiliation:
 Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Nobuyoshi Nakajima
Affiliation:
 Biodiversity Conservation Research Project, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Mitsuko Aono
Affiliation:
 Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Masanori Tamaoki
Affiliation:
 Biodiversity Conservation Research Project, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Akihiro Kubo
Affiliation:
 Environmental Biology Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, 305-8506, Japan
Seiji Wakiyama
Affiliation:
 Japan Wildlife Research Center, 3-10-10 Shitaya, Taito-Ku, Tokyo, 110-8676, Japan
Yoriko Hatase
Affiliation:
 Japan Wildlife Research Center, 3-10-10 Shitaya, Taito-Ku, Tokyo, 110-8676, Japan
Masato Nagatsu
Affiliation:
 Japan Wildlife Research Center, 3-10-10 Shitaya, Taito-Ku, Tokyo, 110-8676, Japan

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

An investigation was carried out to monitor the escape and spread of oilseed rape (Brassica napus) transgenic plants and the introgression of transgenes to its closely related feral species in Japan. We screened a total of about 7500 feral B. napus, 300 B. rapa, and 5800 B. juncea seedlings from maternal plants in 143 locations at several ports, roadsides, and riverbanks. The presence of glufosinate-resistance or glyphosate-resistance transgenes in these seedlings was confirmed by means of herbicide treatments and also immunochemical and DNA analyses. B. napus plants with herbicide-resistant transgenic seeds were found at five of six major ports and along two of four sampled roadsides in the Kanto District. Transgenic oilseed rape plants have not been commercially cultivated in Japan, suggesting that the transgenes would probably have come from imported transgenic seeds that were spilled during transportation to oilseed processing facilities. No transgenes were detected in seeds collected from B. napus plants growing along riverbanks in the Kanto District or in seeds from closely related species (B. rapa and B. juncea). To our knowledge, this is the first published example of feral, transgenic populations occurring in a nation where the transgenic crop has not been cultivated commercially.

Keywords

Brassicaestablishmentglufosinateglyphosateherbicideintrogressiontransgenic plant
Type
Research Article
Information
Environmental Biosafety Research , Volume 4 , Issue 4 , October 2005 , pp. 217 - 222
Copyright
© ISBR, EDP Sciences, 2006

References

Bing, DJ, Downey, RK, Rakow, GFW (1996) Hybridizations among Brassica napus, B. rapa and B. juncea and their two weedy relatives B. nigra and Sinapis arvensis under open pollination conditions in the field. Plant Breed. 115: 470–473 CrossRef
Brown, J, Brown, AP (1996) Gene transfer between canola (Brassica napus L. and B. campestris L.) and related weed species. Annal. Appl. Biol. 129: 513–522 CrossRef
Comai, L, Facciotti, D, Hiatt, WR, Thompson, G, Rose, RE, Stalker, DM (1985) Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317: 741–744 CrossRef
Crawley, MJ, Brown, SL (1995) Seed limitation and the dynamics of feral oilseed rape on the M25 motorway. Proc. Royal Soc. London B 259: 49–54 CrossRef
Crawley, MJ, Brown, SL, Hails, RS, Kohn, DD, Rees, M (2001) Transgenic crops in natural habitats. Nature 409: 682–683 CrossRef
EFSA (2004) Guidance document of the scientific panel on genetically modified organisms for the risk assessment of genetically modified plants and derived food and feed. http://www.efsa.eu.int/science/gmo/gmo_guidance/660_en.html
Frello, S, Hansen, KR, Jensen, J, Jørgensen, RB (1995) Inheritance of rapeseed (Brassica napus)-specific RAPD markers and a transgene in the cross B. juncea × (B. juncea × B. napus). Theor. Appl. Genet. 91: 236–241 CrossRef
Hansen LB, Siegismund HR, Jørgensen RB (2003) Progressive introgression between Brassica napus (oilseed rape) and B. rapa. Heredity 91: 276–283 CrossRef
ISAAA (2005) Preview: Global Status of Commercialized Biotech/GM Crops: 2004. http://www.isaaa.org/
Jørgensen, RB, Andersen, B, Landbo, L, Mikkelsen, TR (1996) Spontaneous hybridization between oilseed rape (Brassica napus) and weedy relatives. Acta Horticul. 407: 193–197 CrossRef
Jørgensen, RB, Andersen, B, Snow, A, Hauser, TP (1999) Ecological risks of growing genetically modified crops. Plant Biotechnol. 16: 69–71 CrossRef
Messeguer, J (2003) Gene flow assessment in transgenic plants. Plant Cell Tissue Organ Culture 73: 201–212 CrossRef
Mikkelsen, TR, Andersen, B, Jørgensen, RB (1996a) The risk of crop transgene spread. Nature 380: 31 CrossRef
Mikkelsen TR, Jensen J, Jørgensen RB (1996b) Inheritance of oilseed rape (Brassica napus) RAPD markers in a backcross progeny with Brassica campestris. Theor. Appl. Genet. 92: 492–497
Nakajima, N, Kakagawa, N, Imaseki, H (1988) Molecular size of wound-induced 1-aminocyclopropane-1-carboxylate synthase from Cucurbita maxima Duch. and change of translatable mRNA of the enzyme after wounding. Plant Cell Physiol. 29: 989–998
OGTR (2002) The biology and ecology of canola (Brassica napus). http://www.ogtr.gov.au/pdf/ir/brassica.pdf
Pessel, FD, Lecomte, J, Emeriau, V, Krouti, M, Messéan, A, Gouyon, PH (2001) Persistence of oilseed rape (Brassica napus L.) outside of cultivated fields. Theor. Appl. Genet. 102: 841–846 CrossRef
Snow AA, Andersen B, Jørgensen RB (1999) Costs of transgenic herbicide resistance introgressed from Brassica napus into weedy B. rapa. Mol. Ecol. 8: 605–615 CrossRef
Thompson CJ, Movva NR, Tizard R, Crameri R, Davies JE, Lauwereys M, Botterman J (1987) Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO J. 6: 2519–2523
Warwick, SI, Simard, MJ, Légère, A, Beckie, HJ, Braun, L, Zhu, B, Mason, P, Séguin-Swartz, G, Stewart, CN (2003) Hybridization between transgenic Brassica napus L. and its wild relatives: Brassica rapa L., Raphanus raphanistrum L., Sinapis arvensis L., and Erucastrum gallicum (Willd.) O.E. Schulz. Theor. Appl. Genet. 107: 528–539 CrossRef
Warwick SI, Beckie HJ, Simard MJ, Légère A, Nair H, Séguin-Swartz G (2004) Environmental and agronomic consequences of herbicide-resistant (HR) canola in Canada. In Den Nijs HCM, Bartsch D, Sweet J, eds, Introgression from Genetically Modified Plants into Wild Relatives, CABI, pp 323–337