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Chemical additives that affect the potency of endotoxin of Bacillus thuringiensis against Plodia interpunctella

Published online by Cambridge University Press:  19 September 2011

A. El-Moursy ,
R. Aboul-Ela ,
H. S. Salama  and
A. Abdel-Razek
A. El-Moursy
Affiliation:
Faculty of Science, Cairo University, Cairo, Egypt
R. Aboul-Ela
Affiliation:
Faculty of Science, Cairo University, Cairo, Egypt
H. S. Salama
Affiliation:
National Research Centre, Tahrir St., Dokki, Cairo, Egypt
A. Abdel-Razek
Affiliation:
National Research Centre, Tahrir St., Dokki, Cairo, Egypt
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Abstract

Biochemical approaches have been used to enhance the potency of Bacillus thuringiensis against Plodia interpunctelia. Tests were made with different classes of chemical compounds, characterizedby being safe, non-toxic to man, and cheap in price. Among the tested inorganic salts, calcium carbonate, calcium oxide and zinc sulphate were found to increase the potency of B. thuringiensis δ-endotoxin by 2.5-, 1.9- and 2.7-fold, respectively. With the nitrogenous compounds, the amino acids serlne, arginine, alanine and teucine enhanced the endotoxin effect by 52-, 3.6-, 13- and 1.1-fold, respectively. Also, among the protein solubilizing agents, EDTA and sodium thloglycollate led to a 100- and 2.6-fold Increase in the potency. Formic acid was highly effective among the organic acids used in enhancing the potency, followed by malk acid, with 4.8- and 2.4-fold increase, respectively.

Résumé

Une approche bio-medical a été utilisé pour amellorer la puissance de Bacillus thuringiensis contre la mitte Indienne Plodia interpunctella. Des analyses ont été faites avec des classes de composition chimique characterisé par leur aspect sain. Entre les sets analyses le carbonate de calcium et l'oxide de calcium et la sulphate de zinc ont augmenté la puissance de B. thuringiensis δ-endotoxin par 2, 5, 2, 7 et 1, 9, respectivement. Avec les composition (azotique) les acide amino, la serine, l'arginine, l'alanine et le leucine ont améliore l'effet par par 5, 2, 3, 6, 1, 1 et 1, 3 respectivement. Aussi avec les agents de solubilisation de proteine, EDTA et le thioglycollate de sodium ont augmenté la puissance par 100- et 2, 6 fols. L'acide fourmique a été très efficace parmi les acides organiques qui ont été utilise pour ameliorer la puissance suivi par l'acid malic par 4, 8 et 2, 4 fois, respectivement.

Keywords

AdditivespotencyBacillus thuringiensisPlodia interpunctella
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 13 , Issue 6 , December 1992 , pp. 775 - 779
Copyright
Copyright © ICIPE 1992

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