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Effects of pre-storage hydration treatments on germination performance, moisture content, DNA synthesis and controlled deterioration tolerance of tomato (Lycopersicon esculentum Mill.) seeds

Published online by Cambridge University Press:  19 September 2008

Jaap G. van Pijlen ,
Steven P. C. Groot ,
H. Lieke Kraak ,
Jan H. W. Bergervoet  and
Raoul J. Bino
Jaap G. van Pijlen
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, Netherlands
Steven P. C. Groot*
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, Netherlands
H. Lieke Kraak
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, Netherlands
Jan H. W. Bergervoet
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, Netherlands
Raoul J. Bino
Affiliation:
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA Wageningen, Netherlands
*
*Correspondnce
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Abstract

Storage of tomato seeds under deteriorating conditions reduced the rate of germination, the uniformity of germination, total germination and the proportion of normal seedlings. Pre-storage humidification and hydropriming of seeds resulted in a significant increase in resistance to deterioration. In contrast, osmoprimed seeds were more deterioration sensitive. Humidification or hydropriming for one day did not allow nuclei to enter the S phase of the cell cycle. Osmopriming strongly increased the percentage of nuclei with replicated DNA in the embryonic root tip, indicating initiation of the cell cycle and progression towards the G2 phase. The interaction between the prestorage treatments, cell cycle progression and deterioration resistance, is discussed. Based on the changes in moisture content equilibrium and cell cycle activity it is hypothesized that the beneficial effects of pre-storage humidification and hydropriming were related to metabolic activities induced by the partial hydration and that the adverse effects of osmopriming were caused by a decrease in DNA repair activity due to progression in the cell cycle.

Keywords

DNA replicationgerminationhydration treatmentshysteresismoisture contentrelative humidityseed deteriorationseed storagetomato seed
Type
Physiology and Biochemistry
Information
Seed Science Research , Volume 6 , Issue 2 , June 1996 , pp. 57 - 63
Copyright
Copyright © Cambridge University Press 1996

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