Hostname: page-component-8448b6f56d-jr42d Total loading time: 0 Render date: 2024-04-19T08:51:44.046Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphological diversity and genomic DNA fingerprinting of the African rice gall midge Orseolia oryzivora (Diptera: Cecidomyiidae) and of two other species of African Orseolia

Published online by Cambridge University Press:  01 December 2006

Francis E. Nwilene ,
Keith M. Harris ,
Olusegun Okhidievbie ,
Amos Onasanya ,
Yacouba Sere  and
Ivan Ingelbrecht
Francis E. Nwilene*
Affiliation:
Africa Rice Center, PO Box 5320, Ibadan, Nigeria
Keith M. Harris
Affiliation:
Africa Rice Center, 81 Linden Way, Ripley, Woking, Surrey, GU23 6LP, UK
Olusegun Okhidievbie
Affiliation:
Africa Rice Center, PO Box 5320, Ibadan, Nigeria
Amos Onasanya
Affiliation:
Africa Rice Center, 01 BP 2031, Cotonou, Benin Republic
Yacouba Sere
Affiliation:
Africa Rice Center, 01 BP 2031, Cotonou, Benin Republic
Ivan Ingelbrecht
Affiliation:
International Institute of Tropical Agriculture, PO Box 5320, Ibadan, Nigeria
*
* E-mail: f.nwilene@cgiar.org
Get access

Abstract

A morphological diversity study was conducted on African rice gall midge Orseolia oryzivora Harris & Gagné and two other African species, Orseolia bonzii Harris and Orseolia nwanzei Harris & Nwilene, sp. n. Morphological differences between adult and immature stages of the three species are slight and mainly evident in pupal characters. Due to limited differences in morphological characters, genomic DNA fingerprinting of these three insect species was carried out using random amplified polymorphic DNA (RAPD) and sequence-characterized amplified region (SCAR) methods. Out of 90 operon primers, 15 showed polymorphism among the three species tested, generating 86 bands, 60 (70%) of which were polymorphic. Primer OPV6 produced three RAPD markers that distinguished the three Orseolia species. These markers were cloned and sequenced. Their sequence was then used to design six SCAR primer pairs. Each SCAR primer pair amplified and distinguished the three Orseolia species at genomic DNA level. Both SCAR and RAPD genomic DNA fingerprinting revealed that O. oryzivora and O. bonzii are most closely related and O. nwanzei is distinct. Each of the six SCAR primer pairs produced a specific DNA fragment size specific for O. nwanzei, O. oryzivora and O. bonzii. The DNA fingerprints will be useful for entomological survey, identification of new species and differentiating aggressive from non-aggressive species, aimed at the effective development of rice cultivars with durable resistance to African rice gall midge.

Une étude de la diversité morphologique a été réalisée sur la cécidomyie de la galle du riz africain, Orseolia oryzivora Harris & Gagné et sur deux autres espèces africaines, O. bonzii Harris et O. nwanzei Harris & Nwilene, sp. n. Les différences morphologiques entre les stades adultes et immatures des trois espèces sont minimes et plus marquées au niveau des caractères nymphaux. A cause des caractères morphologiques limités, la cartographie peptidique de l'ADN génomique de ces trois espèces d'insectes a été effectuée à l'aide de la méthode d'amplification aléatoire d'ADN polymorphe (RAPD) et de la méthode SCAR. Quinze des 90 amorces d'opérons ont montré un polymorphisme chez les trois espèces testées, créant 86 bandes, dont 60 (70%) étaient polymorphes. L'amorce OPV6 a produit trois marqueurs RAPD, qui ont permis de différencier les trois espèces d'Orseolia. Ces marqueurs ont été clonés et séquencés. Leur séquence a été ensuite utilisée pour concevoir six paires d'amorces SCAR. Les paires des amorces de chaque marqueur SCAR ont permis d'amplifier et de différencier les trois espèces d'Orseolia au niveau de l'ADN génomique. La cartographie peptidique de l'ADN génomique des marqueurs SCAR et RAPD a révélé que les espèces O. oryzivora et O. bonzii sont plus étroitement liées et que O. nwanzei est distincte. Chacune des six paires d'amorces SCAR a produit un segment d'ADN de taille spécifique pour O. nwanzei, O oryzivora et O, bonzii; ces paires d'amorces pourraient donc être utiles pour des diagnostiques de terrain. La cartographie peptidique de l'ADN définie pour chaque espèce d'Orseolia sera utile pour les études entomologiques, l'identification de nouvelles espèces et la différentiation des espèces agressives des espèces non agressives, en vue de développer de façon efficace des cultivars de riz dotés d'une résistance durable à la cécidomyie de la galle du riz africain.

Keywords

cécidomyie de la galle du riz africainOrseolia oryzivoraOrseolia bonziiOrseolia nwanzeiADNcartographie peptidiqueSCARrizEragrostis atrovirensAfrican rice gall midgeOrseolia oryzivoraOrseolia bonziiOrseolia nwanzeiDNAfingerprintingSCARriceEragrostis atrovirens
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 26 , Issue 4 , December 2006 , pp. 256 - 265
Copyright
Copyright © ICIPE 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alam, M. S., Kaung Zan, and Alluri, K. (1985) Gall midge (GM) Orseolia oryzivora H & G in Zambia. International Rice Research Newsletter 10 (2), 15–16.Google Scholar
Armstrong, K. A. and Wratten, S. D. (1996) The use of DNA analysis and the polymerase chain reaction in the study of introduced pests in New Zealand, pp. 231–263. In The Ecology of Agricultural Pests: Biochemical Approaches (Edited by W. O. C., Symondson and J. E., Liddell). Chapman and Hall, London.Google Scholar
Bonzi, S. M. (1980) Wild host plants of the rice gall midge Orseolia oryzae W.M. (Diptera: Cecidomyiidae) in Upper Volta. West Africa Rice Development Association Technical Newsletter 2, 5–6.Google Scholar
Dakouo, D., Nacro, S. and Sie, M. (1988) Evolution saisonnière des infestations de la cécidomyie du riz, Orseolia oryzivora H. et G. (Diptera, Cecidomyiidae) dans le sud-ouest du Burkina Faso. Insect Science and Its Application 9, 469–473.Google Scholar
Descamps, M. (1956) Deux diptères nuisibles au riz dans le Nord Cameroun, Pachydiplosis oryzae Wood-Mason et Pachylophus sp. aff lugens Loew. Phytiatrie-Phytopharmacie 5, 109–116.Google Scholar
Gagné, R. J. (1985) A taxonomic revision of the Asian rice gall midge, Orseolia oryzae (Wood-Mason), and its relatives (Diptera: Cecidomyiidae). Entomography 3, 127–162.Google Scholar
Gagné, R. J. (2004) A catalog of the Cecidomyiidae (Diptera) of the world. Memoirs of the Entomological Society of Washington 25, 1–408.Google Scholar
Guthrie, P. A. I., Magill, C. W., Frederiksen, R. A. and Odvody, G. N. (1992) Random amplified polymorphic DNA markers: A system for identifying and differentiating isolates of Colletotrichum graminicola. Phytopathology 82, 832–835.CrossRefGoogle Scholar
Hardrys, H., Balick, M. and Schierwater, B. (1992) Application of random amplified polymorphic DNA (RAPD) in molecular ecology. Molecular Ecology 1, 55–63.CrossRefGoogle Scholar
Harris, K. M. and Gagné, R. J. (1982) Description of the African rice gall midge Orseolia oryzivora sp. n., with comparative notes on the Asian rice gall midge, O. oryzae (Wood-Mason) (Diptera: Cecidomyiidae). Bulletin of Entomological Research 72, 467–472.CrossRefGoogle Scholar
Harris, K. M., Williams, C. T., Okhidievbie, O., LaSalle, J. and Polaszek, A. (1999) Description of a new species of Orseolia (Diptera: Cecidomyiidae) from Paspalum in West Africa, with notes on its parasitoids, ecology and relevance to natural biological control of the African rice gall midge, O. oryzivora. Bulletin of Entomological Research 89, 441–448.CrossRefGoogle Scholar
Helentjaris, T., Slocum, M. and Schaefer, A. (1986) Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms. Theoretical and Applied Genetics 72, 761–769.CrossRefGoogle ScholarPubMed
Jaccard, P. (1908) Nouvelles recherches sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles 44, 223–270.Google Scholar
Jung, G., Skroch, P. W., Nienhuis, J., Coyne, D. P., Arnaud-Sartana, E., Ariyarathne, H. M. and Marita, J.M. (1999) Confirmation of QTL associated with bacterial blight resistance in four different genetic backgrounds in common bean. Crop Science 39, 1448–1455.Google Scholar
Kazan, K., Manner, J. M. and Cameron, D. F. (1993) Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes. Genome 36, 51–56.CrossRefGoogle Scholar
Nicol, D., Armstrong, K. F., Wratten, S. D., Cameron, C. M., Frampton, C. M. and Fenton, B. (1997) Genetic variation in an introduced aphid pest (Metopolophium dirhodum) in New Zealand and relation to individuals from Europe. Molecular Ecology 6, 255–265.CrossRefGoogle Scholar
Nwilene, F. E., Williams, C. T., Ukwungwu, M. N., Dakouo, D., Nacro, S., Hamadoun, A., Kamara, S. I., Okhidievbie, O., Abamu, F. J. and Adam, A. (2002) Reactions of differential rice genotypes to African rice gall midge in West Africa. International Journal of Pest Management 48, 195–201.CrossRefGoogle Scholar
Paran, I. and Michelmore, R. W. (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theoretical and Applied Genetics 85, 985–993.CrossRefGoogle ScholarPubMed
Rohlf, F. J. (1993) NTSYS-pc, Numerical Taxonomy and Multivariate Analysis System. NTSYSExeter, New York.Google Scholar
Shufran, K. A., Black, I. V. W. C. and Margolies, D. C. (1991) DNA fingerprinting to study spatial and temporal distributions of an aphid, Schizaphis graminum (Homoptera: Aphididae). Bulletin of Entomological Research 81, 303–313.CrossRefGoogle Scholar
Sneath, P. H. A. and Sokal, R. R. (1973) Numerical Taxonomy, The Principles and Practice of Numerical Classification. Freeman, San Fransisco, 573 pp..Google Scholar
Swofford, D. L. and Olsen, G. J. (1990) Phylogenetic reconstruction, pp. 411–501. In Molecular Systematics (Edited by D. M., Hillis and C., Moritz). Sinauer Associates, Sunderland.Google Scholar
Taylor, D. B. and Szalanski, A. L. (1999) Identification of Muscidifurax spp. by polymerase chain reaction–restriction fragment length polymorphism. Biological Control 15, 270–273.CrossRefGoogle Scholar
Taylor, D. R., Fomba, S. N., Fannah, S. J. and Bernard, H. M. (1995) African rice gall midge in Sierra Leone. International Rice Research Newsletter 20 (1), 27.Google Scholar
Thottappilly, G., Mignouna, H. D., Onasanya, A., Abang, M., Oyelakin, O. and Singh, N. K. (1999) Identification and differentiation of isolates of Colletotrichum gloeosporioides from yam by random amplified polymorphic DNA markers. African Crop Science Journal 7, 195–205.Google Scholar
Ukwungwu, M. N. and Joshi, R. C. (1992) Distribution of the African rice gall midge, Orseolia oryzivora Harris and Gagné and its parasitoids in Nigeria. Tropical Pest Management 38, 241–244.CrossRefGoogle Scholar
Ukwungwu, M. N., Winslow, M. D. and John, V. T. (1989) Severe outbreak of rice gall midge (GM) in the savanna zone, Nigeria. International Rice Research Newsletter 14 (4), 36–37.Google Scholar
Welsh, J. and McClelland, M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 24, 7213–7218.Google Scholar