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Development of intramuscular fat in Wagyu beef cattle depends on adipogenic or antiadipogenic substances present in serum

Published online by Cambridge University Press:  02 September 2010

S. Torii ,
T. Matsui  and
H. Yano
S. Torii
Affiliation:
Department of Animal Science, Faculty of Agriculture, Kyoto University, Kyoto 606-01, Japan
T. Matsui
Affiliation:
Department of Animal Science, Faculty of Agriculture, Kyoto University, Kyoto 606-01, Japan
H. Yano
Affiliation:
Department of Animal Science, Faculty of Agriculture, Kyoto University, Kyoto 606-01, Japan
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Abstract

In blood, there are many kinds of adipogenic or antiadipogenic factors such as hormones and vitamins. In this study, adipogenic activity in sera of fattened beef cattle was evaluated using cultured mouse 3T3-L1 preadipocytes. After the preadipocytes were grown to reach confluence, serum of fattened beef cattle was added into the culture medium (10%, vol/vol)for 3 days, and thereafter cellular sn-glycerol-3-phosphate dehydrogenase (GPDH) activity was determined as an index ofadipocyte differentiation. Sera were collected from 19 beef cattle (Wagyu and Wagyu × Holstein cross cattle) from three different farms at slaughter. Cellular GPDH activity was significantly different among the farms, and was affected by sex difference (i.e. sera from fattened heifers induced higher GPDH activity than those from steers). There was a positive correlation between GPDH activity and beef marbling performance (T = 0·62, P < 0·02), suggesting that serum factor(s) play a role in development of intramuscular fat deposition. Adipogenic activity was negatively correlated with serum retinol concentration (r = −0·73, P < 0·001). Neither serum cholesterol, triacylglycerol nor non-esterified fatty acid was related to adipogenic activity.

Furthermore, serum retinol concentration was negatively correlated with beef marbling performance. These data imply that retinol level in blood during the fattening period may influence intramuscular fat deposition of beef cattle through its antiadipogenic action on preadipocytes present in muscle tissues.

Keywords

adipocytesdifferentiationintramuscular fatpreadipocytesretinol
Type
Research Article
Information
Animal Science , Volume 63 , Issue 1 , August 1996 , pp. 73 - 78
Copyright
Copyright © British Society of Animal Science 1996

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References

Abe, K., Ishibashi, K., Ohmae, M., Kawabe, Y. and Katsui, G. 1977. Determination of vitamin A in serum and liver by high-speed liquid chromatography. Vitamins (in Japanese with English abstr.) 51: 275–280.Google Scholar
Akanbi, K. A., Brodie, A. E., Suryawan, A. and Hu, C. Y. 1994. Effect of age on the differentiation of porcine adipose stromal-vascular cells in culture. Journal of Animal Science 72: 2828–2835.CrossRefGoogle ScholarPubMed
Akanbi, K. A. and Hu, C. Y. 1995. Effect of sera from lean and obese pigs on the differentiation of porcine adipose stromal-vascular cells in culture. Comparative Biochemistry and Physiology 111A: 293–298.CrossRefGoogle Scholar
Amri, E. Z., Ailhaud, G. and Grimaldi, P. A. 1994. Fatty acids as signal transducing molecules: involvement in the differentiation of preadipose to adipose cells. Journal of Lipid Research 35: 930–937.CrossRefGoogle ScholarPubMed
Amri, E. Z., Bonino, F., Ailhaud, G., Abumrad, N. A. and Grimaldi, P. A. 1995. Cloning of a protein that mediates transcriptional effects of fatty acids in preadipocytes. Journal of Biological Chemistry 270: 2367–2371.CrossRefGoogle ScholarPubMed
Björntorp, P., Faust, I. M., Miller, W. H., Karlsson, M., Sypniewska, G. and Dahlgren, K. 1985. Dietary and species influence on potential of plasma to stimulate differentiation and lipid accumulation in cultured adipocyte precursors. Journal of Lipid Research 26: 1444–1454.CrossRefGoogle ScholarPubMed
Cianzio, D. S., Topel, D. G., Whitehurst, G. B., Beitz, D. C. and Self, H. L. 1985. Adipose tissue growth and cellularity: changes in bovine adipocyte size and number. Journal of Animal Science 60: 970–976.CrossRefGoogle ScholarPubMed
Darimont, C., Gaillard, D., Ailhaud, G. and Negrel, R. 1993. Terminal differentiation of mouse preadipocyte cells: adipogenic and antimitogenic role of triiodothyronine. Molecular and Cellular Endocrinology 98: 67–73.CrossRefGoogle ScholarPubMed
Fletcher, M. J. 1968. A colorimetric method for estimating serum triglycerides. Clinica Chimica Acta 22: 393–397.CrossRefGoogle ScholarPubMed
Gregoire, F., Genart, C., Hauser, N. and Remade, C. 1991. Glucocorticoids induce a drastic inhibition of proliferation and stimulate differentiation of adult rat fat cell precursors. Experimental Cell Research 196: 270–278.CrossRefGoogle ScholarPubMed
Hauner, H., Wabitsch, M., Zwiauer, K., Widhalm, K. and Pfeiffer, E. F. 1989. Adipogenic activity in sera from obese children before and after weight reduction. American Journal of Clinical Nutrition 50: 63–67.CrossRefGoogle ScholarPubMed
Hausman, G. J., Campion, D. R. and Martin, R. J. 1980. Search for the adipocyte precursor cell and factors that promote its differentiation. Journal of Lipid Research 21: 657–670.CrossRefGoogle ScholarPubMed
Hood, R. L. and Allen, C. E. 1973. Cellularity of bovine adipose tissue. Journal of Lipid Research 14: 605–610.CrossRefGoogle ScholarPubMed
Ishida, Y., Taniguchi, H. and Baba, S. 1988. Possible involvement of 1α, 25-dihydroxyvitamin D3 in proliferation and differentiation of 3T3-L1 cells. Biochemical and Biophysical Research Communications 151: 1122–1127.CrossRefGoogle Scholar
Japan Meat Grading Association. 1988. New beef carcass grading standards. Japan Meat Grading Association, Tokyo, Japan.Google Scholar
Jewell, D. E. and Hausman, G. J. 1988. Sera-controlled preadipocyte growth in culture: an ontogeny study in sera from lean and obese pigs. Journal of Animal Science 66: 2393–2400.CrossRefGoogle ScholarPubMed
Johnson, P. R. and Francendese, A. A. 1985. Cellular regulation of adipose tissue growth. Journal of Animal Science 61: (suppl. 2) 57–75.CrossRefGoogle Scholar
Kawada, T., Aoki, N., Kamei, Y., Maeshige, K., Nishiu, S. and Sugimoto, E. 1990. Comparative investigation of vitamins and their analogues on terminal differentiation, from preadipocytes to adipocytes, of 3T3-L1 cells. Comparative Biochemistry and Physiology 96A: 323–326.CrossRefGoogle Scholar
Kuri-Harcuch, W. and Green, H. 1978. Adipose conversion of 3T3 cells depends on a serum factor. Proceedings of the National Academy of Sciences of the United States of America 75: 6107–6109.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193: 265–275.CrossRefGoogle ScholarPubMed
Maehata, E. and Naka, H. 1972. [The colorimetric determination of non esterified fatty acid (NEFA) with 2-(2-thiazo)-p-cresol.] Japanese Journal of Clinical Chemistry 1: 447–456.Google Scholar
Nakai, A., Kita, K., Hasegawa, K. and Hiramitsu, M. 1992. [Vitamin A deficiency in Japanese Black Cattle.] Journal of Clinical Veterinary Medicine 10: 2143–2148.Google Scholar
Oka, A., Miki, T., Maruo, Y., Yamazaki, M., Ariyoshi, T. and Fujii, H. 1992. [Effects of vitamin A administration on meat quality of Japanese Black steers.] Journal of Clinical Veterinary Medicine 10: 2152–2158.Google Scholar
Pairault, J., Quignard-Boulange, A., Dugail, I. and Lasnier, F. 1988. Differential effects of retinoic acid upon early and late events in adipose conversion of 3T3 preadipocytes. Experimental Cell Research 177: 27–36.CrossRefGoogle ScholarPubMed
Ramsay, T. G., Hausman, G. J. and Martin, R. J. 1987. Pre-adipocyte proliferation and differentiation in response to hormone supplementation of decapitated fetal pig sera. Journal of Animal Science 64: 735–744.CrossRefGoogle ScholarPubMed
Safonova, I., Darimont, C., Amri, E. Z., Grimaldi, P., Ailhaud, G., Reichert, U. and Shroot, B. 1994. Retinoids are positive effectors of adipose cell differentiation. Molecular and Cellular Endocrinology 104: 201–211.CrossRefGoogle ScholarPubMed
Sato, M. and Hiragun, A. 1988. Demonstration of 1α, 25-dihydroxyvitamin D3 receptor-like molecule in ST 13 and 3T3 LI preadipocytes and its inhibitory effects on preadipocyte differentiation. Journal of Cellular Physiology 135: 545–550.CrossRefGoogle Scholar
Sato, M., Hiragun, A. and Mitsui, H. 1980. Preadipocytes possess cellular retinoid binding proteins and their differentiation is inhibited by retinoids. Biochemical and Biophysical Research Communications 95: 1839–1845.CrossRefGoogle ScholarPubMed
Statistical Analysis Systems Institute, 1989. SAS user's guide, release 6.07. SAS Institute Inc., Cary, NC.Google Scholar
Tontonoz, P., Hu, E., Graves, R. A., Budavari, A. I. and Spiegelman, B. M. 1994. mPPARγ2: tissue-specific regulator of an adipocyte enhancer. Genes and Development 8: 1224–1234.CrossRefGoogle ScholarPubMed
Torii, S., Matsuda, M., Matsui, T. and Yano, H. 1995. Primary culture of preadipocytes from beef cattle and its differentiation into adipocytes. Bulletin of Beef Cattle Science (in Japanese with English abstr.) 59: 25–30.Google Scholar
United States Department of Agriculture. 1989. Official United States standards for grades of carcass beef. Agricultural Marketing Service, USDA, Washington, DC.Google Scholar
Wiederer, O. and Loffler, G. 1987. Hormonal regulation of the differentiation of rat adipocyte precursor cells in primary culture. Journal of Lipid Research 28: 649–658.CrossRefGoogle ScholarPubMed
Wise, L. S. and Green, H. 1979. Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells. Journal of Biological Chemistry 254: 273–275.CrossRefGoogle ScholarPubMed
Zurkowski, P. 1964. A rapid method for cholesterol determination with a single reagent. Biological Chemistry 10: 451–453Google ScholarPubMed