Hostname: page-component-8448b6f56d-dnltx Total loading time: 0 Render date: 2024-04-24T01:26:42.690Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of vitamin A deficiency on the immune response to epizootic diarrhoea of infant mice (EDIM) rotavirus infection in mice

Published online by Cambridge University Press:  09 March 2007

Faruk Ahmed ,
David B. Jones  and
Alan A. Jackson
Faruk Ahmed
Affiliation:
Department of Human NutritionUniversity of Southampton, Bassett Crescent East, Southampton S09 3TU
David B. Jones
Affiliation:
Department of Pathology, University of Southampton, Bassett Crescent East, Southampton S09 3TU
Alan A. Jackson
Affiliation:
Department of Human NutritionUniversity of Southampton, Bassett Crescent East, Southampton S09 3TU
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effect of vitamin A deficiency on the immune response to epizootic diarrhoea of infant mice (EDIM) rotavirus was studied in mice. The virus was given by oral dosing or by intraperitoneal injection. For oral challenge, weanling mice were fed on either a control or vitamin A-deficient diet ad lib. or pair-fed the control diet to the intake of the vitamin A-deficient group. A fourth group was fed on the vitamin Adeficient diet ad lib. for 10 weeks and then refed the control diet for 2 weeks. On day 77, mice were each given 30 μl EDIM rotavirus orally and the animals were killed and examined 1 week later. The delayedtype hypersensitivity (DTH) response to picryl chloride was measured as an index of cell-mediated immunity. For intraperitoneal challenge, weanling mice were fed on either the control diet or the vitamin A-deficient diet ad lib. or pair-fed the control diet to the intake of the vitamin A-deficient group. On day 77, mice were each injected intraperitoneally with 30 μl EDIM rotavirus and 1 week later antibody production was measured. In both experiments the body-weight, liver and serum vitamin A levels of the vitamin A-deficient group were significantly lower than the control or pair-fed groups. Following oral dosing the serum antibody levels specific to rotavirus were statistically significantly lower in vitamin Adeficient animals than the control or pair-fed groups. Vitamin A-deficient mice also showed an impaired DTH response compared with the control and pair-fed animals. Animals refed vitamin A for a short period showed a partial restoration of the antibody response. Following intraperitoneal challenge no statistically significant changes were observed in the serum antibody levels between any of the dietary groups. It is concluded that vitamin A deficiency impaired antibody production when rotavirus was given orally. Vitamin A deficiency also impaired cell-mediated immunity.

Keywords

AntibodyImmune responseMouse
Type
Effects of Altered Vitamin Status
Information
British Journal of Nutrition , Volume 65 , Issue 3 , May 1991 , pp. 475 - 485
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Ahmed, F., Jones, D. B. & Jackson, A. A. (1990). The interaction of vitamin A deficiency and rotavirus infection in the mouse. British Journal of Nutrition 63, 363–373.CrossRefGoogle ScholarPubMed
American Institute of Nutrition (1977). Reports of the American Institute of Nutrition Ad Hoc Committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Bang, F. B. & Foard, M. A. (1971). The effect of acute vitamin A deficiency on the susceptibility of chicks to Newcastle disease and influenza viruses. Johns Hopkins Medical Journal 129, 100–109.Google ScholarPubMed
Bayfield, R. F. (1975). Simplified methods for the examination of liver lipids. I. Determination of vitamin A in liver using a direct solvent extraction technique. Analytical Biochemistry 64, 403–413.CrossRefGoogle ScholarPubMed
Bieri, J. G., Tolliver, T. J. & Catignani, G. L. (1979). Simultaneous determination of α-tocopherol and retinol in plasma or red cells by high-pressure liquid chromatography. American Journal of Clinical Nutrition 32, 2143–2149CrossRefGoogle ScholarPubMed
Brown, W. R., Kiyoko, I., Nakane, P. K. & Pacini, B. (1977). Studies on translocation of immunoglobulins across intestinal epithelium. IV. Evidence for binding of IgA and IgM to secretory component in intestinal epithelium. Gastroenterology 73, 1333–1339.CrossRefGoogle ScholarPubMed
Chandra, R. K. & Au, B. (1981). Single nutrient deficiency and cell mediated immune responses. 3. Vitamin A. Nutrition Research 1, 181–185.CrossRefGoogle Scholar
Davis, C. Y. & Sell, J. L. (1983). Effect of all-trans retinol and retinoic acid nutriture on the immune system of chicks. Journal of Nutrition 113, 1914–1919.CrossRefGoogle ScholarPubMed
Goldstone, L. A. (1983). Understanding Medical Statistics. London: William Heinemann Medical Books Ltd.Google Scholar
Harmon, B. G., Miller, E. R., Hoefer, J. A., Ullrey, D. E. & Luecke, R. W. (1963). Relationship of specific nutrient deficiencies to antibody production in swine. 1. Vitamin A. Journal of Nutrition 79, 263–268.CrossRefGoogle Scholar
Kagnoff, M. F., Serfilippi, D. & Donaldson, M. R. (1973). In vitro kinetics of intestinal secretory IgA secretion. Journal of Immunology 110, 297–300.CrossRefGoogle ScholarPubMed
Krishnan, S., Bhuyan, U. N., Talwar, G. P. & Ramalingaswami, V. (1974). Effect of vitamin A and protein-calorie undernutrition on immune responses. Immunology 27, 383–392.Google ScholarPubMed
Krishnan, S., Krishnan, A. D., Mustafa, A. S., Talwar, G. P. & Ramalingaswami, V. (1976). Effect of vitamin A and undernutrition on the susceptibility of rodents to a malarial parasite Plasmodium berghei. Journal of Nutrition 106, 784–791.CrossRefGoogle ScholarPubMed
Ludovici, P. P. & Axelrod, A. E. (1951). Circulating antibodies in vitamin-deficiency states. Pteroylglutamic acid, niacin-tryptophan, vitamin B12, A and D deficiencies. Proceedings of the Society for Experimental Biology and Medicine 77, 526–530.CrossRefGoogle ScholarPubMed
Nauss, K. M., Anderson, C. A., Conner, M. W. & Newberne, P. M. (1985). Ocular infection with Herpes Simplex Virus (HSV-1) in vitamin A-deficient and control rats. Journal of Nutrition 115, 1300–1315.CrossRefGoogle ScholarPubMed
Panda, B. & Combs, G. F. (1963). Impaired antibody production in chicks fed diets low in vitamin A, pantothenic acid or riboflavin. Proceedings of the Society for Experimental Biology and Medicine 113, 530–534.CrossRefGoogle Scholar
Pine, A. (1983). Vitamin A deficiency and child blindness in the developing world. Proceedings of the Nutrition Society 42, 53–64.Google Scholar
Pruzansky, J. & Axelrod, A. E. (1955). Antibody production to diphtheria toxoid in vitamin A deficiency states. Proceedings of the Society for Experimental Biology and Medicine 89, 323–325.CrossRefGoogle Scholar
Roels, O. A. (1970). Vitamin A physiology. Journal of the American Medical Association 214, 1097–1102.CrossRefGoogle ScholarPubMed
Roitt, I. M., Brostoff, J. & Male, D. K. (1985). Immunology. London: Gower Medical Publishing Ltd.Google Scholar
Sirisinha, S., Darip, M. D., Moongkarndi, P., Ongsakul, M. & Lamb, A. J. (1980). Impaired local immune response in vitamin A-deficient rats. Clinical and Experimental Immunology 40, 127–135.Google ScholarPubMed
Smith, S. M. & Hayes, C. E. (1987). Contrasting impairments in IgM and IgG responses of vitamin A-deficient mice. Proceedings of the National Academy of Sciences, USA 84, 5878–5882.CrossRefGoogle ScholarPubMed
Starkey, W. G., Collins, J., Wallis, T. S., Clarke, G. J., Spencer, A. J., Haddon, S. J., Osborne, M. P., Candy, D. C. A. & Stephen, J. (1986). Kinetics, tissue specificity and pathological changes in murine rotavirus infection of mice. Journal of General Virology 67, 2625–2634.CrossRefGoogle ScholarPubMed
Vearman, J. P. & Heremans, J. F. (1970). Origin and molecular size of immunoglobulin A in the mesenteric lymph node of the dog. Immunology 18, 27–38.Google Scholar