Hostname: page-component-848d4c4894-p2v8j Total loading time: 0.001 Render date: 2024-05-14T10:27:09.123Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of a Multiple-Lumen Nerve Cuff Utilizing Growth Stimulant Patterns for Controlled Regeneration

Published online by Cambridge University Press:  15 February 2011

Y.S. Chen ,
C. Miller ,
M.H. Greer ,
M. Quinones  and
R.T. Greer
Y.S. Chen
Affiliation:
Iowa State University, Biomedical Engineering ProgramAmes, IA 50011
C. Miller
Affiliation:
Iowa State University, Biomedical Engineering ProgramAmes, IA 50011
M.H. Greer
Affiliation:
Iowa State University, Biomedical Engineering ProgramAmes, IA 50011 Department of Biomedical SciencesAmes, IA 50011
M. Quinones
Affiliation:
Department of Veterinary Clinical Sciences, and Department of Aerospace Engineering and Engineering Mechanics, Ames, IA 50011
R.T. Greer
Affiliation:
Iowa State University, Biomedical Engineering ProgramAmes, IA 50011
Get access

Abstract

Silicone rubber multiple-lumen (ML) nerve cuffs were used for side-by-side comparisons of the effectiveness of two varieties of nerve growth stimulants (collagen gel and a gel mixture of collagen, fibronectin, and laminin) for regenerating cables across a 15 mm gap in 10 adult Sprague-Dawley rats. The filling pattern of the six tubes of the multiple-lumen portion of the cuff alternated with the collagen gel and the gel mixture of collagen, fibronectin, and laminin. After an 8 week implantation period, 53% (32 of 60) of the 0.5 mm diameter lumens displayed successful cable regeneration for both material fillings. The ML cuff experiments demonstrate the ability to bridge a 15 mm gap in the sciatic nerve of the rat by 8 weeks for relatively small diameter conduits in ML cuffs loaded with two varieties of growth stimulants. A more advanced organization including a smaller acellular region fraction and a higher vascularity is seen in the cables from the mixture-filled lumens compared to those in the cables from the collagen-filled lumens.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 550: Symposium GG/HH/II – Biomedical Materials-Drug Delivery, Implants and Tissue Engr , 1998 , 303
Copyright
Copyright © Materials Research Society 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Williams, L. R., Longo, F. M., Powell, H. C., Lundborg, G., and Varon, S.. J. Comp. Neurol. 218, 460–470 (1983).Google Scholar
2. Rosen, J. M., Pham, H. N., Abraham, G., Harold, L., and Hentz, V. R.. J. Rehab. Res. and Dev. 26(1), 1–14(1989).Google Scholar
3. Weiss, P., J. Neurosurg., 1, 400–450(1944).Google Scholar
4. Lundborg, G., Dahlin, L. B., Danielson, N., Gelberman, R. H., Longo, F. M., Powell, H. C., and Varon, S., Exp. Neuro., 76, 361–375(1982).Google Scholar
5. Lundborg, G., Gelberman, R. H., Longo, F. M., Powell, H. C., and Varon, S., J. Neuropathol., 41, 412–422(1982).Google Scholar
6. Satou, T., Nishida, S., Hiruma, S., Tanji, K., Takahashi, M., Fujita, S., Mizuhara, Y., Akai, F. and Hashimoto, S.. Acta Pathol. Jpn. 36, 199–208(1986).Google Scholar
7. Valentini, R. F., Aebischer, P., Winn, S. R., and Galletti, P. M.. Exp. Neuro. 98, 350–356(1987).Google Scholar
8. Madison, R., Da Silva, C. F., and Dikkes, P., Brain Res. 447, 325–334(1988).Google Scholar
9. Woolley, A. L., Hollowell, J. P., and Rich, K. M.. Otolaryngology-Head and Neck Surgery 103(4), 509–518(1990).Google Scholar
10. Henry, E. W., Chiu, T. H., Nyilas, E., Brushart, T. M., Dikkes, P., and Sidman, R. L., Exp. Neuro., 90, 652–676(1985).Google Scholar
11. Kok, L. P. and Boon, M. E.. Microwave Cookbookfor Microscopists. Art and Science of Visualization, (Coulomb Press Leyden, Leiden, The Netherlands, 1992), pp. 248–250.Google Scholar
12. Kiernan, J. A., Histological and Histochemical Methods. Theory and Practice. (Pergamon Press, New York, 1990), pp. 301–329.Google Scholar
13. Hayat, M. A., Stains and Cytochemical Methods, (Plenum Press, New York, 1993), pp. 318–336.Google Scholar
14. Amillo, S., Yanez, R., and Barrios, R. H., Microsurgery 16, 621–630(1995).Google Scholar
15. Aldini, N. N., Perego, G., Celia, D. D., Maltarello, M. C., Fini, M., Rocca, M., and Giardino, R., Biomaterials 17(10), 959–962(1996).Google Scholar
16. Lundborg, G. and Kanje, M.. Scand. J. Plast. Reconstr. Hand. Surg. 30, 105–110(1996).Google Scholar
17. Longo, F. M., Hayman, E. G., Davis, G. E., Ruoslahti, E., Engall, E., Manthorpe, M., and Varon, S., Brain Res., 309, 105–117(1984).Google Scholar
18. Fields, R. D., Le Beau, I. M., Longo, F. M., and Ellisman, M. H., Progress in Neuorobiology, 33, 87–134(1989).Google Scholar
19. Den Dunnen, W. F. A., Van Der Lei, B., Schakenraad, J. M., Blaauw, E. H., Stokroos, I., Pennings, A. J., and Robinson, P. H., Microsurgery, 14, 508–515(1993).Google Scholar
20. Jenq, C. B. and Coggeshall, R. E., Brain Res., 326, 27–40(1985).Google Scholar
21. Derby, A., Engleman, W., Frierdich, G. E., Neises, G., Rapp, S. R., and Roufa, D. G., Exp. Neuro., 119, 176–191(1993).Google Scholar
22. Bailey, S. B., Eichler, M. E., Villadiego, A., and Rich, K. M., J. Neurocytology, 22, 176–184(1993).Google Scholar