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Microsatellite markers for Cattleya walkeriana Gardner, an endangered tropical orchid species

Published online by Cambridge University Press:  14 January 2016

Evandro Vagner Tambarussi ,
Lou C. Menezes ,
Bruna Ibañes ,
Lia Maris Orth Ritter Antiqueira ,
Gabriel Dequigiovanni ,
Maria Andréia Moreno ,
Elza Martins Ferraz ,
Maria Imaculada Zucchi ,
Elizabeth Ann Veasey  and
Roland Vencovsky
Evandro Vagner Tambarussi*
Affiliation:
Universidade Estadual do Centro-Oeste (UNICENTRO), Departamento de Engenharia Florestal, PR 153, Km 7, 84500-000Irati, PR, Brazil
Lou C. Menezes
Affiliation:
Orquidário Nacional do Ibama (IBAMA), Alameda SCEN Trecho 02, Acesso pela L4 Norte, 70818-900Brasília, DF, Brazil
Bruna Ibañes
Affiliation:
Centro de Energia Nuclear na Agricultura (CENA/USP), Av. Centenário, 303, CP 96, 13400-970Piracicaba, SP, Brazil
Lia Maris Orth Ritter Antiqueira
Affiliation:
Universidade Tecnológica Federal do Paraná (UTFPR), Av. Monteiro Lobato, Km 4, Jardim Carvalho, 84016-210Ponta Grossa, PR, Brazil
Gabriel Dequigiovanni
Affiliation:
Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ/USP), Av. Padua Dias, 11, PO Box 9, 13418-090Piracicaba, SP, Brazil
Maria Andréia Moreno
Affiliation:
Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ/USP), Av. Padua Dias, 11, PO Box 9, 13418-090Piracicaba, SP, Brazil
Elza Martins Ferraz
Affiliation:
Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ/USP), Av. Padua Dias, 11, PO Box 9, 13418-090Piracicaba, SP, Brazil
Maria Imaculada Zucchi
Affiliation:
Agência Paulista de Tecnologia dos Agronegócios, Pólo Regional Centro Sul, Rodovia SP127, Km 30, CP 28, 13400-970Piracicaba, SP, Brazil
Elizabeth Ann Veasey
Affiliation:
Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ/USP), Av. Padua Dias, 11, PO Box 9, 13418-090Piracicaba, SP, Brazil
Roland Vencovsky
Affiliation:
Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ/USP), Av. Padua Dias, 11, PO Box 9, 13418-090Piracicaba, SP, Brazil
*
*Corresponding author. E-mail: tambarussi@gmail.com
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Abstract

Cattleya walkeriana, one of the most improved Brazilian Cattleyas, is a popular tropical orchid endemic from Brazil and currently endangered. In the present study, for the first time microsatellite markers were developed for C. walkeriana and their transferability was tested for the species C. loddigesii and C. nobilior. The markers were used for genotyping 26 C. walkeriana specimens from different growers and from different levels of improvement. The transferability was successful, with five polymorphic loci transferred to C. loddigesii and six polymorphic loci to C. nobilior. Eight loci were polymorphic, revealing a maximum of two to ten alleles per locus in C. walkeriana and two to four and two to five in C. loddigesii and C. nobilior, respectively. There was no significant linkage disequilibrium in the studied loci. For C. walkeriana, the observed and expected heterozygosities ranged from 0 to 0.963 and from 0.138 to 0.841, respectively. These markers identified polymorphisms and may be used to study the genetic diversity, gene flow or hybridization of these species.

Keywords

Brazilian orchidconservation geneticshybridizationpopulation geneticstropical orchid species
Type
Short Communications
Information
Plant Genetic Resources , Volume 15 , Issue 1 , February 2017 , pp. 93 - 96
Copyright
Copyright © NIAB 2016 

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