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Intestinal growth and pathology of Giardia duodenalis assemblage subtype AI, AII, B and E in the gerbil model

Published online by Cambridge University Press:  05 January 2012

E. BÉNÉRÉ ,
T. VAN ASSCHE ,
C. VAN GINNEKEN ,
O. PEULEN ,
P. COS  and
L. MAES
E. BÉNÉRÉ
Affiliation:
Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences; University of Antwerp, B-2020 Antwerp, Belgium
T. VAN ASSCHE
Affiliation:
Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences; University of Antwerp, B-2020 Antwerp, Belgium
C. VAN GINNEKEN
Affiliation:
Laboratory of Veterinary Anatomy, Embryology and Pathology, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences; University of Antwerp, B-2020 Antwerp, Belgium
O. PEULEN
Affiliation:
Metastasis Research Laboratory, GIGA-Cancer, Institute of Pathology, University of Liège, B-4000 Liège, Belgium
P. COS
Affiliation:
Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences; University of Antwerp, B-2020 Antwerp, Belgium
L. MAES*
Affiliation:
Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences; University of Antwerp, B-2020 Antwerp, Belgium
*
*Corresponding author: Laboratory of Microbiology, Parasitology and Hygiene (LMPH), University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Tel: 0032 3 265 26 27. Fax: 0032 3 265 26 81. E-mail: louis.maes@ua.ac.be
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Summary

This study investigated the significance of the genetic differences between assemblages A, B and E on intestinal growth and virulence. Intestinal growth and virulence were studied in 2 laboratory (AI: WB and B: GS/M-83-H7) and 6 field isolates of assemblage subtype AI, AII, B and EIII. Intestinal trophozoite burdens, body weight and faecal consistency were monitored until day 29 post-infection (p.i.), morphological (mucosal architecture and inflammation) and functional (disaccharidase and alkaline phosphatase enzyme activity) damage to the small intestine were evaluated on days 7 and 18 p.i. The assemblage subtypes AI and B were more infectious and produced higher trophozoite loads for a longer period compared to the subtypes AII and EIII. The body weight of infected gerbils was significantly reduced compared to uninfected controls, but did not differ between the assemblage subtypes. Consistent softening of the faeces was only observed with assemblage B. Assemblage B next to assemblage subtype AI elicited relatively higher pathogenicity, characterized by more extensive damage to mucosal architecture, decreased brush-border enzyme function and infiltration of inflammatory cells. Assemblage EIII and AII isolates showed relatively low virulence. The Giardia assemblage subtypes exhibit different levels of growth and virulence in the gerbil model.

Keywords

assemblage subtypes AI, AII, B and Elab and field isolatesgrowth dynamicsvirulencegerbil model
Type
Research Article
Information
Parasitology , Volume 139 , Issue 4 , April 2012 , pp. 424 - 433
Copyright
Copyright © Cambridge University Press 2012

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