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Comparative effects on blood lipids and faecal steroids of five legume species incorporated into a semi-purified, hypercholesterolaemic rat diet

Published online by Cambridge University Press:  09 March 2007

Fatima D Dabai ,
Ann F Walker ,
Ian E Sambrook ,
Vernon A Welch ,
Robert W Owen  and
Savitri Abeyasekera
Fatima D Dabai
Affiliation:
Department of Food Science and Technology, University of Reading, Whiteknights, Reading RG6 2AP
Ann F Walker
Affiliation:
Department of Food Science and Technology, University of Reading, Whiteknights, Reading RG6 2AP
Ian E Sambrook
Affiliation:
Department of Food Science and Technology, University of Reading, Whiteknights, Reading RG6 2AP
Vernon A Welch
Affiliation:
Department of Food Science and Technology, University of Reading, Whiteknights, Reading RG6 2AP
Robert W Owen
Affiliation:
Department of Food Science and Technology, University of Reading, Whiteknights, Reading RG6 2AP
Savitri Abeyasekera
Affiliation:
Department of Applied Statistics, University of Reading, Whiteknights, Reading RG6 2FN
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Abstract

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The cholesterolaemic effects in rats of a diet (VS) containing Bambara groundnuts (Vignu subterrunea), a popular legume eaten in Nigeria, were compared with diets PV, PS, LC and PL, containing baked beans (Phaseolus vulgaris), marrowfat peas (Pisum sativum), lentils (Lens culinaris Medik.) and butter beans (Phaseolus lunatus) respectively. Sixty Sprague-Dawley rats were fed on hypercholesterolaemic semi-purified diets supplemented with 10 g cholesterol and 5 g cholic acid/kg and formulated to provide 40% of energy from fat, as in a typical Western-type human diet. Legumes were substituted for 330 g/kg of the semi-purified diet on a dry-matter basis, which was modified to maintain the same contribution of energy sources as the control diet C3. Another ten rats were fed on control diet C2, which was similar to diet C3 but with no added cholesterol. The rats were fed for 8 weeks and plasma cholesterol levels were measured at weeks 4 and 8. The diets incorporating the five different legume species produced very Merent cholesterolaemic effects. Diets PV and PL were more potent at lowering raised plasma cholesterol levels than diets PS and LC. Inclusion of the Bambara groundnut into the semi-purified diet resulted in an exaggeration of hypercholesterolaemia. Differences in cholesterol-lowering capacity of the various legume diets in this experiment could not be related to concentrations of faecal bile acids or neutral sterols. However, there was evidence that the inclusion of legumes in the diets reduced the faecal excretion of secondary bile acids

Keywords

Hypocholesterolaemic effectSteroidsLipid metabolismLegumes
Type
Legumes and lipid metabolism in rats
Information
British Journal of Nutrition , Volume 75 , Issue 4 , April 1996 , pp. 557 - 571
Copyright
Copyright © The Nutrition Society 1996

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