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Kinetics of large ciliate protozoa in the rumen of cattle given sugar cane diets

Published online by Cambridge University Press:  09 March 2007

R. A. Leng ,
M. Gill ,
T. J. Kempton ,
J. B. Rowe ,
J. V. Nolan ,
S. J. Stachiw  and
T. R. Preston
R. A. Leng
Affiliation:
Department of Biochemistry and Nutrition, University of New England, Armidale, NSW 2351, Australia
M. Gill
Affiliation:
Dominican Centre for Research with Cattle Fed Sugar Cane, CEAGANA, Santo Domingo, Dominican Republic, University of New England, Armidale, NSW 2351, Australia
T. J. Kempton
Affiliation:
Department of Biochemistry and Nutrition, University of New England, Armidale, NSW 2351, Australia
J. B. Rowe
Affiliation:
Dominican Centre for Research with Cattle Fed Sugar Cane, CEAGANA, Santo Domingo, Dominican Republic, University of New England, Armidale, NSW 2351, Australia
J. V. Nolan
Affiliation:
Department of Biochemistry and Nutrition, University of New England, Armidale, NSW 2351, Australia
S. J. Stachiw
Affiliation:
Department of Biochemistry and Nutrition, University of New England, Armidale, NSW 2351, Australia
T. R. Preston
Affiliation:
Dominican Centre for Research with Cattle Fed Sugar Cane, CEAGANA, Santo Domingo, Dominican Republic, University of New England, Armidale, NSW 2351, Australia
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Abstract

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1. Experiments were undertaken to examine the kinetics of large ciliate protozoa in the rumen of cattle on sugar-cane diets.

2. Three Zebu bulls were fed once daily on a diet of sugar cane and wheat bran. The diurnal patterns of volatile fatty acids and ammonia concentrations, and the numbers of protozoa in rumen fluid were determined. The numbers of protozoa reached values of 5 × 104/ml for holotrichs (large ciliates) mainly Isoiricha and Dasytricha spp and 4 × 105 for smaller protozoa, mainiy Entodinia (small ciliates)

3. A method was developed which allowed large ciliate protozoa in rumen fluid to be separated from plant material and bacteria and concentrated in a relatively uncontaminated form. Analysis of these protozoa indicated that 1.8 × 105 large ciliates contained 1 mg nitrogen and approximately 32 mg dry matter

4. A labelled preparation consisting mainly of large ciliates (principally Isotricha spp.) was obtained by incubating isolated protozoa in rumen fluid (free of plant materials) containing [14C-methyl]choline and then isolating them by sedimentation and differential centrifugation

5. A portion of the preparation containing labelled protozoa was incubated in vitro with rumen fluid to determine the turnover of 14C-labelled metabolites. There was no apparent dilution of the label in the protozoa over a 22 h period

6. A major portion of the preparation containing labelled protozoa was returned to the rumen of each of the donor cattle as a single injection. The specific radioactivity in the large protozoa (μCi/mg N) was monitored frequently for over 30 h, and thereafter daily for a further 12 d. The kinetics of tracer dilution were analyzed to give estimates of the size of the pool of these large ciliates in the rumen (24–46 g N), and of their apparent rate of turnover

7. In contrast to the slow turnover of the large ciliates, the rate of turnover of the rumen fluid pool (approximately 54 l), estimated from the rate of dilution of polyethylene glycol, was considerably faster. Large ciliates were therefore selectively retained within the rumen

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 46 , Issue 2 , September 1981 , pp. 371 - 384
Copyright
Copyright © The Nutrition Society 1981

References

Annison, E. F. & White, R. R. (1961). Biochem. J. 80, 162.Google Scholar
Bergen, W. G. & Yokoyama, M. T. (1977). J. Anim. Sci. 46, 573.CrossRefGoogle Scholar
Bird, S. H., Baigent, D. R., Dixon, R. M. & Leng, R. A. (1978). Proc. Soc. Anim. Prod. 12, 137.Google Scholar
Bird, S. H., Hill, M. K. & Leng, R. A. (1979). Br. J. Nutr. 42, 81.CrossRefGoogle Scholar
Bird, S. H. & Leng, R. A. (1978). Br. J. Nutr. 40, 163.CrossRefGoogle Scholar
Broad, T. E. & Dawson, R. M. C. (1976). J. gen. Microb. 92, 391.CrossRefGoogle Scholar
Bruno, G. A. & Christian, J. E. (1961). Analyt. Chem. 33, 650.CrossRefGoogle Scholar
Bygrave, F. L. & Dawson, R. M. C. (1976). Biochem. J. 160, 481.Google Scholar
Clarke, R. T. J. (1965). N.Z. Soc. Anim. Prod. 25, 96.Google Scholar
Coleman, G. S. (1975). In Digestion and Metabolism in the Ruminant, p. 149 [McDonald, I. W. and Warner, A. C. I., editors]. Armidale: University of New England Publishing Unit.Google Scholar
Coleman, G. S. (1979). Trop. Anim. Prod. 4, 199.Google Scholar
Coleman, G. S., Dawson, R. M. C. & Grime, D. W. (1980). Proc. Nutr. Soc. 39, 6A.Google Scholar
Elliott, R., Ferreiro, H. M. & Priego, A. (1978). Trop. Anim. Prod. 3, 36.Google Scholar
Erwin, E. S., Marco, G. J. & Emery, E. M. (1961). J. Dairy Sci. 44, 1768.Google Scholar
Harrison, D. G., Beever, D. E. & Osbourn, D. F. (1979). Br. J. Nutr. 41, 521.Google Scholar
Leng, R. A. (1976). In Reviews in Rural Science, vol. 2, p. 85 [Sutherland, T. M., McWilliam, J. R. and Leng, R. A., editors]. Armidale: University of New England Publishing Unit.Google Scholar
Malawar, S. J. & Powell, D. W. (1967). Gastroenterology 53, 250.Google Scholar
Minor, S., MacLeod, N. A., Preston, T. R. & Leng, R. A. (1977). Trop. Anim. Prod. 2, 163.Google Scholar
Neill, A. R., Grime, D. W. & Dawson, R. M. C. (1978). Biochem. J. 170, 529.Google Scholar
Neill, A. R., Grime, D. W., Snoswell, A. M., Northrop, A. J., Lindsay, D. B. & Dawson, R. M. C. (1979). Biochem. J. 180, 559.Google Scholar
Nolan, J. V. & Leng, R. A. (1972). Br. J. Nutr. 27, 177.Google Scholar
Singh, U. B., Verma, D. N., Varma, A. & Ranjhan, S. K. (1974). J. Dairy Res. 41, 299.Google Scholar
Ulbrich, M. & Scholz, H. (1966). Arch. Tierernahr. 16, 327.Google Scholar
Valdez, R. E., Alvarez, F. J., Ferreiro, H. M., Guerra, F., Lopez, J., Priego, A., Blackburn, T. H., Leng, R. A. & Preston, T. R. (1977). Trop. Anim. Prod. 2, 260.Google Scholar
Van Slyke, D. D. & Folch, J. (1940). J. biol. Chem. 136, 509.Google Scholar
Warner, A. C. I. (1962). J. gen. Microbiol. 28, 129.Google Scholar
Weller, R. A. & Pilgrim, A. F. (1974). Br. J. Nutr. 32, 341.CrossRefGoogle Scholar