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Association of nymphs and adults of Ephemeroptera (Insecta) using the amplified fragment length polymorphism (AFLP) technique

Published online by Cambridge University Press:  22 June 2011

Lucimar G. Dias ,
Tito Bacca ,
Lucio Navarro ,
Flor E. Acevedo ,
Pablo M. Benavides  and
Paulo Sérgio Fiuza Ferreira
Lucimar G. Dias*
Affiliation:
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Colombia
Tito Bacca
Affiliation:
Facultad de Ciencias Agrícolas, Universidad de Nariño, Torobajo, Pasto, Nariño, Colombia
Lucio Navarro
Affiliation:
Disciplina de Entomología, Cenicafé, Chinchiná, Caldas, Colombia
Flor E. Acevedo
Affiliation:
Disciplina de Entomología, Cenicafé, Chinchiná, Caldas, Colombia
Pablo M. Benavides
Affiliation:
Disciplina de Entomología, Cenicafé, Chinchiná, Caldas, Colombia
Paulo Sérgio Fiuza Ferreira
Affiliation:
Museu de Entomologia, Departamento de Biologia Animal, Universidade Federal de Viçosa, Minas Gerais, Brazil
*
*Corresponding author: lucimar.dias@gmail.com
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Abstract

Many species of Ephemeroptera have been described based on either nymphs or adults. Only 10% of Ephemeroptera species in South America are known in both life stages. The current research was carried out in order to molecularly associate nymphs and adults of Ephemeroptera, using the amplified fragment length polymorphism (AFLP) technique. We obtained molecular fingerprints by the AFLP of five species: Leptohyphes albipennis, Leptohyphes coconuco, Leptohyphes ecuador, Baetodes levis and Prebaetodes sitesi. The association between nymphs and adults of L. albipennis and L. coconuco by this analysis confirmed the effectiveness of the technique. The molecular fingerprints of B. levis and P. sitesi were generated with the hope that future collections of unknown adults would match the fingerprint and solve an identification problem. We describe here for the first time the adult of L. ecuador by matching an adult trapped in the field with the DNA fingerprint of nymphs obtained by means of AFLP.

Keywords

EphemeropteranymphsadultsassociationDNAAFLPfingerprint
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 47 , Issue 2 , 2011 , pp. 151 - 157
Copyright
© EDP Sciences, 2011

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