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A molecular phylogeny of the benign Theileria parasites based on major piroplasm surface protein (MPSP) gene sequences

Published online by Cambridge University Press:  23 November 2009

W. JEONG ,
S. H. YOON ,
D. J. AN ,
S.-H. CHO ,
K.-K. LEE  and
J.-Y. KIM
W. JEONG
Affiliation:
Division of Veterinary Biotechnology, National Veterinary Research and Quarantine Service, 480, Anyang 6-dong, Anyang, Gyeonggido, 430-824South Korea
S. H. YOON
Affiliation:
Division of Veterinary Biotechnology, National Veterinary Research and Quarantine Service, 480, Anyang 6-dong, Anyang, Gyeonggido, 430-824South Korea
D. J. AN
Affiliation:
Division of Veterinary Biotechnology, National Veterinary Research and Quarantine Service, 480, Anyang 6-dong, Anyang, Gyeonggido, 430-824South Korea
S.-H. CHO
Affiliation:
Division of Malaria and Parasitic Disease, National Institute of Health, Tong-Il Lo 194, Ennpyeong-Gu, 122-701, Seoul, South Korea
K.-K. LEE
Affiliation:
Department of Veterinary Internal Medicine, Cheju University, Ara 1-dong, Jeju-si, Jeju-do, 690-756, South Korea
J.-Y. KIM*
Affiliation:
Division of Malaria and Parasitic Disease, National Institute of Health, Tong-Il Lo 194, Ennpyeong-Gu, 122-701, Seoul, South Korea
*
*Corresponding author: Division of Malaria and Parasitic Disease, National Institute of Health, Tong-Il Lo 194, Ennpyeong-Gu, 122-701, Seoul, Korea. Tel: +8223802183. Fax: +8223801560. E-mail: jykim-malaria@nih.go.kr
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Summary

To investigate the phylogeny of benign Theileria parasites, we determined the complete major piroplasm surface protein (MPSP) gene sequences for 6 benign theilerial organisms, including the first from tick. Sequences were analysed alongside published sequences for 39 benign Theileria parasites, using Bayesian inference and maximum parsimony. All MPSP sequences were 852 nucleotides, except for Gansu, Wuchangbuf, VB01, and VB01; Gansu contained 873 nucleotides, and the other 3 had 855. Deduced amino acid sequences contained 284 residues, except for Gansu (291) and Wuchangbuf, VB01, and VB01 (285 each). Pairwise comparisons showed identities among 45 theilerial MPSP sequences ranging from 70·9 to 99·8% for nucleotide and 71·0 to 100% for amino acid sequences. Our results clearly indicate that all global parasites, excluding Brisbane, were classified into 1 of 8 types; 6 types of Theileria exist in Korea. Each type, excluding Type 6, has several type-specific amino acid sequences. The phylogenetic tree derived from the nucleotide sequences showed 2 sister-group relationships, Type 2+Type 7 and Type 3+Brisbane, with a new branching pattern: (Type 6 (Type 8 ((Type 2, Type 7), (Type 1, (Type 4, (Type 5, (Type 3, Brisbane))))))). Our sequence data showed no geographical influence on worldwide Theileria parasite distribution.

Keywords

Theileriaphylogenymajor piroplasm surface proteingene sequences
Type
Research Article
Information
Parasitology , Volume 137 , Issue 2 , February 2010 , pp. 241 - 249
Copyright
Copyright © Cambridge University Press 2009

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