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The application of RAPD markers to the study of the bivalve mollusc Lasaea rubra

Published online by Cambridge University Press:  11 May 2009

H. Tyler-Walters  and
A. R. Hawkins
H. Tyler-Walters
Affiliation:
Department of Biochemistry and Genetics, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH
A. R. Hawkins
Affiliation:
Department of Biochemistry and Genetics, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH
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Extract

This paper describes the random amplification of polymorphic DNA markers (RAPDs) in Lasaea rubra (Erycinidae: Bivalvia). Present evidence suggests that L. rubra is an asexual species; however, the exact mode of clonal reproduction in this species is still a matter of debate. In this preliminary study, four of the primers used generated polymorphic RAPDs. One primer was able to distinguish between individuals from the same or different crevice population. This same primer also resolved a single band difference between otherwise identical RAPD patterns of a parent and its offspring. No familial differences have been detected in several previous studies using allozyme electrophoresis. This paper suggests that many polymorphic markers could be obtained with this species using the RAPD technique. Population genetic analysis of L. rubra has long been hampered by a dearth of polymorphic markers due to its small size. These findings suggest that this technique has the potential to further the study of population genetics in this asexual species.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 75 , Issue 3 , August 1995 , pp. 563 - 569
Copyright
Copyright © Marine Biological Association of the United Kingdom 1995

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