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The oxidation of lipids by components of bovine milk-fat globule membrane

Published online by Cambridge University Press:  01 June 2009

J. C. Allen  and
Catherine Humphries
J. C. Allen
Affiliation:
Department of Biochemistry, The Medical College of St Bartholomew's Hospital, London
Catherine Humphries
Affiliation:
Department of Biochemistry, The Medical College of St Bartholomew's Hospital, London
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Summary

Bovine milk-fat globule membrane was solubilized with a zwitterionic surfactant and subjected to chromatography on agarose, with the surfactant in the eluant. Fractions were tested for their effects on the oxidation of buffered linoleate. The maximum oxidative capability was greatly enhanced by the addition of Cu, and became associated with the phospholipids.

Further chromatography of the retarded protein peak from agarose on Sephadex G-200, again in the presence of surfactant, gave 2 protein peaks. Oxidative effectiveness resided almost entirely in the first peak, which was devoid of phospholipid, but high in xanthine oxidase activity. This fraction was subjected to isoelectric focusing, and the xanthine oxidase from this was highly pro-oxidative. Furthermore, its oxidative capability was almost doubled on heat treatment.

Type
Original Articles
Information
Journal of Dairy Research , Volume 44 , Issue 3 , October 1977 , pp. 495 - 507
Copyright
Copyright © Proprietors of Journal of Dairy Research 1977

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References

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