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The effect of docosahexaenoic acid and folic acid supplementation on placental apoptosis and proliferation

Published online by Cambridge University Press:  08 March 2007

Mario Klingler
Affiliation:
Dr von Hauner Children's Hospital, Ludwig-Maximilians-University of Munich, Munich, Germany Clinic of Obstetrics and Gynaecology, Medical University of Graz, Graz, Austria
Astrid Blaschitz
Affiliation:
Institute of Cell BiologyHistology and Embryology, Medical University of Graz, Graz, Austria
Christina Campoy
Affiliation:
Department of Paediatrics, School of Medicine, University of Granada, Granada, Spain
Ana Caño
Affiliation:
Department of Paediatrics, School of Medicine, University of Granada, Granada, Spain
Anne M Molloy
Affiliation:
Department of Clinical Medicine,Trinity College Dublin, Republic of Ireland
John M Scott
Affiliation:
Department of Clinical Medicine,Trinity College Dublin, Republic of Ireland
Gottfried Dohr
Affiliation:
Institute of Cell BiologyHistology and Embryology, Medical University of Graz, Graz, Austria
Hans Demmelmair
Affiliation:
Dr von Hauner Children's Hospital, Ludwig-Maximilians-University of Munich, Munich, Germany
Berthold Koletzko
Affiliation:
Dr von Hauner Children's Hospital, Ludwig-Maximilians-University of Munich, Munich, Germany
Gernot Desoye*
Affiliation:
Clinic of Obstetrics and Gynaecology, Medical University of Graz, Graz, Austria
*
*Corresponding author: Dr Gernot Desoye, fax +43 316 385 2506, email gernot.desoye@meduni-graz.at
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Abstract

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The hypothesis was tested that the additional dietary uptake of n-3 fatty acids, in particular of DHA and 5-methyltetrahydrofolate (5-MTHF), during the second half of pregnancy would influence proliferation and apoptosis in the full-term human placenta. The diets of pregnant women from Spain (n 55) were supplemented with modified fish oil and/or 5-MTHF or placebo, and assigned in a random, double-blind manner to one of the four groups. Immunohistochemistry and immunoblotting were used to detect placental proliferation and apoptosis with monoclonal antibodies for key proteins that reflected the extent of both processes: proliferation cell nuclear antigen (PCNA), p53, cytokeratin 18 neoepitope. The PCNA level in the fish oil/5-MTHF-treated group was higher by 66% (P<0·05) than that of the placebo group, whereas the levels of p53 and cytokeratin 18 neoepitope were unaffected by treatment. PCNA expression was altered only in the trophoblast compartment (placebo 11·1 (se 0·5) % V. combination 21·5 (se 1·2) % P<0·05), whereas the proportion of nuclei stained in endothelial and other stromal cells was similar in the placebo and combined treatment groups. No correlation was found between fish oil or 5-MTHF supplementation and the levels of the proteins. The present data suggest that supplementation with fish oil and/or 5-MTHF had no effect on the parameters reflecting placental proliferation and apoptosis. A defined combination of DHA and 5-MTHF may, however, affect placental proliferation.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2006

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