Hostname: page-component-7c8c6479df-nwzlb Total loading time: 0 Render date: 2024-03-26T17:41:14.271Z Has data issue: false hasContentIssue false

Incorporation of cis-9, trans-11 conjugated linoleic acid and vaccenic acid (trans-11 18:1) into plasma and leucocyte lipids in healthy men consuming dairy products naturally enriched in these fatty acids

Published online by Cambridge University Press:  08 March 2007

Graham C. Burdge*
Affiliation:
Institute of Human Nutrition, Biomedical Sciences Building, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
Sabine Tricon
Affiliation:
Hugh Sinclair Unit of Human Nutrition, University of Reading, Reading, UK
Rebecca Morgan
Affiliation:
Centre for Dairy Research, Department of Agriculture, University of Reading, Reading, UK
Kirsty E. Kliem
Affiliation:
Centre for Dairy Research, Department of Agriculture, University of Reading, Reading, UK
Caroline Childs
Affiliation:
Institute of Human Nutrition, Biomedical Sciences Building, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
Emma Jones
Affiliation:
Hugh Sinclair Unit of Human Nutrition, University of Reading, Reading, UK
Jennifer J. Russell
Affiliation:
Institute of Human Nutrition, Biomedical Sciences Building, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
Robert F. Grimble
Affiliation:
Institute of Human Nutrition, Biomedical Sciences Building, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
Christine M. Williams
Affiliation:
Hugh Sinclair Unit of Human Nutrition, University of Reading, Reading, UK
Parveen Yaqoob
Affiliation:
Hugh Sinclair Unit of Human Nutrition, University of Reading, Reading, UK
Philip C. Calder
Affiliation:
Institute of Human Nutrition, Biomedical Sciences Building, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK
*
*Corresponding author: Dr Graham C. Burdge, fax +44 2380 594379, email g.c.burdge@soton.ac.uk
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The present study investigated whether consuming dairy products naturally enriched in cis-9, trans-11 (c9, t11) conjugated linoleic acid (CLA) by modification of cattle feed increases the concentration of this isomer in plasma and cellular lipids in healthy men. The study had a double-blind cross-over design. Subjects aged 34–60 years consumed dairy products available from food retailers for 1 week and then either control (0·17 g c9, t11 CLA/d; 0·31 g trans-vaccenic acid (tVA)/d) or CLA-enriched (1·43 g c9, t11 CLA/d; 4·71 g tVA/d) dairy products for 6 weeks. After 7 weeks washout, this was repeated with the alternate products. c9, t11 CLA concentration in plasma lipids was lower after consuming the control products, which may reflect the two-fold greater c9, t11 CLA content of the commercial products. Consuming the CLA-enriched dairy products increased the c9, t11 CLA concentration in plasma phosphatidylcholine (PC) (38%; P=0·035), triacylglycerol (TAG) (22%; P<0·0001) and cholesteryl esters (205%; P<0·0001), and in peripheral blood mononuclear cells (PBMC) (238%; P<0·0001), while tVA concentration was greater in plasma PC (65%; P=0·035), TAG (98%; P=0·001) and PBMC (84%; P=0·004). Overall, the present study shows that consumption of naturally enriched dairy products in amounts similar to habitual intakes of these foods increased the c9, t11 CLA content of plasma and cellular lipids.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2005

References

Alasnier, C, Berdeaux, O, Chardigny, JM & Sebedio, JL (2002) Fatty acid composition and conjugated linoleic acid content of different tissues in rats fed individual conjugated linoleic acid isomers given as triacylglycerols. J Nutr Biochem 13, 337345.CrossRefGoogle ScholarPubMed
Albers, R, van der Wielen, RP, Brink, EJ, Hendriks, HF, Dorovska-Taran, VN & Mohede, IC (2003) Effects of cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid (CLA) isomers on immune function in healthy men. Eur J Clin Nutr 57, 595603.CrossRefGoogle ScholarPubMed
Banni, S, Carta, G, Angioni, E, Murru, E, Scanu, P, Melis, MP, Bauman, DE, Fischer, SM & Ip, C (2001) Distribution of conjugated linoleic acid and metabolites in 13 different lipid fractions in the rat liver. J Lipid Res 42, 10561061.CrossRefGoogle Scholar
Belury, MA (2002) Inhibition of carcinogenesis by conjugated linoleic acid: potential mechanisms of action. J Nutr 132, 29952998.CrossRefGoogle ScholarPubMed
Benito, P, Nelson, GJ, Kelley, DS, Bartolini, G, Schmidt, PC & Simon, V (2001) The effect of conjugated linoleic acid on plasma lipoproteins and tissue fatty acid composition in humans. Lipids 36, 229236.CrossRefGoogle ScholarPubMed
Burdge, GC, Lupoli, B, Russell, JJ, et al. (2004) Incorporation of cis-9, trans-11 or trans-10, cis-12 conjugated linoleic acid into plasma and cellular lipids in healthy men. J Lipid Res 45, 736741.CrossRefGoogle ScholarPubMed
Burdge, GC, Wright, P, Jones, AE & Wootton, SA (2000) A method for separation of phosphatidylcholine, triacylglycerol, non-esterified fatty acids and cholesterol esters from plasma by solid-phase extraction. Br J Nutr 84, 781787.CrossRefGoogle ScholarPubMed
Calder, PC (2002) Conjugated linoleic acid in humans - reasons to be cheerful? Curr Opin Clin Nutr Metab Care 5, 123126.CrossRefGoogle ScholarPubMed
Chardigny, JM, Masson, E, Sergiel, JP, Darbois, M, Loreau, O, Noel, JP & Sebedio, JL (2003) The position of rumenic acid on triacylglycerols alters its bioavailability in rats. J Lipid Res 133, 42124214.Google ScholarPubMed
Christie, WW (1982) A simple procedure for rapid transmethylation of glycerolipids and cholesteryl esters. J Lipid Res 23, 10721075.CrossRefGoogle ScholarPubMed
DeLany, JP, Blohm, F, Truett, AA, Scimeca, JA & West, DB (1999) Conjugated linoleic acid rapidly reduces body fat content in mice without affecting energy intake. Am J Physiol 276, R1172R1179.Google ScholarPubMed
Folch, JL, Lees, M & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226, 497509.CrossRefGoogle ScholarPubMed
Griinari, JM, Dwyer, DA, McGuire, MA, Bauman, DE, Palmquist, DL & Nurmela, KVV (1998) Trans-octadecenoic acids and milk fat depression in lactating dairy cows. J Dairy Sci 81, 12511261.CrossRefGoogle ScholarPubMed
Ip, C, Chin, SF, Scimeca, JA & Pariza, MW (1991) Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer Res 51, 61186124.Google ScholarPubMed
Jones, EL, Shingfield, KJ, Kohen, C, Jones, AK, Lupoli, B, Grandison, AS, Beever, DE, Williams, CM, Calder, PC & Yaqoob, P (2005) Chemical, physical and sensory properties of diary products enriched with conjugated linoleic acid. J Dairy Sci (In the Press).CrossRefGoogle Scholar
Kelley, DS & Erickson, KL (2003) Modulation of body composition and immune cell functions by conjugated linoleic acid in humans and animal models: benefits vs. risks. Lipids 38, 377386.CrossRefGoogle ScholarPubMed
Kelley, DS, Simon, VA, Taylor, PC, Rudolph, IL, Benito, P, Nelson, GJ, Mackey, BE & Erickson, KL (2001) Dietary supplementation with conjugated linoleic acid increased its concentration in human peripheral blood mononuclear cells, but did not alter their function. Lipids 36, 669674.CrossRefGoogle Scholar
Kew, S, Banerjee, T, Minihane, AM, Finnegan, YE, Williams, CM & Calder, PC (2003) Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in healthy, free-living subjects aged 25–72 y. Am J Clin Nutr 77, 12781286.CrossRefGoogle ScholarPubMed
Lawson, RE, Moss, AR & Givens, DI (2001) The role of dairy products in supplying conjugated linoleic acid to man's diet: a review. Nutr Res 14, 153172.CrossRefGoogle ScholarPubMed
Lee, KN, Kritchevsky, D & Pariza, MW (1994) Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108, 1925.CrossRefGoogle ScholarPubMed
Malpuech-Brugere, C, Verboeket-Van de Venne, WP, Mensink, RP, et al. (2004) Effects of two conjugated linoleic acid isomers on body fat mass in overweight humans. Obes Res 12, 591598.CrossRefGoogle ScholarPubMed
Masters, N, McGuire, MA, Beerman, KA, Dasgupta, N & McGuire, MK (2002) Maternal supplementation with CLA decreases milk fat in humans. Lipids 37, 133138.CrossRefGoogle ScholarPubMed
Mougios, V, Matsakas, A, Petridou, A, Ring, S, Sagredos, A, Melissopoulou, A, Tsigilis, N & Nikolaidis, M (2001) Effect of supplementation with conjugated linoleic acid on human serum lipids and body fat. J Nutr Biochem 12, 585594.CrossRefGoogle ScholarPubMed
Munday, JS, Thompson, KG & James, KA (1999) Dietary conjugated linoleic acids promote fatty streak formation in the C57BL/6 mouse atherosclerosis model. Br J Nutr 81, 251255.CrossRefGoogle ScholarPubMed
Nicolosi, RJ, Rogers, EJ, Kritchevsky, D, Scimeca, JA & Huth, PJ (1997) Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery 22, 266277.Google ScholarPubMed
Noone, EJ, Roche, HM, Nugent, AP & Gibney, MJ (2002) The effect of dietary supplementation using isomeric blends of conjugated linoleic acid on lipid metabolism in healthy human subjects. Br J Nutr 88, 243251.CrossRefGoogle ScholarPubMed
Park, Y, Albright, KJ, Liu, W, Storkson, JM, Cook, ME & Pariza, MW (1997) Effect of conjugated linoleic acid on body composition in mice. Lipids 32, 853858.CrossRefGoogle ScholarPubMed
Petridou, A, Mougios, V & Sagredos, A (2003) Supplementation with CLA: isomer incorporation in serum lipids and effect on body fat of women. Lipids 38, 805811.CrossRefGoogle ScholarPubMed
Roche, HM, Noone, E, Nugent, A & Gibney, MJ (2001) Conjugated linoleic acid: a novel therapeutic nutrient? Nutr Res 14, 173187.CrossRefGoogle ScholarPubMed
Sebedio, JL, Angioni, E, Chardigny, JM, Gregoire, S, Juaneda, P & Berdeaux, O (2001) The effect of conjugated linoleic acid isomers on fatty acid profiles of liver and adipose tissues and their conversion to isomers of 16:2 and 18:3 conjugated fatty acids in rats. Lipids 36, 575582.CrossRefGoogle Scholar
Shingfield, KJ, Ahvenjarvi, S, Toivonen, V, Arola, A, Nurmela, KVV, Hutanen, P & Criinari, JM (2003) Effect of dietary fish oil on biohydrogenation of fatty acids and milk fatty acid content in cows. Anim Sci 77, 165179.CrossRefGoogle Scholar
Tricon, S, Burdge, GC, Kew, S, Banerjee, T, Russell, JJ, Grimble, RF, Williams, CM, Calder, PC & Yaqoob, P (2004 a) Effects of cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid on immune cell function in healthy humans. Am J Clin Nutr 80, 16261633.CrossRefGoogle ScholarPubMed
Tricon, S, Burdge, GC, Kew, S, Banerjee, T, Russell, JJ, Jones, EL, Grimble, RF, Williams, CM, Yaqoob, P & Calder, PC (2004 b) Opposing effects of cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid on blood lipids in healthy humans. Am J Clin Nutr 80, 614620.CrossRefGoogle ScholarPubMed
Turpeinen, AM, Mutanen, M, Aro, A, Salminen, I, Basu, S, Palmquist, DL & Griinari, JM (2002) Bioconversion of vaccenic acid to conjugated linoleic acid in humans. Am J Clin Nutr 76, 504510.CrossRefGoogle ScholarPubMed
Von Loeffelholz, C, Kratzsch, J & Jahreis, G (2003) Influence of conjugated linoleic acids on body composition and selected serum and endocrine parameters in resistance-trained athletes. Eur J Lipid Sci Technol 105, 251259.CrossRefGoogle Scholar
Watkins, BA, Li, Y, Lippman, HE, Reinwald, S & Seifert, MF (2004) A test of Ockham's razor: implications of conjugated linoleic acid in bone biology. Am J Clin Nutr 79, Suppl., 1175S1185S.CrossRefGoogle ScholarPubMed
West, DB, Delany, JP, Camet, PM, Blohm, F, Truett, AA & Scimeca, J (1998) Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am J Physiol 275, R667R672.Google ScholarPubMed
Zock, PL & Katan, MB (1992) Hydrogenation alternatives: effects of trans fatty acids and stearic acid versus linoleic acid on serum lipids and lipoproteins in humans. J Lipid Res 33, 399410.CrossRefGoogle ScholarPubMed