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Ontogeny and nutritional manipulation of mitochondrial protein abundance in adipose tissue and the lungs of postnatal sheep

Published online by Cambridge University Press:  09 March 2007

A. Mostyn ,
V. Wilson ,
J. Dandrea ,
D. P. Yakubu ,
H. Budge ,
M. C. Alves-Guerra ,
C. Pecqueur ,
B. Miroux ,
M. E. Symonds  and
T. Stephenson
A. Mostyn
Affiliation:
Academic Division of Child Health, School of Human Development, Queen's Medical Centre, University Hospital, Nottingham NG7 2UH, UK
V. Wilson
Affiliation:
Academic Division of Child Health, School of Human Development, Queen's Medical Centre, University Hospital, Nottingham NG7 2UH, UK
J. Dandrea
Affiliation:
Academic Division of Child Health, School of Human Development, Queen's Medical Centre, University Hospital, Nottingham NG7 2UH, UK
D. P. Yakubu
Affiliation:
Academic Division of Child Health, School of Human Development, Queen's Medical Centre, University Hospital, Nottingham NG7 2UH, UK
H. Budge
Affiliation:
Academic Division of Child Health, School of Human Development, Queen's Medical Centre, University Hospital, Nottingham NG7 2UH, UK
M. C. Alves-Guerra
Affiliation:
CNRS-CEREMOD, 9 rue Jules Hetzel, 92190 Meudon, France
C. Pecqueur
Affiliation:
CNRS-CEREMOD, 9 rue Jules Hetzel, 92190 Meudon, France
B. Miroux
Affiliation:
CNRS-CEREMOD, 9 rue Jules Hetzel, 92190 Meudon, France
M. E. Symonds*
Affiliation:
Academic Division of Child Health, School of Human Development, Queen's Medical Centre, University Hospital, Nottingham NG7 2UH, UK
T. Stephenson
Affiliation:
Academic Division of Child Health, School of Human Development, Queen's Medical Centre, University Hospital, Nottingham NG7 2UH, UK
*
*Corresponding author: Dr Michael E. Symonds, fax +44 115 970 9382, email Michael.Symonds@nottingham.ac.uk
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Abstract

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The present study examined the ontogeny of mitochondrial protein abundance in adipose tissue and lungs over the first month of life in the sheep and the extent to which this may be altered by maternal undernutrition during the final month of gestation. The ontogeny of uncoupling protein (UCP), voltage-dependent anion channel (VDAC) and cytochrome c abundance were determined in adipose tissue and lungs sampled from near-term fetuses and young sheep aged 4 h, 1, 7 and 30 d. In adipose tissue, the abundance of UCP1, VDAC and cytochrome c all peaked at 1 d of age and then decreased by 30 d of age, at which stage the brown adipose tissue-specific UCP1 was no longer detectable but UCP2 was clearly abundant. For the lungs, however, UCP2 and VDAC abundance both peaked 7 d after birth and then decreased by 30 d of age. During postnatal development, therefore, a marked change in mitochondrial protein abundance occurs within both adipose tissue and lungs. Maternal nutrient restriction had no effect on lamb growth or tissue weights at 30 d of age but was associated with increased abundance of UCP2 and VDAC but not cytochrome c in both adipose tissue and lungs. These mitochondrial adaptations within both adipose tissue and the lungs of offspring born to previously nutrient-restricted mothers may compromise adipose tissue and lung function during periods of environmental stress.

Keywords

Adipose tissueDevelopmentLungs mitochondriaProteins
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 2 , August 2003 , pp. 323 - 328
Copyright
Copyright © The Nutrition Society 2003

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