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A Japanese diet and 19-year mortality: National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980

Published online by Cambridge University Press:  20 November 2008

Yasuyuki Nakamura*
Affiliation:
Cardiovascular Epidemiology, Kyoto Women's University, 35 Imakumano Kitahiyoshi-cho, Higashiyama-ku, Kyoto605-8501, Japan Department of Health Science, Shiga University of Medical Science, Otsu City, Shiga, Japan
Hirotsugu Ueshima
Affiliation:
Department of Health Science, Shiga University of Medical Science, Otsu City, Shiga, Japan
Tomonori Okamura
Affiliation:
Department of Health Science, Shiga University of Medical Science, Otsu City, Shiga, Japan
Takashi Kadowaki
Affiliation:
Department of Health Science, Shiga University of Medical Science, Otsu City, Shiga, Japan
Takehito Hayakawa
Affiliation:
Department of Hygiene and Preventive Medicine, Fukushima Medical University, Fukushima, Japan
Yoshikuni Kita
Affiliation:
Department of Health Science, Shiga University of Medical Science, Otsu City, Shiga, Japan
Robert D. Abbott
Affiliation:
Department of Health Science, Shiga University of Medical Science, Otsu City, Shiga, Japan Department of Epidemiology and Biostatistics, University of Virginia School of Medicine, Charlottesville, VA, USA
Akira Okayama
Affiliation:
Department of Preventive Cardiology, National Cardiovascular Center, Suita City, Japan
*
*Corresponding author: Yasuyuki Nakamura, fax +81 75 531 2162, email nakamury@kyoto-wu.ac.jp
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Abstract

Few studies have examined the association between Japanese diet and mortality outcomes. We analysed the relationship between a healthy Japanese diet and all-cause and cause-specific mortality using the database from the National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980. At baseline in 1980, data were collected on study participants aged ≧30 years from randomly selected areas in Japan. We defined a measure of a healthy reduced-salt Japanese diet based on seven components from FFQ. The total score ranged from 0 to 7, with 0 being least healthy and 7 being most healthy. Participants were divided into approximate tertiles of dietary scores (0–2, 3 and 4–7 scores). After excluding participants with co-morbidities, we followed 9086 participants (44 % men) for 19 years. There were 1823 all-cause and 654 cardiovascular deaths during the follow-up. With the dietary score group 0–2 serving as a reference, the Cox multivariate-adjusted hazard ratios for groups with scores 3 and 4–7 were 0·92 (95 % CI 0·83, 1·04) and 0·78 (95 % CI 0·70, 0·88) for all-cause mortality (trend P < 0·0001), and 0·91 (95 % CI 0·75, 1·10) and 0·80 (95 % CI 0·66, 0·97) for cardiovascular mortality (trend P = 0·022). Adherence to a healthy reduced-salt Japanese diet was associated with an approximate 20 % lower rate of all-cause and cardiovascular mortality.

Type
Full Papers
Copyright
Copyright © The Authors 2008

Recent interest in dietary patterns has spawned several studies of the associations between dietary patterns and longevity(Reference Huijbregts, Feskens and Rasanen1, Reference Trichopoulou, Costacou and Bamia2). Japanese cuisine is based on combining staple foods, typically rice or noodles, with soup, and side dishes made from fish, meat, vegetable, tofu and the like, designed to add flavour to the staple food. These are typically flavoured with dashi stock, made with katsuobushi (dried skipjack tuna flakes), miso and soya sauce, and are usually low in fat and high in salt. Since Japan is an island nation, people eat much seafood. Meat eating has been relatively rare. The beneficial aspects of the traditional Japanese diet have been attributed to its low intake of SFA and a high intake of PUFA, especially from fish. Long-term benefits include lower mortality from CHD and from some cancers, which contribute at least in part to Japanese having the longest life expectancy in the world(Reference Robertson, Kato and Gordon3, Reference Carroll4). A drawback of the Japanese diet is its high intake of salt and its association with a higher incidence and mortality from stroke and gastric cancer(Reference Ueshima, Zhang and Choudhury5Reference Nagata, Takatsuka and Shimizu7). Presumably, if the Japanese diet is modified to emphasise the intake of foods that are low in salt, Japanese longevity could be increased further.

In the present study, we studied the preference for Japanese or Western diets, and from these data and those based on the previous studies, we comprehensively extracted the beneficial components of the Japanese diet and derived a healthy Reduced-Salt Japanese Diet Score. We analysed the relationship between the diet score and all-cause and cause-specific mortality using the database of the National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980 (NIPPON DATA80). The database includes more than 10 000 participants from randomly selected regions in Japan, who were followed for 19 years(Reference Okamura, Kadowaki and Hayakawa8Reference Nakamura, Ueshima and Okamura10).

Methods

Participants

The participants in this cohort were those in the 1980 National Survey on Circulatory Disorders(Reference Okamura, Kadowaki and Hayakawa8). A total of 10 546 community-based participants aged 30 years and above in 300 randomly selected health districts throughout Japan participated in the survey, which consisted of history taking, physical examinations, blood tests and a self-administered questionnaire on lifestyle, including an essential nutritional survey by the food-frequency method. For the present study, the participants were followed up to 1999 (National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980, 1980–99). The overall population aged 30 years and above in the participating health districts was 13 771. The participation rate was 76·6 % (10 546 of 13 771) before exclusion for reasons mentioned later.

We reviewed the residence records of all the study participants for their vital status. In the cases of deaths, the causes were examined. To clarify the cause of death, we used the National Vital Statistics records. The underlying cause of death was coded according to the ninth International Classification of Disease for the National Vital Statistics until the end of 1994 and according to the tenth International Classification of Disease from the beginning of 1995. Deaths were confirmed in each district by computer matching of data from the National Vital Statistics records using the district, sex and dates of birth and death as key codes.

Participants were excluded from follow-up because of a past history of coronary disease, stroke or significant co-morbidities such as renal insufficiency (n 539), because of missing baseline data (n 51) or because of a loss to follow-up (n 870). The latter group was excluded because of the absence of a permanent address that was required for linking to National Vital Statistics records. The final sample comprised 9086 participants (4018 men and 5068 women). There were no significant differences between participants who were lost to follow-up and those who were included in the present study in terms of several risk factor characteristics. Therefore, the potential bias regarding the 870 participants lost to follow-up is thought to be negligible. Permission to use the National Vital Statistics records was obtained from the Management and Coordination Agency, Government of Japan. Approval for the present study was obtained from the Institutional Review Board of Shiga University of Medical Science for ethical issues (no. 12–18, 2000).

Biochemical and baseline examinations

The baseline surveys were conducted at public health centres. Baseline blood pressures (BP) were measured by trained research nurses using a standard mercury sphygmomanometer on the right arm of seated participants after at least 5 min of rest. Hypertension was defined as systolic BP ≥ 140 mmHg, diastolic BP ≥ 90 mmHg, or when a participant was receiving medications for the treatment of high BP. Height and weight were measured in stocking feet and light clothing. BMI was calculated as weight (kg) divided by the square of height (m2).

A lifestyle survey was also carried out using a self-administered questionnaire that asked about the typical daily consumption of thirty-one food items, as shown in Appendix. Egg consumption was coded as ≥ 2 eggs/d, about 1 egg/d, about 1 egg/2 d, about 1–2 eggs/week and less than once per week. Fish, meat and tsukemono (preserved roots or leaves of seasonal vegetables, e.g. cucumbers and aubergine, which are consumed with rice at the end of a meal) intake was coded separately as ≥ 2 times/d, about 1 time/d, about 1 time/2 d, about 1–2 times/week and less than once per week. The participants were also asked whether they frequently consumed soup with noodles, whether they used low-salt soya sauce and what their preferred type of diet was (Japanese, Western or mixed; Q19 in Appendix). They were enquired about their alcohol drinking habit (never, past, occasional and daily drinkers). Reported information was confirmed by public health nurses through interviews with the study participants regarding food consumption, smoking, drinking habit and present and past medical histories.

Non-fasting blood samples were drawn and centrifuged within 60 min of collection and stored at − 70°C until analyses. Serum total cholesterol, albumin, uric acid and creatinine were analysed in a sequential auto-analyser (SMA12/60, Technicon, Tarrytown, NY, USA) at a single laboratory (Osaka Medical Center for Health Science and Promotion). This laboratory is a member of the Cholesterol Reference Method Laboratory Network(Reference Nakamura, Sato and Shimamoto11). Serum concentrations of glucose were measured by the cupric-neocuproine method(Reference Kromhout, Bosschieter and de Lezenne Coulander12). Diabetes was determined by medical history or defined as a serum glucose concentration ≥ 2000 mg/l.

Statistical analysis and components of the Reduced-Salt Japanese Diet Score

Statistical Analysis Systems statistical software package version 9.1 for Windows (SAS Institute, Cary, NC, USA) was used throughout the analyses. We examined the relationship between the type of preferred diet and the frequency of dietary components from the nutritional survey. Then, we defined seven components from the nutritional survey to measure a healthy reduced-salt Japanese diet. The components included egg intake ≤ 2 eggs/week, fish intake once or more often in 2 d, meat intake ≤ 2 times/week, tsukemono intake once or more often per day, infrequent intake of soup with noodles, use of low-salt soya sauce and occasional drinking. The afore-mentioned cut-off values were determined based on the previous studies on the intake of eggs, fish and alcohol(Reference Nakamura, Okamura and Tamaki9, Reference Nakamura, Ueshima and Okamura10, Reference Daviglus, Stamler and Orencia13Reference Tsugane, Fahey and Sasaki15). For meat and tsukemono, a near median was used as the cut-off. Infrequent intake of soup with noodles and the use of low-salt soya sauce were used as markers of salt restriction. Because data on the amounts of alcohol consumed were not available, and the association between all-cause mortality and alcohol consumption is known to be J-shaped(Reference Tsugane, Fahey and Sasaki15), we chose occasional drinking as a component of a healthy reduced-salt Japanese diet. Moderate alcohol consumption was also a component of a Mediterranean diet(Reference Trichopoulou, Costacou and Bamia2). If any single dietary component was part of a typical daily diet, it was scored as 1 and 0 otherwise. Thus, the total score ranged from 0 to 7, with 0 being least healthy and 7 being most healthy. The participants were divided into approximate tertiles of dietary scores (0–2, 3 and 4–7 scores). To obtain trend P, the Mantel–Haenszel χ2 statistical test was used to detect deviation from linearity in the association between nominal variables and the categories according to the diet score, and the ANOVA was used to detect deviation from linearity in the association between continuous variables and the categories. To examine the association between the Reduced-Salt Japanese Diet Score and all-cause and cause-specific mortality, age -, sex- and multivariate-adjusted hazard ratios were calculated using a Cox proportional hazards model. For multivariate analyses, age, sex, BMI and cigarette smoking (never and past smokers, current smokers < 20 cigarettes/d, current smokers 20–40 cigarettes/d and current smokers ≥ 41 cigarettes/d) were entered as covariates for model 1. For model 2, hypertension and diabetes were added. The dietary score group 0–2 served as a reference for comparison with the other tertiles. Sensitivity analyses were performed on the afore-mentioned Cox analysis by excluding those who did not report a preferred food type, by stratifying the lower and higher age groups at median age, 49·3 years, and by stratifying by sex. To examine the association between each of the components of a Reduced-Salt Japanese Diet Score and all-cause mortality, adjustments were made for the covariates in model 2.

To estimate adjusted survival probabilities, we derived Kaplan–Meier survival curves after propensity score matching(Reference Parsons16). Variables used in the propensity score were selected from the non-dietary variables: age (years), men (%), BMI (kg/m2), current smokers (%), systolic BP (mmHg), diastolic BP (mmHg), on hypertension drugs (%), diabetes (%), serum total cholesterol (mg/l), albumin (mg/l), uric acid (mg/l) and creatinine (mg/l). After matching, adjusted survival curves were estimated separately for those participants who fell in the Japanese dietary grouping that ranged from 0 to 3 and for those in grouping strata 4 and higher. Comparison of the survival curves was based on the log-rank test. We further examined survival differences by the two groups according to the diet score, with age and sex as the dependent variables in a regression model. The statistical model used was a life table regression procedure, with a Weibull distribution assumption for failure time included. The variables used in the calculation of the propensity score were also compared by t test and χ2 test to determine whether the propensity score matching was successful in mitigating risk factor differences.

Results

Baseline characteristics and all-cause mortality according to preferred food type

The baseline characteristics according to the preferred food type are shown in Table 1. In this table, we excluded 201 participants with missing data on a preferred food type. Relatively few participants preferred the Western food type. Participants in this group were younger, were more likely to be women and were less often hypertensive than participants who chose the other diet types. Those who preferred a Western type of diet ate meat more frequently and consumed fish and tsukemono less often than those in the other groups. The two markers of salt restriction (infrequent consumption of soup with noodles and the use of low-salt soya sauce) were more prevalent among those who preferred a Western diet. Small differences, but a significant trend in the Reduced-Salt Japanese Diet Score, were observed (trend P < 0·0001).

Table 1 Baseline characteristics according to preferred food type – National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980, 1980–99*

(Mean values and standard deviations)

*We excluded 201 participants in this table, who did not choose their preferred food type. To obtain trend Ps, the Mantel–Haenszel χ2 statistical test was used for nominal variables, and the ANOVA for continuous variables.

Baseline characteristics according to Reduced-Salt Japanese Diet Score

Table 2 shows the baseline characteristics according to tertiles of the Reduced-Salt Japanese Diet Score. As the score increased, the mean age and BMI increased, although the latter increase was modest. The proportion of women and the prevalence of hypertension, daily drinking and non-smoking also increased with diet score. The prevalence of diabetes and the mean serum total cholesterol concentration were not significantly different across the groups. As expected, the percentage with each component of the Reduced-Salt Japanese Diet Score increased as the score increased.

Table 2 Baseline characteristics according to tertiles of the Reduced-Salt Japanese Diet Score among 4018 men and 5068 women – National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980, 1980–99*

(Mean values and standard deviations)

No., number; TCH, serum total cholesterol concentration.

*We defined a healthy Japanese diet based on seven components: egg intake ≤ 2 eggs/week, fish intake once or more often in 2 d, meat intake ≤ 2 times/week, tsukemono (preserved roots or leaves of seasonal vegetables) intake once or more often per day, infrequent intake of soup with noodles, use of low-salt soya sauce and occasional drinking. If a dietary component was part of a typical daily diet, it was scored as 1 and 0 otherwise. Thus, the total Reduced-Salt Japanese Diet Score ranged from 0 to 7, with 0 being least healthy and 7 being most healthy. To obtain trend Ps, the Mantel–Haenszel χ2 statistical test was used for nominal variables, and the ANOVA for continuous variables.

All-cause and cause-specific mortality according to Reduced-Salt Japanese Diet Score

During the 19 years of follow-up, there were 1823 deaths. In this group, 654 were from CVD, 299 from stroke, 131 acute myocardial infarction, 551 cancer and 119 non-cardiovascular, non-cancer inflammatory diseases(Reference Jacobs, Andersen and Blomhoff17). Table 3 shows the total person-years, numbers of cases, hazard ratios and 95 % CI for all-cause and cause-specific mortality for each category of Reduced-Salt Japanese Diet Score after adjustment for age, sex and other risk factors (multivariate models 1 and 2). As the score increased, risk of death from all-cause mortality, CVD and stroke declined significantly in all models. Mortality from acute myocardial infarction, cancer and inflammatory diseases tended to decrease, but without statistical significance, a possible consequence of the relatively small number of such events. Similar results were observed after excluding participants with missing data on dietary preference. At high-age strata and in men, similar results were observed for all-cause mortality, CVD and stroke mortality. However, at low-age strata and in women, results were similar for all-cause mortality only. Significant differences by the groups according to the diet score were lost at low-age strata and in women for CVD and stroke mortality, probably because of the relatively smaller number of such events at low-age strata.

Table 3 All-cause and cause-specific mortality according to Reduced-Salt Japanese Diet Score among 9089 men and women – National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980, 1980–99*

(Hazard ratios (HR) and 95 % confidence intervals)

AMI, acute myocardial infarction; Inflam., inflammatory disease.

*Multivariate, multivariate-adjusted Cox analysis. Model 1: adjusted for age, sex, BMI and smoking (never and ex-smokers, current smokers < 20 cigarettes/d, current smokers 20–40 cigarettes/d and current smokers ≥ 41 cigarettes/d). Model 2: adjusted for model 1 covariates plus hypertension and diabetes.

Components of Reduced-Salt Japanese Diet Score and all-cause mortality

The percentage of total participants who observed a healthy component of the Reduced-Salt Japanese Diet Score and the association of each component with all-cause mortality are shown in Table 4. The percentage of male participants who observed a healthy reduced-salt Japanese dietary component is also provided. Adherence to each of the healthy dietary components tended to be associated with lower mortality. Risk of death, however, was significantly lower for participants who ate tsukemono once or more often per day, consumed soup with noodles infrequently and drank alcohol occasionally.

Table 4 Components of Reduced-Salt Japanese Diet Score and all-cause mortality among 9089 men and women – National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980, 1980–99*

(Hazard ratios (HR) and 95 % confidence intervals)

Total (%), percentage of total participants who had each component of Reduced-Salt Japanese Diet Score; men %, percentage of men who had each component.

*Multivariate, multivariate-adjusted Cox analysis adjusted for age, sex, BMI, hypertension, diabetes and smoking (never and ex-smokers, current smokers < 20 cigarettes/d, current smokers 20–40 cigarettes/d and >40 cigarettes/d).

Kaplan-Meier survival estimates after propensity score matching

The results from the propensity score matching are shown in Table 5. Fifty-eight participants with the Reduced-Salt Japanese Diet Score 4–7 were unmatched due to missing data (n 56) or failure to match on propensity scores (n 2). As can be seen, significant differences in the average propensity score and the variables used in its calculation before matching in the two groups disappeared after matching. By contrast, a significant difference between the matched survival curves remained as shown in Fig. 1 (P = 0·0003 by log-rank test). Survival differences by the group were significant when examined further using a regression model with a Weibull distribution that included adjustment for age and sex as the dependent variables (estimate = − 0·13 (the lower score group compared with the higher score group), P < 0·0001).

Table 5 Variables used for propensity score matching and survival rate – National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980, 1980–99

(Mean values and standard deviations)

BP, blood pressure; TCH, serum total cholesterol concentration.

Fig. 1 Kaplan–Meier survival curve after propensity score matching. Significant differences in the average propensity score and the variables used in its calculation before matching in the two groups disappeared after matching. By contrast, a significant difference between the matched survival curves remained as shown in the figure (P = 0·0003 by log-rank test). Survival differences by the group were significant when examined further using a regression model with a Weibull distribution that included adjustment for age and sex as the dependent variables (P < 0·0001). The thick line indicates survival for the participants with the Reduced-Salt Japanese Diet Score 4–7 and the thin line with the Reduced-Salt Japanese Diet Score 0–3.

Discussion

The cut-off values for the egg, fish and drinking components were determined based on the previous studies(Reference Nakamura, Sato and Shimamoto11, Reference Kromhout, Bosschieter and de Lezenne Coulander12, Reference Ueshima, Iida and Shimamoto18Reference Tsugane, Sasazuki and Kobayashi23). Near-median cut-off values were used for meat and tsukemono. The low intake of meat is one of the characteristic features of the traditional Japanese diet and serves as a marker of reduced intake of SFA in the Japanese(Reference Robertson, Kato and Gordon3, Reference Carroll4, Reference Ueshima, Iida and Shimamoto18, Reference Ueshima, Okayama and Saitoh19). Although frequent intake of tsukemono is also a characteristic feature of the traditional Japanese diet, it was unexpected to find that consuming tsukemono at least once a day was associated with a statistically significant lower risk of all-cause mortality. Many types of Japanese tsukemono are prepared in a traditional Japanese fashion with high reliance on salt. It may be, however, that the more healthy nutrient content of tsukemono outweighs the adverse consequences from consuming tsukemono with high sodium content. Conversely, the healthy nutritional value from eating unsalted tsukemono may be modest and offer little prognostic significance. Rather, its association with lower mortality may be through a high likelihood of being associated with a traditional Japanese diet. Those who eat tsukemono may consume meat less often and prefer foods that are commonly enjoyed with tsukemono, such as fish, vegetables, fruits and soya bean products.

We merely do not eat foods, but in certain patterns(Reference Jacques and Tucker20), such as those in the Mediterranean and Japanese dietary patterns. Because of highly interrelated dietary exposures, dietary patterns, rather than the specific effects of nutrients or foods, have gained increasing attention(Reference Huijbregts, Feskens and Rasanen1, Reference Trichopoulou, Costacou and Bamia2). Although one drawback of the traditional Japanese diet is a high intake of salt, reduction in salt intake by the Japanese for the last three decades has been considered as one of the chief explanations for the decline in not only stroke but also stomach cancer mortality in Japan(Reference Ueshima, Zhang and Choudhury5, Reference Kubo, Kiyohara and Kato6, Reference Ueshima, Tatara and Asakura21, Reference Tsugane, Sasazuki and Kobayashi23). This is consistent with the finding in the present report that infrequent consumption of soups with noodles, a marker of low salt intake, was associated with a significantly lower risk of all-cause mortality by itself.

Strengths and limitations of the study

The strengths of the present study include its prospective design and the follow-up of a randomly selected sample from the general population of Japan with a high response rate (76 %). Since the study includes both men and women with a broad range of ages, findings are likely to be generalisable to middle-aged and elderly Japanese men and women.

As in any long-term follow-up study, however, there are several weaknesses. First, we surveyed essential nutritional components by the food-frequency method once at the baseline. As a result, we have no data on total caloric intake or total dietary intake of cholesterol or saturated and PUFA. To obtain these data, detailed food records or 24-h recalls are needed. However, these methods are impractical and seldom used as the primary method for estimating usual intake in large-scale epidemiological studies. A second limitation is that the items used for the food-frequency method were not large in number, and has not been validated. We do not have data to what extent these foods contribute to the average energy intake of the studied participants. We also do not have frequency data on tofu, other soya bean products and vegetables and fruits. A high intake of these foods may also be characteristic features of the traditional Japanese diet. Several studies indicate that these foods have beneficial effects on some cause-specific mortality(Reference Sauvaget, Nagano and Allen24, Reference Yamamoto, Sobue and Kobayashi25). In addition, although the use of near-median values as cut-points for the consumption of meat and tsukemono appears arbitrary, they were chosen in accordance with their use in the previous studies of the Mediterranean diet(Reference Trichopoulou, Costacou and Bamia2). Unfortunately, while the intake of tsukemono, infrequent consumption of soup with noodles and occasional drinking appeared to have the strongest association with a reduced risk of mortality, we cannot be certain that the other components of the Japanese diet are less important. As in any observational study, it is difficult to identify specific dietary effects due to multicollinearity that exists among food item intake. Within each component of the Japanese diet, there can also be considerable heterogeneity in nutrient content. In addition, overlap between components often occurs with the sharing of common ingredients or in how they are prepared and served. To better identify the effects of specific nutrients on mortality would require a controlled clinical trial. It may also be that dietary factors need to be considered in combination for an effect on longevity to be observed. An additional limitation is that we used mortality data as end points, which may lead to the misclassification of the cause of deaths. However, it has been reported that the death-certificate diagnosis for stroke and cancer in Japan is quite accurate(Reference Ron, Carter and Jablon26). Possible misclassification of acute myocardial infarction as ‘heart failure’ is also not an issue in the present report since both outcomes are collectively categorised as CVD(Reference Saito, Folsom and Aono27).

Conclusions

Adherence to a healthy Japanese diet was associated with an approximate 20 % lower rate of all-cause and cardiovascular mortality. While Japanese are exceptionally long lived, placing greater emphasis on the intake of foods that are low in salt could increase longevity in Japan further.

Acknowledgements

The authors thank all members of the Japanese Association of Public Health Center Directors and all staffs of the public health centres who cooperated with the present study. Investigators and members of the research group are listed in the appendix of Nakamura et al. (Reference Nakamura, Sato and Shimamoto11). Y. N. participated in designing and conducting the study, analysing and interpreting the data, and writing and preparing the manuscript. H. U. was the principal investigator and participated in designing and conducting the study and analysing and interpreting the data. T. O., R. D. A. and A. O. participated in conducting the study and analysing and interpreting the data. T. K. and Y. K. participated in managing and interpreting the data. T. H. participated in managing the data and conducting the study. We all have no conflicts of interest to disclose. The present study was supported by the Research Grant for Cardiovascular Diseases (7A-2) from the Ministry of Health, Labour and Welfare and a Health and Labour Sciences Research Grant, Japan (Comprehensive Research on Aging and Health: H11-Chouju-046, H14-Chouju-003, H17-Chouju-012), and by the Japan Society for the Promotion of Science Invitation Fellowship Programmes for Research, Japan.

Appendix NIPPON DATA80 Dietary Questionnaire

For each food listed on Q11–Q16, please check the box indicating how often you eat, on average

From Q22 to Q31, please choose one that fits best your recent eating habit

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Figure 0

Table 1 Baseline characteristics according to preferred food type – National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980, 1980–99*(Mean values and standard deviations)

Figure 1

Table 2 Baseline characteristics according to tertiles of the Reduced-Salt Japanese Diet Score among 4018 men and 5068 women – National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980, 1980–99*(Mean values and standard deviations)

Figure 2

Table 3 All-cause and cause-specific mortality according to Reduced-Salt Japanese Diet Score among 9089 men and women – National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980, 1980–99*(Hazard ratios (HR) and 95 % confidence intervals)

Figure 3

Table 4 Components of Reduced-Salt Japanese Diet Score and all-cause mortality among 9089 men and women – National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980, 1980–99*(Hazard ratios (HR) and 95 % confidence intervals)

Figure 4

Table 5 Variables used for propensity score matching and survival rate – National Integrated Project for Prospective Observation of Non-Communicable Diseases and its Trends in the Aged, 1980, 1980–99(Mean values and standard deviations)

Figure 5

Fig. 1 Kaplan–Meier survival curve after propensity score matching. Significant differences in the average propensity score and the variables used in its calculation before matching in the two groups disappeared after matching. By contrast, a significant difference between the matched survival curves remained as shown in the figure (P = 0·0003 by log-rank test). Survival differences by the group were significant when examined further using a regression model with a Weibull distribution that included adjustment for age and sex as the dependent variables (P < 0·0001). The thick line indicates survival for the participants with the Reduced-Salt Japanese Diet Score 4–7 and the thin line with the Reduced-Salt Japanese Diet Score 0–3.

Figure 6

Appendix NIPPON DATA80 Dietary Questionnaire

Figure 7

For each food listed on Q11–Q16, please check the box indicating how often you eat, on average

Figure 8

Figure 9

From Q22 to Q31, please choose one that fits best your recent eating habit