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Optimizing Electron Backscatter Diffraction of Carbonate Biominerals—Resin Type and Carbon Coating

Published online by Cambridge University Press:  22 May 2009

Alberto Pérez-Huerta  and
Maggie Cusack
Alberto Pérez-Huerta*
Affiliation:
Department of Geographical and Earth Sciences, University of Glasgow, G12 8QQ Glasgow, UK
Maggie Cusack
Affiliation:
Department of Geographical and Earth Sciences, University of Glasgow, G12 8QQ Glasgow, UK
*
Corresponding author. E-mail: Alberto.PerezHuerta@ges.gla.ac.uk
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Abstract

Electron backscatter diffraction (EBSD) is becoming a widely used technique to determine crystallographic orientation in biogenic carbonates. Despite this use, there is little information available on preparation for the analysis of biogenic carbonates. EBSD data are compared for biogenic aragonite and calcite in the common blue mussel, Mytilus edulis, using different types of resin and thicknesses of carbon coating. Results indicate that carbonate biomineral samples provide better EBSD results if they are embedded in resin, particularly epoxy resin. A uniform layer of carbon of 2.5 nm thickness provides sufficient conductivity for EBSD analyses of such insulators to avoid charging without masking the diffracted signal. Diffraction intensity decreases with carbon coating thickness of 5 nm or more. This study demonstrates the importance of optimizing sample preparation for EBSD analyses of insulators such as carbonate biominerals.

Keywords

aragonitecalcitemusselepoxydiffraction intensitynanometer
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 15 , Issue 3 , June 2009 , pp. 197 - 203
Copyright
Copyright © Microscopy Society of America 2009

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References

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