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The effect of feeding structured triacylglycerols enriched in eicosapentaenoic or docosahexaenoic acids on murine splenocyte fatty acid composition and leucocyte phagocytosis

Published online by Cambridge University Press:  09 March 2007

Samantha Kew
Affiliation:
Institute of Human Nutrition, University of Southampton, Southampton SO16 7PX, UK
Edward S. Gibbons
Affiliation:
Institute of Human Nutrition, University of Southampton, Southampton SO16 7PX, UK
Frank Thies
Affiliation:
Institute of Human Nutrition, University of Southampton, Southampton SO16 7PX, UK Department of Medicine and Therapeutics, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
Gerald P. McNeill
Affiliation:
Unilever Research Colworth Laboratory, Sharnbrook, Bedford MK44 1LQ, UK Loders Croklaan NA, Channahon, IL 60410, USA
Paul T. Quinlan
Affiliation:
Unilever Research Colworth Laboratory, Sharnbrook, Bedford MK44 1LQ, UK Unilever Health Institute, Unilever Research Vlaardingen, 3130 AC Vlaardingen, The Netherlands
Philip C. Calder*
Affiliation:
Institute of Human Nutrition, University of Southampton, Southampton SO16 7PX, UK
*
*Corresponding author: Professor P. C. Calder, fax +44 23 80595489, email pcc@soton.ac.uk
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Abstract

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The effects of altering the type of n-3 polyunsaturated fatty acid (PUFA) in the mouse diet on the ability of monocytes and neutrophils to perform phagocytosis were investigated. Male weanling mice were fed for 7 d on one of nine diets which contained 178 g lipid/kg and which differed in the type of n-3 PUFA and in the position of these in dietary triacylglycerol (TAG). The control diet contained 4·4 g α-linolenic acid/100 g total fatty acids. In the other diets, eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) replaced a proportion (50 or 100 %) of the α-linolenic acid, and were in the sn-2 or the sn-1(3) position of dietary TAG. There were significant increases in the content of n-3 PUFA in spleen-cell phospholipids when EPA or DHA was fed. These increases were largely independent of the position of EPA or DHA in dietary TAG except when EPA was fed at the highest level, when the incorporation was greater when it was fed in the sn-2 than in the sn-1(3) position. There was no significant effect of dietary DHA on monocyte or neutrophil phagocytic activity. Dietary EPA dose-dependently decreased the number of monocytes and neutrophils performing phagocytosis. However, when EPA was fed in the sn-2 position, the ability of active monocytes or neutrophils to engulf bacteria was increased in a dose-dependent fashion. This did not occur when EPA was fed in the sn-1(3) position. Thus, there appears to be an influence of the position of EPA, but not of DHA, in dietary TAG on its incorporation into cell phospholipids and on the activity of phagocytic cells.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2003

References

Babu, US, Bunning, VK, Wiesenfeld, P, Raybourne, RB & O'Donnell, M (1997) Effect of dietary flaxseed of fatty acid composition, superoxide, nitric oxide generation and antilisterial activity of peritoneal macrophages from female Sprague-Dawley rats. Life Sci 60, 545554.CrossRefGoogle ScholarPubMed
Bracco, U (1994) Effect of triacylglycerol structure on fat absorption. Am J Clin Nutr 60, 1002S1009S.10.1093/ajcn/60.6.1002SCrossRefGoogle ScholarPubMed
British Nutrition Foundation (1992) Report of the Task Force on Unsaturated Fatty Acids: Nutritional and Physiological Significance. London: Chapman and Hall.Google Scholar
British Nutrition Foundation (1999) Briefing Paper: n-3 Fatty Acids and Health. London: British Nutrition Foundation.Google Scholar
Calder, PC, Bond, JA, Harvey, DJ, Gordon, S & Newsholme, EA (1990) Uptake of saturated and unsaturated fatty acids into macrophage lipids and their effect upon macrophage adhesion and phagocytosis. Biochem J 269, 807814.10.1042/bj2690807CrossRefGoogle ScholarPubMed
D'Ambola, JB, Aeberhard, EE, Trang, N, Graffar, S, Barrett, CT & Sherman, MP (1991) Effect of dietary (n-3) and (n-6) fatty acids on in vivo pulmonary bacterial clearance by neonatal rabbits. J Nutr 121, 12621269.10.1093/jn/121.8.1262CrossRefGoogle ScholarPubMed
De Deckere, EAM, Korver, O, Vershuren, PM & Katan, MB (1998) Health aspects of fish and n-3 polyunsaturated fatty acids from plant and marine origin. Eur J Clin Nutr 152, 749753.CrossRefGoogle Scholar
Department of Health (1991) Dietary Reference Values for Food Energy and Nutrients for the United Kingdom. London: HM Stationery Office.Google Scholar
Eicher, SD & McVey, DS (1995) Dietary modulation of Kupffer cell and splenocyte function during Salmonella typhimurium challenge in mice. J Leuk Biol 58, 3239.10.1002/jlb.58.1.32CrossRefGoogle ScholarPubMed
Emken, EA, Adlof, RO, Duval, SM & Nelson, GJ (1999) Effect of dietary docosahexaenoic acid on desaturation and uptake in vivo of isotope-labeled oleic, linoleic, and linolenic acids by male subjects. Lipids 34, 785791.CrossRefGoogle ScholarPubMed
Garg, ML, Sebokova, E, Thomson, AB & Clandinin, MT (1988) Delta 6-desaturase activity in liver microsomes of rats fed diets enriched with cholesterol and/or omega 3 fatty acids. Biochem J 249, 351356.10.1042/bj2490351CrossRefGoogle ScholarPubMed
Gunstone, FD & Seth, S (1994) A study of the distribution of eicosapentaenoic acid and docosahexaenoic acid between the alpha and beta glycerol chains in fish oils by C-13-NMR spectroscopy. Chem Phys Lipids 72, 119126.CrossRefGoogle Scholar
Halvorsen, DA, Hansen, J-B, Grimsgaard, S, Bonna, KH, Kierulf, P & Nordoy, A (1997) The effect of highly purified eicosapentaenoic and docosahexaenoic acids on monocyte phagocytosis in man. Lipids 32, 935942.CrossRefGoogle ScholarPubMed
Hubbard, NE, Somers, SD & Erickson, KL (1991) Effect of dietary fishoil on development and selected functions of murine inflammatory macrophages.. J Leuk Biol 49, 592598.10.1002/jlb.49.6.592CrossRefGoogle Scholar
Hung, P, Gu, J-Y, Kaku, S, et al. . (2000) Dietary effects of eicosapentaenoic and docosahexaenoic acid esters on lipid metabolism and immune parameters in Sprague-Dawley rats. Biosci Biotech Biochem 64, 25882593.10.1271/bbb.64.2588CrossRefGoogle Scholar
Jeffery, NM, Newsholme, EA & Calder, PC (1997) The level of polyunsaturated fatty acids and the n -6 to n -3 polyunsaturated fatty acid ratio in the rat diet both affect serum lipid levels and lymphocyte functions. Prost Leuk Essent Fatty Acids 57, 149160.10.1016/S0952-3278(97)90005-3CrossRefGoogle Scholar
Lokesh, BR & Wrann, M (1984) Incorporation of palmitic acid or oleic acid into macrophage membrane lipids exerts differential effects on the function of normal mouse peritoneal macrophages. Biochim Biophys Acta 792, 141148.CrossRefGoogle ScholarPubMed
McNeill, GP, Ackman, RG & Moore, SR (1996) Lipase-catalysed enrichment of long-chain polyunsaturated fatty acids. J Am Oil Chem Soc 73, 14031407.CrossRefGoogle Scholar
Mahoney, EM, Hamill, AL, Scott, WA & Cohn, ZA (1977) Response of endocytosis to altered fatty acyl composition of macrophage phospholipids. Proc Natl Acad Sci USA 74, 48954899.10.1073/pnas.74.11.4895CrossRefGoogle ScholarPubMed
Moore, SR & McNeill, GP (1996) Production of triacylglycerols enriched in long-chain n-3 polyunsaturated fatty acids from fish oil. J Am Oil Chem Soc 73, 14091414.10.1007/BF02523504CrossRefGoogle Scholar
Peterson, LD, Jeffrey, NM, Thies, F, Sanderson, P, Newsholme, EA & Calder, PC (1998) Eicosapentaenoic and docosahexaenoic acids alter rat spleen leukocyte fatty acid composition and prostaglandin E 2 production but have different effects on lymphocyte functions and cell-mediated immunity. Lipids 33, 171180.10.1007/s11745-998-0193-yCrossRefGoogle ScholarPubMed
Schroit, AJ & Gallily, R (1979) Macrophage fatty acid composition and phagocytosis: effect of unsaturation on cellular phagocytic activity. Immunology 35, 199205.Google Scholar
Sipka, S, Dey, I, Buda, C, Csongor, J, Szegedi, G & Farkas, T (1996) The mechanism of inhibitory effect of eicosapentaenoic acid on phagocytic activity and chemotaxis of human neutrophil granulocytes. Clin Immunol Immunopathol 79, 224228.10.1006/clin.1996.0072CrossRefGoogle ScholarPubMed
Speert, DP (1992) Macrophages in bacterial infection. In The Macrophage pp. 215263 [Lewis, CE and McGee, JO'D, editors]. Oxford: IRL Press.Google Scholar
Stubbs, CD & Smith, AD (1984) The modification of mammalian membrane polyunsaturation and composition in relation to membrane fluidity and function. Biochim Biophys Acta 779, 89137.10.1016/0304-4157(84)90005-4CrossRefGoogle ScholarPubMed
Thies, F, Miles, EA, Nebe-von-Caron, G, et al. . (2001a) Influence of dietary supplementation with long-chain n-3 or n-6 polyunsaturated fatty acids on blood inflammatory cell populations and functions and on plasma soluble adhesion molecules in healthy humans. Lipids 36, 11831193.10.1007/s11745-001-0831-4CrossRefGoogle ScholarPubMed
Thies, F, Nebe-von-Caron, G, Powell, JR, Yaqoob, P, Newsholme, EA & Calder, PC (2001b) Dietary supplementation with gamma linolenic acid or fish oil decreases T lymphocyte proliferation in healthy older humans. J Nutr 131, 19181927.CrossRefGoogle ScholarPubMed
Thies, F, Nebe-von-Caron, G, Powell, JR, Yaqoob, P, Newsholme, EA & Calder, PC (2001c) Dietary supplementation with eicosapentaenoic acid, but not with other long chain n-3 or n-6 polyunsaturated fatty acids, decreases natural killer cell activity in healthy subjects aged >55 years. Am J Clin Nutr 73, 539548.CrossRefGoogle Scholar
Thies, F, Peterson, LD, Powell, JR, et al. . (1999) Manipulation of the type of fat consumed by growing pigs affects plasma and mononuclear cell fatty acid compositions and lymphocyte and phagocyte functions. J Anim Sci 77, 137147.10.2527/1999.771137xCrossRefGoogle ScholarPubMed
Turek, JJ, Schoenlein, IA, Clark, LK, Van Alstine, WG (1994) Dietary polyunsaturated fatty acids effects on immune cells of the porcine lung. J Leuk Biol 56, 599604.CrossRefGoogle ScholarPubMed
Virella, G, Kilpatrick, JM, Rugeles, MT, Hyman, B & Russell, R (1989) Depression of humoral responses and phagocytic functions in vivo and in vitro by fish oil and eicosapentaenoic acid. Clin Immunol Immunopathol 52, 257270.CrossRefGoogle Scholar
Yaqoob, P, Newsholme, EA & Calder, PC (1994a) The effect of dietary lipid manipulation on rat lymphocyte subsets and proliferation. Immunology 82, 603610.Google ScholarPubMed
Yaqoob, P, Newsholme, EA & Calder, PC (1994b) Inhibition of natural killer cell activity by dietary lipids. Immunol Lett 41, 241247.CrossRefGoogle ScholarPubMed