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Effect of melamine on in vitro rumen microbial growth, methane production and fermentation of Chinese wild rye hay and maize meal in binary mixtures

Published online by Cambridge University Press:  15 October 2013

H. J. YANG ,
H. ZHUANG ,
X. K. MENG ,
D. F. ZHANG  and
B. H. CAO
H. J. YANG*
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University (CAU), Beijing 100193, People's Republic of China
H. ZHUANG
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University (CAU), Beijing 100193, People's Republic of China
X. K. MENG
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University (CAU), Beijing 100193, People's Republic of China
D. F. ZHANG
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University (CAU), Beijing 100193, People's Republic of China
B. H. CAO
Affiliation:
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University (CAU), Beijing 100193, People's Republic of China
*
*To whom all correspondence should be addressed. Email: yang_hongjian@sina.com
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Summary

The effects of melamine on gas production (GP) kinetics, methane (CH4) production and fermentation of diets differing in forage content (low-forage (LF) diet: 200 g/kg and high-forage (HF) diet: 800 g/kg) by rumen micro-organisms in vitro were studied using batch cultures. Rumen contents were collected from three Simmental×Luxi crossbred beef cattle. Melamine was added to the incubation bottles to achieve final concentration of 0 (control), 2, 6, 18, 54, 162 and 484 mg/kg of each diet. Cumulative GP was continuously measured in an automated gas recording instrument during 72 h of incubation, while fermentation gas end-products were collected to determine molar proportions of carbon dioxide (CO2), CH4 and hydrogen gas (H2) in manually operated batch cultures. Differences in GP kinetics and fermentation gases were observed in response to the nature of the diets incubated. Although melamine addition did not affect GP kinetics and fermentation gas pattern compared to the control, the increase of melamine addition stimulated the yield of CH4 by decreasing CO2, especially during the fermentation of the HF diet. The concentrations of ammonia nitrogen (N), amino acid N and microbial N in culture fluids were greater in the fermentation of LF- than HF diets, and these concentrations were increased by the increase of melamine addition after 72-h fermentation. The concentrations of total volatile fatty acids (VFA) were greater in HF than LF diets. The addition of melamine decreased total VFA concentrations and this response was greater in HF than LF diet fermentations. Melamine addition did not affect molar proportions of acetate, butyrate, propionate and valerate compared with the control; however, branched-chain VFA production, which was lower in the HF than the LF diet, was increased by the melamine addition, especially in the HF diet fermentation. The ratio of non-glucogenic to glucogenic acids was lower in the HF than the LF diet, but it was not affected by melamine addition. In brief, the greater reduction in the rate and extent of rumen fermentation found for the HF diet in comparison with the LF diet suggested that rumen fermentation rate and extent in vitro depended mainly on the nature of the incubated substrate, and that they could be further inhibited by the increase of melamine addition.

Type
Animal Research Papers
Information
The Journal of Agricultural Science , Volume 152 , Issue 4 , August 2014 , pp. 686 - 696
Copyright
Copyright © Cambridge University Press 2013 

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References

REFERENCES

AOAC. (1999). Official Methods of Analysis, 16th edn, Washington, DC: Association of Analytical Chemists.Google Scholar
Bergman, E. N. (1973). Glucose metabolism in ruminants as related to hypoglycemia and ketosis. American Journal of Physiology 215, 865–873.CrossRefGoogle Scholar
Broderick, G. A. & Wallace, R. J. (1988). Effect of dietary nitrogen source on concentrations of ammonia, free amino acids and fluorescaminereactive peptides in the sheep rumen. Journal of Animal Science 66, 2233–2238.CrossRefGoogle Scholar
Broome, A. W. (1968). The use of non-protein nitrogen in animal feeds. In Proc., Univ. of Nottingham School of Agriculture Second Nutr. Conf. for Feed Manufacturers (Eds Swan, H. & Lewis, D.), pp. 92–113. London: J&A Churchill, Ltd.Google Scholar
Brown, C. A., Jeong, K. S., Poppenga, R. H., Puschner, B., Miller, D. M., Ellis, A. E., Kang, K. I., Sum, S., Cistola, A. M. & Brown, S. A. (2007). Outbreaks of renal failure associated with melamine and cyanuric acid in dogs and cats in 2004 and 2007. Journal of Veterinary Diagnostic Investigation 19, 525–531.CrossRefGoogle ScholarPubMed
Burroughs, W., Nelson, D. K. & Mertens, D. R. (1975). Protein physiology and its application in the lactating cow: the metabolizable protein feeding standard. Journal of Animal Science 41, 933–944.CrossRefGoogle ScholarPubMed
Bryant, M. P. (1973). Nutritional requirements of the predominant rumen cellulolytic bacteria. Federation Proceedings 32, 1809–1813.Google Scholar
Central People's Government of the People's Republic of China (CPGPRC) (2008). Proclamation 25, 2008 (in Chinese). Ministry of Health, Ministry of Industry and Information Technology, Ministry of Agriculture, State Administration For Industry & Commerce, General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China.Google Scholar
Central People's Government of the People's Republic of China (CPGPRC) (2009). Proclamation 1218 (in Chinese), June 8, 2009. Ministry of Agriculture of the People's Republic of China.Google Scholar
Chen, K. (2007). Anhui Province poisonous infant formula incident. In Public Health Security (in Chinese) (Ed. Chen, K.), pp. 169–170. Hangzhou City, China: Zhejiang University Press.Google Scholar
China Standard NY/T 815. (2004). Feeding Standard of Beef Cattle (in Chinese). China Nongye Hang Ye Biaozhun/Tuijian-815. Beijing, People's Republic of China: China Agriculture Publisher.Google Scholar
Clark, R., Barratt, E. L. & Kellerman, J. H. (1965). A comparison between nitrogen retention from urea, biuret, triuret and cyanuric acid by sheep on a low protein roughage diet. Journal of the South African Veterinary Association 36, 79–80.Google Scholar
Cotta, M. A. & Russell, J. B. (1982). Effect of peptides and amino acids onefficiency of rumen bacterial protein synthesis in continuous culture. Journal of Dairy Science 65, 226–234.Google Scholar
Demeyer, D. I. & Henderickx, H. K. (1967) Methane production from glucose in vitro by mixed rumen bacteria. The Biochemical Journal 105, 271–277.CrossRefGoogle ScholarPubMed
France, J., Dijkstra, J., Dhanoa, M. S., López, S. & Bannink, A. (2000). Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: derivation of models and other mathematical considerations. British Journal of Nutrition 83, 143–150.CrossRefGoogle ScholarPubMed
García-Martínez, R., Ranilla, M. J., Tejido, M. L. & Carro, M. D. (2005). Effects of disodium fumarate on in vitro rumen microbial growth, methane production and fermentation of diets differing in their forage:concentrate ratio. British Journal of Nutrition 94, 71–77.CrossRefGoogle ScholarPubMed
Griswold, K. E., Hoover, W. H., Miller, T. K. & Thayne, W. V. (1996). Effect of form of nitrogen on growth of ruminal microbes in continuous culture. Journal of Animal Science 74, 483–491.Google Scholar
Griswold, K. E., Apgar, G. A., Bouton, J. & Firkins, J. L. (2003). Effects of urea infusion and ruminal degradable protein concentration on microbial growth, digestibility and fermentation in continuous culture. Journal of Animal Science 81, 329–336.CrossRefGoogle ScholarPubMed
Hristov, A. N., Etter, R. P., Ropp, J. K. & Grandeen, K. L. (2004). Effect of dietary crude protein level and degradability on ruminal fermentation and nitrogen utilization in lactating dairy cows. Journal of Animal Science 82, 3219–3229.Google Scholar
Hungate, R. E. (1966). The Rumen and its Microbes. New York: Academic Press, Inc.Google Scholar
Hungate, R. E., Smith, W., Bauchop, T., Yu, I. & Rabinowitz, J. C. (1970). Formate as an intermediate in the bovine rumen fermentation. Journal of Bacteriology 102, 389–397.CrossRefGoogle ScholarPubMed
Ingelfinger, J. R. (2008). Melamine and the global implications of food contamination. New England Journal of Medicine 359, 2745–2747.Google Scholar
Jones, D. F., Hoover, W. H. & Miller Webster, T. K. (1998). Effects of concentrations of peptides on microbial metabolism in continuous culture. Journal of Animal Science 76, 611–616.Google Scholar
Krishnamoorthy, U., Soller, H., Steingass, H. & Menke, K. H. (1991). A comparative study on rumen fermentation of energy supplements in vitro. Journal of Animal Physiology and Animal Nutrition 65, 28–35.CrossRefGoogle Scholar
Makkar, H. P. S. & Becker, K. (1999). Purine quantification in digesta from ruminants by spectrophotometric and HPLC methods. British Journal of Nutrition 81, 107–112.CrossRefGoogle ScholarPubMed
Menke, K. H. & Steingass, H. (1988). Estimation of the energetic feed value obtained by chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28, 7–55.Google Scholar
Menke, K. H., Raab, L., Salewski, A., Steingass, H., Fritz, D. & Schneider, W. (1979). The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science, Cambridge 93, 217–222.Google Scholar
Merry, R. J., Theodorou, M. K., Raurich, M. G. & Dhanoa, M. S. (1991). Use of head space gas pressure in batch cultures to assist in determination of nutritive value of silage to rumen bacteria. In Conference on Forage Conservation Towards 2000, Braunschweig, Germany, 23–25 January 1991 (Eds Pahlow, G. & Honig, H.), pp. 451–454. Landbauforschung Völkenrode 12. Braunschweig, Germany: European Grassland Federation.Google Scholar
Nagaraja, T. G., Newbold, C. J., Van Nevel, C. J. & Demeyer, D. I. (1997). Manipulation of ruminal fermentation. In The Rumen Microbial Ecosystem (Eds Hobson, P. N. & Stewart, C. S.), pp. 523–632. London: Chapman & Hall.Google Scholar
Newton, G. L. & Utley, P. R. (1978). Melamine as a dietary nitrogen source for ruminants. Journal of Animal Science 47, 1338–1344.Google Scholar
Ørskov, E. R. (1975). Manipulation of rumen fermentation for maximium food utilization. In World Review of Nutrition and Dietetics, Vol. 22 (Ed. Bourne, G. H.), pp. 152–182. Basel, Switzerland: Karger Medical and Scientific Publishers.Google Scholar
Reynal, S. M. & Broderick, G. A. (2005). Effect of dietary level of rumen degraded protein on production and nitrogen metabolism in lactating dairy cows. Journal of Dairy Science 88, 4045–4064.Google Scholar
Reynal, S. M., Ipharraguerre, I. R., Liñeiro, M., Brito, A. F., Broderick, G. A. & Clark, J. H. (2007). Omasal flow of soluble proteins, peptides and free amino acids in dairy cows fed diets supplemented with proteins of varying ruminal degradabilities. Journal of Dairy Science 90, 1887–1903.Google Scholar
Rohlf, M. E. (1974). Improvement in NPN utilization by ‘slow release’ or ‘sustained release’. In Proceedings of the 34th Annual Meeting, American Feed Manufacturers Association. p. 11–13. Arlington, VA: AMFA.Google Scholar
Russell, J. B. & Sniffen, C. J. (1984). Effect of carbon-4 and carbon-5 volatile fatty acids on growth of mixed rumen bacteria in vitro. Journal of Dairy Science 67, 987–994.Google Scholar
SAS (1999). Statistical Analytical System (SAS) Users Guide: Statistics, Version 8.2. Cary, NC: Statistical Analysis Institute.Google Scholar
Satter, L. D. & Roffler, R. E. (1975). Nitrogen requirement and utilization in dairy cattle. Journal of Dairy Science 58, 1219–1224.Google Scholar
Satter, L. D. & Slyter, L. L. (1974). Effect of ammonia concentration on rumen microbial protein production in vitro. British Journal of Nutrition 32, 199–208.Google Scholar
Shen, J. S., Wang, J. Q., Wei, H. Y., Bu, D. P., Sun, P. & Zhou, L. Y. (2010). Transfer efficiency of melamine from feed to milk in lactating dairy cows fed with different doses of melamine. Journal of Dairy Science 93, 2060–2066.CrossRefGoogle ScholarPubMed
Song, M. K. & Kennelly, J. J. (1989). Effect of ammoniated barley silage on ruminal fermentation, nitrogen supply to the small intestine, ruminal and whole tract digestion and milk production of Holstein cows. Journal of Dairy Science 72, 2981–2990.CrossRefGoogle Scholar
Turnipseed, S., Casey, C., Nochetto, C. & Heller, D. N. (2008). Determination of Melamine and Cyanuric Acid Residues. Laboratory Information Bulletin no. 4421, Vol. 24. College Park, MD: Center for Food Safety & Applied Nutrition, October 2008.Google Scholar
USFDA (2008). Melamine Contamination in China. Silver Spring, MD: USFDA. Available from: http://www.fda.gov/NewsEvents/PublicHealthFocus/ucm179005.htm (verified 23 July 2013).Google Scholar
Van der Meer, J. M., Wever, G. & Bediye, S. (1988). Rumen bacteria for evaluation of enzymatically changed animal feeds and genetic varieties of fodder plants. Analytica Chimica Acta 213, 177–185.Google Scholar
Van Soest, P. J., Robertson, J. B. & Lewis, B. A. (1991). Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.Google Scholar
Verdouw, H., Van Echteld, C. J. A. & Dekkers, E. M. J. (1978). Ammonia determination based on indophenol formation with sodium salicylate. Water Research 12, 399–402.Google Scholar
Wallace, R. J. (1979). Effect of ammonia concentration on the composition, hydrolytic activity and nitrogen metabolism of the microbial flora of the rumen. Journal of Applied Bacteriology 47, 443–449.Google Scholar
Yang, C. M. J. (2002). Response of forage fiber degradation by ruminal microorganisms to branched-chain volatile fatty acids, amino acids and dipeptides. Journal of Dairy Science 85, 1183–1190.Google Scholar
Zhang, D. F. & Yang, H. J. (2011). In vitro ruminal methanogenesis of a hay-rich substrate in response to different combination supplements of nitrocompounds, pyromellitic diimideand, 2-bromoethanesulphonate. Animal Feed Science and Technology 163, 20–32.CrossRefGoogle Scholar
Zinn, R. A. & Owen, F. N. (1986). A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis. Canadian Journal of Animal Science 66, 157–166.Google Scholar