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Production of Highly Ordered Nanoporous Alumina and its Application in Cell Cultivation

Published online by Cambridge University Press:  01 February 2011

Andreas Hoess
Affiliation:
andreas.hoess@iwmh.fraunhofer.de, Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany, +49345 5589286, +49345 5589101
Andrea Staeudte
Affiliation:
andrea.staeudte@iwmh.fraunhofer.de, Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany
Annika Thormann
Affiliation:
annika.thormann@iwmh.fraunhofer.de, Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany
Martin Steinhart
Affiliation:
steinhar@mpi-halle.mpg.de, Max Planck Institute of Microstructure Physics, Experimental Department II, Weinberg 2, Halle, 06120, Germany
Andreas Heilmann
Affiliation:
andreas.heilmann@iwmh.fraunhofer.de, Fraunhofer Institute for Mechanics of Materials, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle, 06120, Germany
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Abstract

Nanoporous alumina membranes were utilized as cell culture substrates. Two different anodization processes were applied in order to obtain highly ordered, self-supporting nanoporous alumina membranes. The resulting membranes exhibit uniform nanopores with constant diameters having aspect ratios (pore depth:pore diameter) larger than 1000. Tentative experiments evaluating the cellular interaction of a hepatoma cell line (HepG2) with the nanoporous alumina membranes will be described. Furthermore, cell proliferation as well as the adhesion behavior of the cells on the porous substrates was investigated. Scanning electron microscopy (SEM) and focused ion beam (FIB) technology were applied to examine the cell morphology. The experiments revealed that the nanoporous membranes can be used as cell culture substrates and that they have no adverse effects on cell proliferation and cell viability. Besides, cells on membranes with pore diameters larger than 200 nm developed small cell extensions (filopodia) which penetrated into the nanopores.

Type
Research Article
Copyright
Copyright © Materials Research Society 2008

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References

1. Furneaux, R.C., Rigby, W.R. and Davidson, A.P., Nature 337, 147 (1989)Google Scholar
2. Thompson, G.E., Thin Solid Films 297, 192 (1997)Google Scholar
3. Jessensky, O., Müller, F. and Gösele, U., J. Electrochem. Soc. 145, 3735 (1998)Google Scholar
4. Popat, K.C., Swan, E.E.L., Mukhatyar, V., Chatvanichkul, K.-I., Mor, G.K., Grimes, C.A. and Desai, T.A., Biomaterials 26, 4516 (2005)Google Scholar
5. Swan, E.E.L., Popat, K.C., Grimes, C.A. and Desai, T.A., J. Biomed. Mater. Res. A 72, 288 (2005)Google Scholar
6. Briggs, E.P., Walpole, A.R., Wilshaw, P.R., Karlsson, M. and Pålsgård, E., J. Mater. Sci.-Mater. Med. 15, 1021 (2004)Google Scholar
7. Karlsson, M., Johansson, A., Tang, L. and Boman, M., Microsc. Res. Tech. 63, 259 (2004)Google Scholar
8. Thormann, A., Teuscher, N., Pfannmöller, M., Rothe, U. and Heilmann, A., Small 3, 1032 (2007)Google Scholar
9. Masuda, H. and Fukuda, K., Science 268, 1466 (1995)Google Scholar
10. Masuda, H., Yada, K. and Osaka, A., Jpn. J. Appl. Phys. 37, L1340 (1998)Google Scholar