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Using Peptide Hetero-assembly to Trigger Physical Gelation and Cell Encapsulation

Published online by Cambridge University Press:  01 February 2011

Andreina Parisi-Amon
Affiliation:
andreina@stanford.edu, Stanford University, Bioengineering, Stanford, California, United States
Cheryl Wong Po Foo
Affiliation:
cherylwongpofoo@gmail.com, Stanford University, Materials Science and Engineering, Stanford, California, United States
Ji Seok Lee
Affiliation:
jiseok.lee@stanford.edu, Stanford University, Materials Science and Engineering, Stanford, California, United States
Widya Mulyasasmita
Affiliation:
widyam@stanford.edu, Stanford University, Bioengineering, Stanford, California, United States
Sarah Heilshorn
Affiliation:
heilshorn@stanford.edu, Stanford University, Materials Science and Engineering, 476 Lomita Mall, McCullough Building, Room 246, Stanford, California, 94305-4045, United States
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Abstract

Stem cell transplantation holds tremendous potential for the treatment of various trauma and diseases. However, the therapeutic efficacy is often limited by poor and unpredictable post-transplantation cell survival. While hydrogels are thought to be ideal scaffolds, the sol-gel phase transitions required for cell encapsulation within commercially available biomatrices such as collagen and Matrigel often rely on non-physiological environmental triggers (e.g., pH and temperature shifts), which are detrimental to cells. To address this limitation, we have designed a novel class of protein biomaterials: Mixing-Induced Two-Component Hydrogels (MITCH) that are recombinantly engineered to undergo gelation by hetero-assembly upon mixing at constant physiological conditions, thereby enabling simple, biocompatible cell encapsulation and transplantation protocols. Building upon bio-mimicry and precise molecular-level design principles, the resulting hydrogels have tunable viscoelasticity consistent with simple polymer physics considerations. MITCH are reproducible across cell-culture systems, supporting growth of human endothelial cells, rat mesenchymal stem cells, rat neural stem cells, and human adipose-derived stem cells. Additionally, MITCH promote the differentiation of neural progenitors into neuronal phenotypes, which adopt a 3D-branched morphology within the hydrogels.

Type
Research Article
Copyright
Copyright © Materials Research Society 2010

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References

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