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Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis

Published online by Cambridge University Press:  25 March 2009

E. Hernández-Cruz
Affiliation:
Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México
J.J. González-Cabriales
Affiliation:
Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México
C. Ordaz-Pichardo
Affiliation:
Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México
N.I. de la Cruz-Hernández
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia ‘Dr. Norberto Treviño Zapata’, Universidad Autónoma de Tamaulipas, Victoria, Tamaulipas, México
G.H. Flores-Gutiérrez*
Affiliation:
Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa, Tamaulipas, México
*
*Fax: +52 899 923 0622 E-mail: ghflores@uat.edu.mx

Abstract

An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08 mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P < 0.05, kappa = 0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2009

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References

Agudelo, P., Botero, D. & Palacio, L.G. (2005) Evaluation of the ELISA method for diagnosis of human cysticercosis in an endemic region. Biomedica 25, 488495.CrossRefGoogle Scholar
Arambulo, P.V., Walls, K.W., Bullock, S. & Kagan, I.G. (1978) Serodiagnosis of human cysticercosis by microplate enzyme-linked immunospecific assay (ELISA). Acta Tropica 35, 6367.Google ScholarPubMed
Baveja, U.K., Arya, G., Goyle, V., Sharma, D.K. & Gupta, M. (1990) Enzyme linked immunosorbent assay for the diagnosis of human neurocysticercosis: a preliminary report. Journal of Communicable Diseases 22, 5559.Google ScholarPubMed
Biswas, R., Parija, S.C. & Narayan, S.K. (2004) Dot-ELISA for the diagnosis of neurocysticercosis. Revista do Instituto de Medicina Tropical de São Paulo 46, 249252.CrossRefGoogle ScholarPubMed
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248254.CrossRefGoogle ScholarPubMed
Bruckner, D.A. (1985) Serologic and intradermal tests for parasitic infections. Pediatric Clinics of North America 32, 10631075.CrossRefGoogle ScholarPubMed
Bucardo, F., Meza-Lucas, A., Espinoza, F., Garcia-Jeronimo, R.C., Garcia-Rodea, R. & Correa, D. (2005) The seroprevalence of Taenia solium cysticercosis among epileptic patients in Leon, Nicaragua, as evaluated by ELISA and western blotting. Annals of Tropical Medicine and Parasitology 99, 4145.CrossRefGoogle ScholarPubMed
Correa, D., Sandoval, M.A., Harrison, L.J., Parkhouse, R.M., Plancarte, A., Meza-Lucas, A. & Flisser, A. (1989) Human neurocysticercosis: comparison of enzyme immunoassay capture techniques based on monoclonal and polyclonal antibodies for the detection of parasite products in cerebrospinal fluid. Transactions of the Royal Society of Tropical Medicine and Hygiene 83, 814816.CrossRefGoogle ScholarPubMed
Costa, J.M. (1986) Immunoenzymatic test (ELISA) in the diagnosis of neurocysticercosis: study of various antigenic extracts in the detection of IgG antibodies in serum and cerebrospinal fluid samples. Arquivos de Neuro-psiquiatria 44, 1531.CrossRefGoogle Scholar
Ferreira, A.P., Vaz, A.J., Nakamura, P.M., Sasaki, A.T., Ferreira, A.W. & Livramento, J.A. (1997) Haemagglutination test for the diagnosis of human neurocystycercosis: development of a stable reagent using homologous and heterologous antigens. Revista do Instituto de Medicina Tropical de São Paulo 39, 2933.CrossRefGoogle ScholarPubMed
Fleury, A., Bouteille, B., Garcia, E., Marquez, C., Preux, P.M., Escobedo, F., Sotelo, J. & Dumas, M. (2001) Neurocysticercosis: validity of ELISA after storage of whole blood and cerebrospinal fluid on paper. Tropical Medicine and International Health 6, 688693.CrossRefGoogle ScholarPubMed
Flores-Gutiérrez, G.H., Infante, F., Salinas-Meléndez, J.A., Thomas, C.B., Estrada-Bellmann, P.C. & Briones-Encinia, F. (2004) Development of an immunobinding assay with monoclonal antibodies to diagnose Mycoplasma bovis in semen. Veterinary Research Communications 28, 681686.CrossRefGoogle ScholarPubMed
Gonzalez, A.E., Cama, V., Gilman, R.H., Tsang, V.C., Pilcher, J.B., Chavera, A., Castro, M., Montenegro, T., Verastegui, M., Miranda, E. & Bazalar, H. (1990) Prevalence and comparison of serologic assays, necropsy, and tongue examination for the diagnosis of porcine cysticercosis in Peru. American Journal of Tropical Medicine and Hygiene 43, 194199.CrossRefGoogle ScholarPubMed
Husain, N., Jyotsna, , Bagchi, M., Husain, M., Mishra, M.K. & Gupta, S. (2001) Evaluation of Cysticercus fasciolaris antigen for the immunodiagnosis of neurocysticercosis. Neurology India 49, 375379.Google ScholarPubMed
Infante, M.F., Reyna, M.O. & Farias, H.P. (1997) Establecimiento y evaluación del sistema avidin-biotina sobre papel de nitrocelulosa, para el serodiagnóstico de cisticercosis porcina. Veterinaria Mexico 28, 287290.Google Scholar
Infante, F., Infante, F. Jr & Flores-Gutiérrez, G.H. (2002) Improved immunobinding test using monoclonal antibodies for detection of Mycoplasma bovis in milk. Canadian Journal of Veterinary Research 66, 282284.Google ScholarPubMed
Kaur, M., Joshi, K., Ganguly, N.K., Mahajan, R.C. & Malla, N. (1995) Evaluation of the efficacy of albendazole against the larvae of Taenia solium in experimentally infected pigs, and kinetics of the immune response. International Journal for Parasitology 25, 14431450.CrossRefGoogle ScholarPubMed
Morakote, N., Nawacharoen, W., Sukonthasun, K., Thammasonthi, W. & Khamboonruang, C. (1992) Comparison of cysticercus extract, cyst fluid and Taenia saginata extract for use in ELISA for serodiagnosis of neurocysticercosis. The Southeast Asian Journal of Tropical Medicine and Public Health 23, 7781.Google ScholarPubMed
Nsengiyumva, G., Druet-Cabanac, M., Ramanankandrasana, B., Bouteille, B., Nsizabira, L. & Preux, P.M. (2003) Cysticercosis as a major risk factor for epilepsy in Burundi, east Africa. Epilepsia 44, 950955.CrossRefGoogle Scholar
Odashima, N.S., Takayanagui, O.M. & Figueiredo, J.F. (2002) Enzyme linked immunosorbent assay (ELISA) for the detection of IgG, IgM, IgE and IgA against Cysticercus cellulosae in cerebrospinal fluid of patients with neurocysticercosis. Arquivos de Neuro-psiquiatria 60, 400405.CrossRefGoogle ScholarPubMed
Pammenter, M.D. & Rossouw, E.J. (1987) The value of an antigenic fraction of Cysticercus cellulosae in the serodiagnosis of cysticercosis. Annals of Tropical Medicine and Parasitology 81, 117123.CrossRefGoogle ScholarPubMed
Peralta, R.H., Vaz, A.J., Pardini, A., Macedo, H.W., Machado, L.R., De Simone, S.G. & Peralta, J.M. (2002) Evaluation of an antigen from Taenia crassiceps cysticercus for the serodiagnosis of neurocysticercosis. Acta Tropica 83, 159168.CrossRefGoogle ScholarPubMed
Rodriguez-Canul, R., Fraser, A., Allan, J.C., Dominguez-Alpizar, J.L., Argaez-Rodriguez, F. & Craig, P.S. (1999) Epidemiological study of Taenia solium taeniasis/cysticercosis in a rural village in Yucatan state, Mexico. Annals of Tropical Medicine and Parasitology 93, 5767.Google Scholar
Rosas, N., Sotelo, J. & Nieto, D. (1986) ELISA in the diagnosis of neurocysticercosis. Archives of Neurology 43, 353356.CrossRefGoogle ScholarPubMed
Santellano-Estrada, E., Infante, F., Díaz-Aparicio, E. & Flores-Gutiérrez, G.H. (2004) Use of an immunobinding test on nitrocellulose paper to diagnose caprine brucellosis. Veterinary Research Communications 28, 2731.CrossRefGoogle ScholarPubMed
Vaz, A.J., Ferreira, A.W., Camargo, M.E., Nakamura, P.M. & Camargo, E.D. (1990) DOT-ELISA for detection of anti-Cysticercus cellulosae antibodies in human cerebrospinal fluid using a new solid-phase (resin-treated polyester fabrics). Preliminary report. Revista do Instituto de Medicina Tropical de São Paulo 32, 355359.CrossRefGoogle ScholarPubMed
Vaz, A.J., Nakamura, P.M., Camargo, M.E., Camargo, E.D. & Ferreira, A.W. (1996) Dot-ELISA for the detection of anti-Cysticercus cellulosae antibodies in cerebrospinal fluid using a new solid phase (resin-treated polyester fabric) and Cysticercus longicollis antigens. Revista do Instituto de Medicina Tropical de São Paulo 38, 391396.CrossRefGoogle ScholarPubMed
Vega, R., Pinero, D., Ramanankandrasana, B., Dumas, M., Bouteille, B., Fleury, A., Sciutto, E., Larralde, C. & Fragoso, G. (2003) Population genetic structure of Taenia solium from Madagascar and Mexico: implications for clinical profile diversity and immunological technology. International Journal for Parasitology 33, 14791485.CrossRefGoogle ScholarPubMed
Vianna, L.G., Costa-Cruz, J.M., Macedo, V., De Souza, D. & Moreira, D.G. (1992) Comparative study of immunoenzyme tests ELISA-G and ELISA-M, indirect immunofluorescence, and complement fixation in the diagnosis of human cysticercosis. Arquivos de Neuro-psiquiatria 50, 302308.CrossRefGoogle ScholarPubMed