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Immunoprecipitation of malarial acid endopeptidase

Published online by Cambridge University Press:  06 April 2009

E. Hempelmann ,
B. Putfarken ,
K. Rangachari  and
R. J. M. Wilson
E. Hempelmann
Affiliation:
Institut für Biochemie II, Im Neuenheimer Feld 328, D-6900 Heidelberg, FRG
B. Putfarken
Affiliation:
Division of Biochemistry, Bernhard-Nocht-Institut, Bernhard-Nocht-Strasse 74, D-2000 Hamburg 4, FRG
K. Rangachari
Affiliation:
Medical Research Council, Cell Biophysics Unit, King's College, Drury Lane, London WC2
R. J. M. Wilson
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

Electrophoresis of extracts of schizonts of Plasmodium knowlesi in non-dissociating polyacrylamide gels, separates several bands of acid endopeptidase activity. A polyclonal antiserum, produced by immunization with purified merozoites, failed to distinguish between different bands of the parasite enzyme, indicating that they are serologically related. Apart from the loss of one minor peak, extraction in Triton X-100 did not reduce the enzyme's electrophoretic heterogeneity. The antiserum did not react with red cell acid proteases.

Type
Research Article
Information
Parasitology , Volume 92 , Issue 2 , April 1986 , pp. 305 - 312
Copyright
Copyright © Cambridge University Press 1986

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References

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