Hostname: page-component-8448b6f56d-gtxcr Total loading time: 0 Render date: 2024-04-24T03:23:29.677Z Has data issue: false hasContentIssue false
  • English
  • Français

The dominance of exrB over exrB+ in heterodiploids of Escherichia coli*

Published online by Cambridge University Press:  14 April 2009

Joseph Greenberg ,
John Donch  and
Leonard Berends
Joseph Greenberg
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
John Donch
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
Leonard Berends
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

To test whether exrB, a mutation in Escherichia coli B making this strain sensitive to ultraviolet radiation and unable to divide normally, is dominant to exrB+, merodiploids were constructed by crossing Hfr exrB metA+his × F−malB met A thr proA recA. metA+recombinants were fertile (F′) and sensitive to UV. One of these, F′ exrB proA, was crossed with an F−metA malB thr, selecting for metA+proA+. All such progeny acquiring malB+ were UV-sensitive, fertile and segregated met mal UV-resistant progeny. They were, therefore, meriodiploids, F′ exrB/exrB+. exrB is dominant to exrB+, in which respect it resembles exrA (lex).

Type
Research Article
Information
Genetics Research , Volume 25 , Issue 1 , February 1975 , pp. 39 - 44
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Donch, J., Green, M. H. L. & Greenberg, J. (1968). Interaction of the exr and lon genes in Escherichia coli. Journal of Bacteriology 96, 1704–1710.CrossRefGoogle ScholarPubMed
Donch, J. & Greenberg, J. (1968 a). Loci of radiation sensitivity in Bs strains of Escherichia coli. Genetical Research 11, 183–191.CrossRefGoogle Scholar
Donch, J. & Greenberg, J. (1968 b). Genetic analysis of lon mutants of strain K-12 of Escherichia coli. Molecular general Genetics 103, 105–115.CrossRefGoogle ScholarPubMed
Donch, J. & Greenberg, J. (1974). The effect of lex on UV sensitivity, filament formation and λ induction in lon mutants of Escherichia coli. Molecular general Genetics 128, 277–281.CrossRefGoogle Scholar
Fangman, W. L. & Novick, A. (1968). Characterization of two bacterial mutants with temperature-sensitive synthesis of DNA. Genetics 60, 1–17.CrossRefGoogle ScholarPubMed
Greenberg, J., Berends, L. J., Donch, J. & Green, M. H. L. (1974). exrB. A malB-linked gene in Escherichia coli B involved in sensitivity to radiation and filament formation. Genetical Research 23, 175–184.CrossRefGoogle ScholarPubMed
Ingels, N. B., Daughters, G. T. & Burzio, A. (1968). New design for an automated bacterial colony counter. Review of Scientific Instruments 39, 115–119.CrossRefGoogle Scholar
Low, B. (1968). Formation of merodiploids in matings with a class of rec− recipient strains of Escherichia coli K12. Proceedings of the National Academy of Sciences, Washington 60, 160–167.CrossRefGoogle ScholarPubMed
Mount, D. W., Low, K. B. & Edmiston, S. J. (1972). Dominant mutations (lex) in Escherichia coli K12 which affect radiation sensitivity and frequency of ultraviolet light-induced mutations. Journal of Bacteriology 112, 886–893.Google Scholar
Mount, D. W., Walker, A. C. & Kosel, C. (1973). Suppression of lex mutations affecting deoxyribonucleic acid repair in E. coli K12 by closely linked thermosensitive mutations. Journal of Bacteriology 116, 950–956.CrossRefGoogle Scholar
Wechsler, J. A. & Gross, J. D. (1971). Escherichia coli mutants temperature-sensitive for DNA synthesis. Molecular general Genetics 113, 273–284.CrossRefGoogle ScholarPubMed