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Diversity of seed storage proteins in common wheat (Triticum aestivum L.)

Published online by Cambridge University Press:  25 May 2011

Zuzana Šramková
Affiliation:
Institute of Biochemistry, Nutrition and Health Protection, Slovak University of Technology, Bratislava, Slovakia
Edita Gregová*
Affiliation:
Plant Production Research Center, Plant Production Research Institute, Pieštany, Slovakia
Svetlana Šliková
Affiliation:
Plant Production Research Center, Plant Production Research Institute, Pieštany, Slovakia
Ernest Šturdík
Affiliation:
Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovakia
*
*Corresponding author. E-mail: gregova@vurv.sk

Abstract

The objective of our study was to determine the composition of high-molecular weight-glutenin subunits (HMW-GS) in 120 cultivars of common wheat (Triticum aestivum L.). Fourteen alleles and 34 allelic compositions were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1 and Glu-D1 loci were null (57.1%), 7+9 (43.3%) and 5+10 (61.9%), respectively. However, low-frequency HMW-GS alleles were also observed, such as 13+16, 20, 21, 7 and 18, encoded by the Glu-B1 locus, and 4+12, encoded by the Glu-D1 locus. The wheat–rye 1BL.1RS translocation was identified in 25 cultivars, using acid polyacrylamide gel electrophoresis. The Glu-score varied greatly, and some lines reached the maximum value of 10.

Type
Research Article
Copyright
Copyright © NIAB 2011

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