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Development and application of EST-SSRs for diversity analysis in Ethiopian grass pea

Published online by Cambridge University Press:  16 March 2011

M. Ponnaiah ,
E. Shiferaw ,
M. E. Pè  and
E. Porceddu
M. Ponnaiah*
Affiliation:
Scuola Superiore Sant'Anna, Piazza Martiri della Libertà, 33-56127Pisa, Italy
E. Shiferaw
Affiliation:
Scuola Superiore Sant'Anna, Piazza Martiri della Libertà, 33-56127Pisa, Italy
M. E. Pè
Affiliation:
Scuola Superiore Sant'Anna, Piazza Martiri della Libertà, 33-56127Pisa, Italy
E. Porceddu
Affiliation:
DABAC, University of Tuscia, Via S. C. De Lellis, 01100Viterbo, Italy
*
*Corresponding author. E-mail: m.ponnaiah@sssup.it
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Abstract

To date, very limited molecular knowledge and molecular tools are available on grass pea (Lathyrus sativus L.). Herein, we present a genetic diversity analysis of 20 grass pea accessions collected from various regions of Ethiopia by means of seven newly developed Lathyrus expressed sequence tag-derived simple sequence repeat (EST-SSR) markers and four cross-transferable EST-SSRs derived from Medicago truncatula L. Forty-five alleles were detected among all the analyzed accessions. The number of alleles/locus ranged from two to seven with an average of four alleles/locus. The observed heterozygosity (Ho) ranged from 0.320 to 0.504, while the expected heterozygosity (He) ranged from 0.354 to 0.470. FST values estimated by analysis of molecular variance were 0.01, 0.15 and 0.84 for among regions, among accessions and within accessions, respectively, indicating that most of the variation (84%) resides within accession. The model-based cluster analysis grouped the accessions into three clusters grouping accessions, irrespective of their collection regions.

Keywords

expressed sequence tag-derived simple sequence repeatgenetic diversityLathyrus
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 276 - 280
Copyright
Copyright © NIAB 2011

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References

CSA(2007) Agricultural sample survey 2006/2007. Report on area and production of crops volume I. Ethiopia: Central Statistics Authority.Google Scholar
Campbell, CG (1997) Grass pea. Lathyrus sativus L. Promoting the conservation and use of underutilized and neglected crops. 18. Rome: Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute.Google Scholar
Chowdhury, MA and Slinkard, AE (1997) Natural outcrossing in grasspea. J Hered 88: 154–156.CrossRefGoogle Scholar
Chowdhury, MA and Slinkard, AE (2000) Genetic diversity in grass pea (Lathyrus sativus L.). Genetic Resources and Crop Evolution 47: 163–169.Google Scholar
Ellis, JR and Burke, JM (2007) EST-SSRs as a resource for population genetic analyses. Heredity 99: 125–132.Google Scholar
Eujayl, I, Sledge, MK, Wang, L, May, GD, Chekhovskiy, K, Zwonitzer, JC and Mian, MAR (2004) Medicago truncatula EST-SSRs reveal cross-species genetic markers for Medicago spp. Theoretical and Applied Genetics 108: 414–422.Google Scholar
Falush, D, Stephens, M and Pritchard, JK (2003) Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164: 1567–1587.Google Scholar
Gupta, PK, Rustgi, S, Sharma, S, Singh, R, Kumar, N and Balyan, HS (2003) Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat. Molecular Genetics and Genomics 270: 315–323.Google Scholar
Gutiérrez-Marcos, JF, Vaquero, F, Sáenz de Miera, LE and Vences, FJ (2006) High genetic diversity in a world-wide collection of Lathyrus sativus L. revealed by isozymatic analysis. Plant Genetic Resources 4: 159–171.Google Scholar
Gutierrez, MV, Vaz Patto, MC, Huguet, T, Cubero, JI, Moreno, MT and Torres, AM (2005) Cross-species amplification of Medicago truncatula microsatellites across three major pulse crops. Theoretical and Applied Genetics 110: 1210–1217.Google Scholar
Holton, TA, Christopher, JT, McClure, L, Harker, N and Henry, RJ (2002) Identification and mapping of polymorphic SSR markers from expressed gene sequences of barley and wheat. Molecular Breeding 9: 63–71.Google Scholar
Kantety, R, La Rota, VM, Matthews, DE and Sorrells, ME (2002) Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat. Plant and Molecular Biology 48: 501–510.Google Scholar
Katti, MV, Ranjekar, PK and Gupta, VS (2001) Differential distribution of simple sequence repeats in eukaryotic genome sequences. Molecular Biology and Evolution 18: 1161–1167.Google Scholar
Liu, K and Muse, SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21: 2128–2129.Google Scholar
Louette, D, Charrier, A and Berthaud, J (1997) In situ conservation of maize in Mexico: genetic diversity and maize seed management in a traditional community. Economic Botany 51: 20–38.Google Scholar
Peakall, R and Smouse, PE (2006) GENALEX6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295.Google Scholar
Pritchard, JK, Stephens, M and Donnelly, P (2000) Inference of population structure using multilocus genotype data. Genetics 155: 945–959.Google Scholar
Schuelke, M (2000) An economic method for the fluorescent labeling of PCR fragments. Nature Biotechnology 18: 233–234.Google Scholar
Tadesse, W and Bekele, E (2003) Phenotypic diversity of Ethiopian grass pea (Lathyrus sativus L.) in relation to geographical regions and altitudinal range. Genetic Resources and Crop Evolution 50: 497–505.Google Scholar
Van Oosterhout, C, Hutchinson, WF, Wills, DPM and Shipley, P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4: 535–538.CrossRefGoogle Scholar
Yadav, SS and Bejiga, G (2006) In: Brink, M and Belay, G (eds) Lathyrus sativus L. Record from Protabase. Wageningen: PROTA (Plant Resources of Tropical Africa), http://database.prota.org/search.htm.Google Scholar
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