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SCAR markers of the R-genes and germplasm of wild Solanum species for breeding late blight-resistant potato cultivars

Published online by Cambridge University Press:  15 March 2011

Ekaterina Sokolova ,
Artem Pankin ,
Maria Beketova ,
Maria Kuznetsova ,
Svetlana Spiglazova ,
Elena Rogozina ,
Isol'da Yashina  and
Emil Khavkin
Ekaterina Sokolova
Affiliation:
Institute of Agricultural Biotechnology, Moscow, Russia
Artem Pankin
Affiliation:
Institute of Agricultural Biotechnology, Moscow, Russia
Maria Beketova
Affiliation:
Institute of Agricultural Biotechnology, Moscow, Russia Institute of Potato Husbandry, Korenevo, Russia
Maria Kuznetsova
Affiliation:
Institute of Phytopathology, Bol'shiyeVyazemy, Russia
Svetlana Spiglazova
Affiliation:
Institute of Phytopathology, Bol'shiyeVyazemy, Russia
Elena Rogozina
Affiliation:
Vavilov Institute of Plant Industry, St.-Petersburg, Russia
Isol'da Yashina
Affiliation:
Institute of Potato Husbandry, Korenevo, Russia
Emil Khavkin*
Affiliation:
Institute of Agricultural Biotechnology, Moscow, Russia
*
*Corresponding author. E-mail: emil.khavkin@gmail.com
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Abstract

New races of Phytophthora infestans rapidly defeat potato late blight (LB) resistance based on Solanum demissum germplasm, and breeders search for new sources of durable LB resistance. We developed and verified six sequence characterized amplified region markers recognizing the race-specific genes R1 and R3 of S. demissum and the broad-spectrum resistance gene RB of S. bulbocastanum and the germplasms of these species and used them to screen 209 accessions of 21 wild Solanum species. In addition to S. demissum, homologues of R1 and R3 were found in several species of series Demissa,Longipedicellata and diploid Tuberosa; R3 homologues were also detected in S. bulbocastanum,S. cardiophyllum and S. ehrenbergii. The RB homologues were found in a wider range of Solanum species. The markers of R1 and R3 genes reliably discerned between germplasms of S. tuberosum ssp. tuberosum and wild sources of LB resistance. Following introgression, the species-specific markers of demissum and bulbocastanum germplasm were rapidly lost, whereas the markers of R1 and R3 genes lasted through several meiotic generations and were maintained at high frequencies in modern potato cultivars. The presence of these markers in demissoid potato cultivars was significantly associated with LB resistance, presuming that both genes contribute to overall defence response.

Keywords

allele miningCC-NBS-LRR geneslate blight resistanceSolanum spp.
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 309 - 312
Copyright
Copyright © NIAB 2011

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