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Collection of mutants for functional genomics in the legume Medicago truncatula

Published online by Cambridge University Press:  24 May 2011

O. Calderini ,
M. Carelli ,
F. Panara ,
E. Biazzi ,
C. Scotti ,
A. Tava ,
A. Porceddu  and
S. Arcioni
O. Calderini*
Affiliation:
CNR – Istituto di Genetica Vegetale, Perugia, Italy
M. Carelli
Affiliation:
C.R.A. – Centro per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy
F. Panara
Affiliation:
CNR – Istituto di Genetica Vegetale, Perugia, Italy
E. Biazzi
Affiliation:
C.R.A. – Centro per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy
C. Scotti
Affiliation:
C.R.A. – Centro per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy
A. Tava
Affiliation:
C.R.A. – Centro per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy
A. Porceddu
Affiliation:
Dipartimento Di Scienze Agronomiche e Genetica Vegetale Agraria, Università degli Studi di Sassari, Sassari, Italy
S. Arcioni
Affiliation:
CNR – Istituto di Genetica Vegetale, Perugia, Italy
*
*Corresponding author. E-mail: ornella.calderini@igv.cnr.it
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Abstract

We have established mutant collections of the model species Medicago truncatula according to current protocols. In particular, we used a transposon (Tnt1) tagging method and an ethyl methanesulfonate (EMS) mutagenesis approach (TILLING). The collections were subjected to both forward and reverse genetics screenings, and several mutants were isolated that affect plant traits (e.g. shoot, root developments, flower morphology, etc.) and also biosynthetic pathways of secondary compounds (saponins and tannins). Genes responsible for some of the mutations were cloned and further characterized.

Keywords

Medicago truncatulamutantsTransposon taggingTILLING
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 174 - 176
Copyright
Copyright © NIAB 2011

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