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Biomineralization in human pancreas: A combined infrared-spectroscopy, scanning electron microscopy, x-ray Rietveld analysis, and thermogravimetric study

Published online by Cambridge University Press:  21 December 2015

Samiran Pramanik ,
Soumen Ghosh ,
Arkaprovo Roy  and
Alok Kumar Mukherjee
Samiran Pramanik
Affiliation:
Department of Physics, Jadavpur University, Kolkata, West Bengal 700032, India
Soumen Ghosh
Affiliation:
Department of Physics, Gour Mahavidyalaya, Malda, West Bengal 732142, India
Arkaprovo Roy
Affiliation:
Department of Surgery, Malda Medical College and Hospital, Malda, West Bengal 732101, India
Alok Kumar Mukherjee*
Affiliation:
Department of Physics, Jadavpur University, Kolkata, West Bengal 700032, India
*
a) Address all correspondence to this author. e-mail: akm_ju@rediffmail.com
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Abstract

Structural characterization, quantitative phase analysis, and morphological behavior of biomineralized deposits in human pancreas [pancreatic stones (PSs)] have been carried out using infrared (IR)-spectroscopy, scanning electron microscopy (SEM), powder x-ray diffraction, and thermogravimetry - differential scanning calorimetry (TG–DSC). The fourier transform infrared (FT-IR) spectra indicated that the primary composition of PSs was calcium carbonate. An x-ray powder diffraction phase quantification using the Rietveld method revealed that five of the pancreatic calculi were composed exclusively of calcite (CAL) and the remaining four contained small amounts of vaterite and aragonite in addition to the CAL phase. The crystallite size of CAL in the PSs study varied between 104(6) and 181(2) nm. The SEM images of pancreatic calculi showed a variety of crystal morphologies for biogenic CAL crystallites such as, thin plates, spherulites, prisms, and cylindrical laths. Thermogravimetric analysis of PS1 reveals that biogenic CAL is stable up to 910 K, above which temperature CAL transforms into calcium oxide.

Keywords

pancreatic stonesx-ray powder diffractionscanning electron microscopyquantitative phase analysisRietveld method
Type
Biomineralization and Biomimetics Articles
Information
Journal of Materials Research , Volume 31 , Issue 3 , 15 February 2016 , pp. 328 - 336
Copyright
Copyright © Materials Research Society 2015 

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Footnotes

Contributing Editor: Colin Freeman

References

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