Ion pumps create ion gradients across cell membranes while consuming light energy or chemical energy. The ion gradients are used by the corresponding cell types for passive-ion transport via ion channels or carriers or for accumulation of nutrients like sugar or amino acids via cotransport systems or antiporters.

Proteins exposed to ionizing radiation suffer both reversible and irreversible effects. Reversible effects are defined as those which disappear in a short period of time after the removal of the radiation field and without further treatment of the sample. Irreversible effects are those which cause a permanent alteration in the structure of a protein.

Macromolecular structure determination by X-ray crystallography and solution NMR spectroscopy has experienced unprecedented growth during the past decade.