According to animal breeding theory, profit after, say, 10 generations of selection is maximized when the usually non-linear profit function is approximated by a linear breeding goal where the linearization is at the population mean in generation 10 and the linear breeding goal is subsequently predicted by a linear index for which the animals are selected. The prediction of the population mean at generation 10 requires linear relationships among the traits that constitute the non-linear profit, because otherwise this prediction becomes very complicated.

A non-linear index is proposed that simply estimates the non-linear goal H =f(u) by Ĥ =f(û), where u = vector of genetic values for the traits and u is its (BLUP) estimate. This non-linear index does not require predictions of (future) population means and does not require linearly related traits.

To test these indices a simple meat production example was constructed where the non-linearity between the traits was due to the competition between weight and probability of survival for the same resources from food intake. In the model selection for weight and, in particular, for weight over costs (mainly food) led to reduced profits due to large reductions of survival rates. Although, the example was oversimplified, this should provide a warning for the use of oversimplified breeding goals, e.g. fitness traits may reduce by more than expected from base population genetic parameters.

When probability of survival and weight were measured, a non-linear index of these non-linear traits gave the greatest genetic gains. Failure to update genetic parameters each generation severely reduced genetic gain and, if linear indices were used, it was also important to update the economic weights. When probability of survival was measured, profit could be calculated on each animal and included as a trait in the calculation of estimated breeding value. This yielded high genetic gain and did not require updating of genetic parameters or economic weights.

The relationship between the interval to post-partum (PP) commencement of luteal activity (CLA) and traditional measures of fertility was studied in 1733 lactations of British Friesian cows. Milk progesterone (P4) concentrations were used to determine the interval to PP ovulation and to confirm pregnancy. Milk samples were taken thrice weekly from cows in 20 commercial herds and daily in the University of Nottingham research herd. Commencement of luteal activity was defined as the day of first PP occurrence ofP4 levels >3 μg/l which was then maintained for at least two consecutive readings in the commercial herds and for 4 days in the university herd. Records from animals with retained placenta, uterine infection or those treated with reproductive hormone preparations for reproductive disorders were excluded for this analysis from the original database of cows during 2503 lactations. The interval to PP CLA averaged 27·0 (s.d. 12·1) days (no. = 1733), interval to first service 71·2 (s.d. 19·9) days (no. = 1646), interval to conception 87·2 (s.d. 35·0) days (no. = 1510), number of services per conception 1·50 (s.d. 0·83, no. = 1510) and conception to first service was 0·65. Linear models were then fitted to subsets of the data with complete information to assess the influences of lactation number, season and herd (at least 1243 observations in all analyses). The interval to PP CLA was favourably correlated with measures of fertility such that for every day delay in the interval to CLA there was an average delay of 0·24 and 0·41 days (P < 0·001) in the interval to first service and conception, respectively. The number of services per conception was reduced by 0·11 for each additional 21 days in the interval between PP CLA and first service (P < 0·001). Furthermore, conception rate was influenced by the interval from the onset of luteal activity to first insemination (P < 0·01) such that each additional 21 days progressively reduced the probability of failure at a given insemination number to 0·89 of its previous value. There were significant effects of parity and season (P < 0·05) on the interval to first PP service while the variation among herds in the interval to PP service and days open was significant (P < 0·01). The regressions of measures of fertility on milk yield were not significant. This extensive database on the hormone profiles of PP cows shows that the early re-establishment of PP ovarian activity is an important prerequisite for high fertility.

Forty-five Bos indicus (zebu) cows were blocked by body condition (BC) score at calving and allocated by body weight (BW) to three treatments: untreated controls (CON), and progesterone-releasing intra-vaginal device (PRID)-treated at either 45 (T45) or 60 days (T60) post partum. Cows were given food individually and blood-sampled three times a week for progesterone. Calves sucked twice a day and were weighed weekly. PRIDs resulted in only 21% of cows responding at 60 days and they did not reduce anoestrus (PPI) or calving interval (CI) (P > 0·05). Dry matter (DM) and metabolizable energy (ME) intake were 7·1 (s.e. 0·6) kg and 54·3 (s.e. 4·6) MJ/day, respectively. BC score and BW at calving, and ME intake influenced cow and calf average daily gain ranging from −580 to 625 and 331 to 868 g/day, respectively. Milk yield during the first 4 months was 507 (s.e. 97) l. Cows gained 25 kg per unit increase in condition but lost 50 kg for a unit decline. CI (mean 14·2, range 10 to 24 months) was inversely related to BC score at calving (r = −0·31) and was delayed by a long PPI (r = 0·51), ranging from 38 to 297 days. Most (95%) cows experienced one or more ‘silent ovulations’ before first oestrus, the number extending PPI length (r = 0·49, P < 0·001). Efficiency of ME utilization was higher for smaller cows of 240 (s.e. 28) kg at calving. It is concluded that: (1) PRID treatment before 60 days post partum is unlikely to reduce anoestrus in Boran zebu cattle; (2) given adequate energy intake zebu cattle in thin condition at calving initially replenish body reserves at the cost of lactation and experience more ‘silent’ ovarian activity before returning to oestrus; (3) cows in good condition at calving express maximum lactation potential, mobilizing their own body reserves to support milk production, yet resume oestrous activity earlier despite weight losses to support higher milk yields.

Milk temperature was measured automatically during milking with a view to detecting silent oestrus in dairy cows. A total of 38 silent oestrous periods were studied, based mainly on ovulation and milk progesterone levels together with visual observation.

In 78·9% of silent oestrous periods, a significant increase in milk temperature amounting to a mean of 0·6 (s.e. 0·07)°C was found, and this was highly correlated (r = 0·90) with an increased body temperature. In most silent oestrous periods increases in milk and body temperatures and ovulation with milk progesterone levels below 2 μg/l were found, the oestrous cycles being characterized by typical progesterone profiles. In some cows increases in milk and body temperatures and ovulation during a silent oestrous period were associated with non-typical progesterone profiles although most of these occurred shortly after parturition.

The herd life of dairy cows was approximated by a single lifespan value based on the geometric distribution and accounting for the number of lactations each cow had completed or, if still in the herd, was expected to complete. For cows which had completed lactation n, but had not had time to complete lactation n + 1, the probability of survival from lactation n to complete lactation n + 1, from n + 1 to n + 2 etc. was incorporated, utilizing average population survival probabilities.

Formulae for the genetic variance in lifespan, its heritability and the genetic regression of breeding value on phenotype, the latter found to be independent of the amount of information included in the phenotype, are derived and checked by simulation.

From an analysis of 25227 dairy cows, each with the opportunity to complete five lactations, the heritability of lifespan was 0·06 and its genetic correlation with survival to complete three, four and five lactations ranged from 0·90 to 0·96, for both complete and incomplete survival information, showing that the lifespan trait appears to account adequately for missing observations. Genetic correlations between lifespan and first lactation yield traits were around 0·50, whilst the corresponding phenotypic correlations were approximately 0·13.

To determine a best measure for predicting genetic merit for milking and nursing ability in beef cattle, restricted maximum likelihood (REML) estimates of variances and covariances for direct and maternal effect were obtained by using pre-weaning growth records of calves at various stages after parturition until weaning in Japanese Black cattle. Data used in this study were pre-weaning growth records of 1892 Japanese Black calves obtained from the Tottori National Livestock Breeding Station covering the period from 1960 to 1985. Traits analysed were adjusted monthly weights, adjusted monthly heights and cumulative daily gains (DGs) from birth to 1, 2, 3, 4, 5 and 6 months of age. Estimates of variances and covariances were obtained by REML using the DFREML program of Meyer. Results indicate that: (1) the direct effects on pre-weaning growth of calves are the smallest at birth to 1 month of age and then increase; (2) the maternal effects of the dams are the largest for the first 2 months, and then decrease; (3) the contribution of these two effects on calf growth is reversed at 3 or 4 months of age; (4) the non-additive maternal effects exist but are small; and (5) the genetic correlations between direct and maternal effects are low for weight and cumulative DG, whereas those for height are relatively high. These results suggest that DGfrom birth to 2 months of age (DGO-2) is most influenced by the additive maternal effect. Thus, DGO-2 is concluded to be the best measure for predicting genetic merit for milking and nursing ability in beef cattle.

The aim of the current study was to investigate the effects of level of feeding and level offish-meal supplementation on the carcass composition of young steers and in doing so, to assess the potential for employing near infrared reflectance spectroscopy (NIRS) in such studies. In addition to wet chemical techniques, NIRS was used to examine carcass samples from animals offered silage-based diets at one of four levels of feeding ranging from near maintenance to ad libitum and with one of four levels offish meal (0, 50,100 or 150 g/kg silage dry matter).

Wet chemical data indicated an increase in fat concentration (P < 0·001) and decrease in crude protein concentration (P < 0·05) in the fresh carcass in response to increasing level of feeding but no statistically significant effect of level of fish meal. Ash concentration was not affected significantly by either level of feeding or level of fish-meal supplementation. Ground, freeze-dried samples were scanned in the wavelength range 1100 to 2498 nm. Calibration equations for ash, fat and crude protein concentration (g/kg carcass) were derived using a modified partial least-squares regression technique. Equations were found to be superior for fat compared with those for crude protein and ash. Standard errors of calibration (g/kg carcass) and multiple correlation coefficients of 6·96 and 0·42, 6·61 and 0·95 and 4·36 and 0·61 were obtained for ash, fat and crude protein respectively with corresponding standard errors of cross validation of 7·71, 7·82 and 4·96 g/kg carcass respectively. Qualitative analysis of spectral information using multivariate techniques and difference spectra clearly showed differences in carcass composition resulting from the different levels of feeding and less so the different levels offish-meal supplementation.

It is shown, that NIRS can be used both quantitatively and qualitatively to study the effects of nutrition on carcass composition.

Experiment 1, with rams (17·0 kg initial weight (M)), and experiment 2, with steers (203 kg M), involved 2 × 2 arrangements of treatments to compare the effect of doubling the amount of stover offered (25 or 50 g/kg M daily) and chopping (unchopped or chopped), upon intake, selection and live-weight change. The stover used was a non-bird-resistant, local variety (Dinkamash). The particle length distribution of the chopped stover (produced by a tractor-driven chaff cutter) was: <4·5 mm, 0·148; 4·5 to 8 mm, 0·157; 8 to 12 mm, 0·181; 12 to 20 mm, 0·269; 20 to 33 mm, 0·168; 33 to 54 mm, 0·040; 54 to 90 mm, 0·003; >90mm, 0·034. The stover was supplemented with minerals and cottonseed cake (sheep, 0·1 kg dry matter (DM) per day; cattle, 0·76 kg DM per day). Experiment 1, over 56 days, involved 48 Menz Highland, 18-month-old, rams, with four replicate pens containing three rams. Experiment 2, over 49 days, used 32, individually penned, Friesian × zebu yearling steers. With rams, doubling the amount offered and chopping increased intake, and the effects were additive (unchopped: 0·98 v. 2·24; chopped: 1·08 v. 1·60 (s.e. 0·071) kg DM per pen per day). With steers, there was an amount × chopping interaction (P < 0·05) (unchopped: 3·7 v. 4·7 chopped: 3·6 v. 3·9 (s.e. 0·16) kg DM per day), with chopping reducing intake of stover offered at 50 g/kg M per day. Increased intakes were associated with increased growth rate. In both experiments refused stover contained less leaf-plus-sheath than offered stover but the difference was more pronounced in rams compared with steers indicating the superior selective ability of the rams. In rams offered unchopped stover, the increase in stover intake with increasing amount offered was accounted for by a proportional 1·03 unit increase in consumption of leaf-plus-sheath and a 0·06 unit reduction in intake of stem. Doubling the amount of unchopped stover offered increased intake of both rams and steers by 0·27 unit. Chopping stover was clearly beneficial for rams but not for steers. Doubling the amount of stover offered is a simple excess-feeding strategy to apply. Excess feeding also increases the proportion of stover refused from about 0·2 to 0·5, thus generating an uneaten residue available for other purposes, e.g. mulch or compost.

The relationships between body weight (BW), body condition score (BCS) and internal fat deposits were studied in Latxa dairy ewes. In 2 years, the BW and BCS of 20% of the ewes in 14 flocks were recorded at the beginning of the mating period (pre-mating), and in the 1st month of lactation (post lambing). BW increased with age (BW = 54·05 × (1-e−0·988 × age), and the mature weight was not reached until 4 years, after which weight did not differ between pre-mating and post-lambing periods. Body condition score, however, differed between the two periods. The regression coefficients (b) of BW on BCS, when flock-year and age were included in the regressions, differed significantly (P < 0·05) in the pre-mating and post-lambing periods, and were 5·3 (s.e. 0·31) and 6·3 (s.e. 0·43) kg per unit BCS, respectively. The correlation coefficients (r) were 0·77 and 0·71 respectively, compared with 0·42 and 0·38 when the flock-year and age of the ewes were not included in the regression.

Thirty-seven of these ewes, with BCS between 0·75 and 4·00, were slaughtered, the weights of omental, mesenteric and kidney and channel fat recorded, and the lumbar fat thickness assessed at the last rib. The regressions between the weight of fat deposits and lumbar fat thickness and BW and BCS fitted to semi-logarithmic equations, except for mesenteric fat, in which the relationship was linear. Body condition score was a better predictor than BW for individual fat deposits, with proportionately 0·81 to 0·83 of the variation in the weight of the deposits accounted for by variation in BCS and 0·57 to 070 by variation in BW.

The objective of this study was to compare the effects of melatonin implants combined with intravaginal, progestagen-impregnated pessaries with the conventional treatment of progestagen pessaries followed by pregnant mare serum gonadatrophin (PMSG) on the reproductive performance of ewes during the anoestrous period.

A commercial flock of 179 seasonally anoestrous Chios crossbred ewes was used. The ewes within the flock were randomly allocated to one of two groups. Ewes of group MP (melatonin + pessaries) received melatonin implants on 20 March (50 days before the target breeding date of 10 May) and were treated with intravaginal progestagen pessaries 35 days later (25 April). Ewes of group PP (pessaries + PMSG) were also treated with pessaries on 25 April. After 14 days the pessaries were removed from both groups and 500 i.u. PMSG was injected (i.m.) into ewes of group PP only. Fertile rams were introduced into both groups 24 h after sponge removal. Pregnancy rate and litter size were determined by ultrasound scanning 80 days after ram introduction.

Pregnancy rate at first oestrus was significantly higher in group MP than in group PP (57 v. 40%, P < 0·05). The overall conception rate during two oestrous cycles was significantly higher in group MP than in group PP (78 v. 59%, P < 0·02). The groups did not differ significantly in the mean number of foetuses per pregnant ewe that conceived during the first or second oestrus after pessary removal (1·78 and 1·87, respectively). The total number of foetuses per treated ewe was higher in group MP than in group PP (1·39 v. 1·10) as a consequence of the difference in pregnancy rate.

The effect of lambing date on the subsequent onset and duration of ovarian cyclicity in Mule (Bluefaced Leicester × Scottish Blackface) ewes was investigated. Nineteen ewes which had lambed in January (16 January 1993 (s.e. 3 days)) and been weaned in February-March and 22 comparable ewes which had lambed in May (14 May 1993 (s.e. 2 days)) and been weaned on 23 August were maintained at pasture as two isolated groups. A raddled vasectomized ram was continually present with each group from 14 July 1993 to 26 May 1994 and marked (oestrous) ewes were recorded twice weekly. Ovarian activity was assessed by measuring peripheral progesterone concentrations in blood samples collected twice weekly and by laparoscopic viewing of the ovaries of all ewes during October, January and March. The onset and duration of ovarian activity were significantly affected by the previous lambing date. For January and May lambing ewes, mean dates of onset were 5 September 1993 (s.e. 2 days) v. 25 September 1993 (s.e. 4 days) (P < 0·001) and of cessation were 5 April 1994 (s.e. 5 days) v. 10 April 1994 (s.e. 3 days). Mean durations of ovarian activity were 212 (s.e. 6) and 195 (s.e. 5) days (P < 0·05) during which 12·4 (s.e. 0·29) and 11·5 (s.e. 0·38) ovarian cycles respectively were recorded. Ovulation rate was not affected by previous lambing date but was significantly lower in March compared with October (January lambing ewes 1·7 (s.e. 0·1) v. 2·3 (s.e. 0·1) (P < 0·001); May lambing ewes 1·6 (s.e. 0·1) v. 2·1 (s.e. 0·1) (P < 0·01)). Results demonstrate that (i) Mule ewes have a potential breeding season of up to 8 months duration; (ii) the onset and duration of ovarian activity can be influenced by previous lambing date; and (Hi) a seasonal decline in ovulation rate may, in practical terms, result in a lower lambing percentage for animals bred towards the end of their natural breeding period.

Four adult ewes (mean weight 42·6 kg) fitted with oesophageal fistulae were given 5 mmol/day ofallantoin or saline solutions by intrajugular continuous infusion. The experiment was a randomized cross-over design, with two consecutive 3-day infusion periods. One kg/day fresh matter of either chopped or pelleted fescue hay was distributed over 12 meals and salivary flow estimated from dilution of Co-EDTA infused into the buccal cavity. Allantoin infusion resulted in a rapid increase in its plasma concentration (84 to 128 (s.e. 1·5) μmol/l) and urinary excretion (9·6 to 13·3 (s.e. 0·18) mmol/day) without significant differences between diets. Salivary allantoin also increased (4·6 to 6·4 (s.e. 0·60) ymol/1) in response to infusion, although the concentration of total purine derivatives in saliva was only proportionately 0·08 that of plasma. Renal and salivary clearance of oxypurines, allantoin (78 (s.e. 5·0) ml/min and 13 (s.e. 0·7) ml/h), uric acid (466 (s.e. 98·0) ml/min and 45 (s.e. 9·8) ml/h) and creatinine (104 (s.e. 3·0) ml/min and 14 (s.e. 1·1) ml/h) were constant, irrespective of diet and infusion treatments. Urinary recovery of infused allantoin averaged 0·78 (s.e. 0·031) but salivary secretion, equivalent to about 0·003 of urinary losses, was not the explanation for the incomplete recovery.

A selection experiment was established in Coopworth sheep in 1981 to breed for increased or reduced ultrasonic backfat depth (scan C). Foundation females came from four flocks recorded for scan C and live weight, with weight-adjusted scan C data within flock being used for initial screening and subsequent selection. Three groups of animals per source flock, comprising proportionally the fattest 0·12, a random sample, and the leanest 0·12, were used to establish the F, control and L lines, respectively. Ewe flock numbers from 1981 to 1992 averaged 51 per line. Foundation rams were selected in the same manner from four different farms (two sources each in 1981 and 1982) to provide F line (proportionally the fattest 0·04), control line and L line (leanest 0·04) rams for use in 1981 and 1982, with four mated per line per year. Thereafter homebred rams were selected, with 41 or 42 homebred rams being used per line until 1992. Average generation intervals were 2·13 years and annual inbreeding rates per line 0·004. Bivariate heritability estimates for log scan C and log live weight, and a univariate estimate for log scan C using restricted maximum likelihood with an animal model, were 0·28, 0·22 and 0·38, respectively (all with s.e. 0·03). There was a suggestion of lower heritabilities in the L line for log scan C after adjustment for live weight. Realized heritabilities in the F and L lines were 0·34 (s.e. 0·07) and 0·26 (s.e. 0·03), respectively. Deviations of back-transformed weight-adjusted scan C in the last 2 years ofF and L data analysed (1991 and 1992 birth years) from the control flocks were 2·08 and −0·85 mm, which corresponded to responses of +2·50 and −1·03 phenotypic standard deviations, respectively. In addition there were relatively large responses in live weight taken at scanning, with F and L lines averaging 34·0 and 40·2 kg, compared with 38·0 kg for controls in the 1991 and 1992 birth years. The genetic and phenotypic correlations between log scan C and log live weight at scanning were 0·16 (s.e. 0·07) and 0·46 (s.e. 0·01) respectively.

Shearing ewes during pregnancy frequently increases the birth weight of lambs. In housed ewes, the birth weight responses are greatest when shearing occurs relatively early in pregnancy. This study examined the effects of the timing and method of shearing on foetal growth, as measured by birth weight, in ewes at pasture. Border Leicester × Romney ewes were allocated at day 50 of pregnancy to: four ‘time of shearing’ treatments — pregnancy day 70 (P70), P100, P130 (no. = 60 for each treatment) and unshorn (no. = 34); two methods of shearing (by standard comb or cover comb); and two levels of pregnancy/rearing rank All ewes had been mated over a 14-day period and grazed pasture together throughout the trial The mid-point of lambing was 17 August.

At no time during pregnancy or lactation did ewe live weight differences between the groups exceed 4 kg. Ewes shorn by cover comb were heavier prior to lambing and during lactation than standard comb-shorn ewes. Time of shearing, method of shearing and ewe rearing rank had no effect on ewe annual greasy fleece production. There was a highly significant (P < 0·001) interaction between the effects of birth rank and time of shearing on lamb birth weight. Time of shearing did not affect birth weight of single-born lambs but birth weight of twin lambs increased with earlier maternal shearing to a maximum of 0·7 kg (per lamb) in those born to ewes shorn on P70. These results imply that shearing reduced the greater extent of maternal constraint which applies to the growth of twin v. single foetuses in utero. This selective increase in birth weights of twins achieved by early pregnancy shearing may have advantages in reducing lamb mortality.

The effect of ammonia treatment of straw on both the rumen environment and the extent of its microbial fermentation was studied. Four rumen cannulated sheep were randomly given 700 g/day of untreated straw plus urea (US), ammonia-treated straw (TS) and alfalfa hay (AH) in a change-over design with three periods. Rumen pH was lower and ammonia-nitrogen and total volatile fatty acid (VFA) concentrations were higher (P < 0·001) with AH than with US or TS. With the straw diets, TS promoted a lower pH than US (P < 0·05), but differences were less than 0·3 units and the mean pH was never below 6·5. There were no differences between the straw diets in ammonia-nitrogen or VFA concentration (P > 0·05). When untreated barley straw (BS) and treated straw (TS) were incubated in situ disappearance of dry matter (dDM) at 12, 24 and 48 h (P < 0·01) and neutral-detergent fibre (dNDF) at 48 h (P < 0·001) were higher with TS. In vitro incubation showed a higher gas production with TS only after 36 h (P < 0·05) whereas gas from BS fermentation was higher up to 14 h (P < 0·05). Among diets, dDM, dNDF and gas production with US were numerically higher than with TS or AH throughout, although few significant differences were observed, except for a higher dDM at 12 (P < 0·01) and 24 (P < 0·10) h and a higher dNDF at 12 h (P < 0·10). Particle-associated enzymes were extracted from BS and TS incubated in the rumen for 4, 8, 22 and 24 h. Results ofxylanase and cellulase activities support those of straw incubation, with a drop between 4 and 8 h in TS diet. The concentration of residual phenolics per unit of incubated straws after 12 and 24 h show that phenolics release to the media was higher with the TS diet. Daily changes of phenolic concentration into rumen liquid was also higher with TS than with US (P < 0·001). The increased release of straw phenolics by ammoniation reduced the potential for rumen degradation of straw, mainly in the first hours of the fermentation period.

A study was carried out to compare the fermentation parameters and kinetics of digestion of a range of different foods in the rumen of two breeds of sheep (Churra and Merino). Ten mature sheep (five Churra and five Merino), each fitted with a rumen cannula, were used in this study. In situ rumen degradability of both dry matter (DM) and cell wall was greater in Churra than in Merino sheep, the breed differences being significant for most of the foods used in the study (P < 0·05). These differences were greater when the foods had a higher cell wall concentration and this could be related to differences in the ruminal environment. However, when the foods were incubated with rumen fluid their in vitro organic matter (OM) degradability was similar in both breeds. Rumen pH was higher (P < 0·05) and ammonia concentrations were lower (P < 0·05) in Churra than in Merino sheep. Rumen volatile fatty acid concentrations tended to be higher in Merino than in Churra sheep, though differences were only significant just before feeding (P < 0·05). The ratio acetate: propionate was higher in the Churra than Merino breed before and 12 h after feeding (P < 0·05). Protozoa numbers in rumen liquid were similar for both genotypes. The greater degradation of forages in the rumen of Churra sheep is discussed in relation to the possible higher activity of fibre-degrading micro-organisms and the greater buffering capacity of the rumen contents against fermentation acids, which could result in more favourable conditions for the microbial degradation of foods in the rumen.

Feeding behaviour of six sows receiving a diet with a high inclusion (500 g/kg) of sugar-beet pulp (SBP) was compared with that of six sows receiving a conventional cereal-based diet (control). The feeding rate was determined for both the rate of consumption of the daily food allowance (SBP or control diet) and of a test portion (of control diet) offered at different intervals after the daily food allowance. SBP sows consumed their daily food allowance more slowly than control sows, SBP sows also consumed the test portion more slowly than control sows, when this was offered immediately after the daily food allowance. Prolonging the time taken to eat the control diet, by presenting it in meal form, did not change the feeding rate of the test diet. Offering the test portion 2 h after the daily food allowance, increased the feeding rate of the test portion for both control and SBP sows, with a smaller but still significant treatment difference. Gastric distension appeared to be a major factor in regulating intake when feeding a diet containing SBP. The extent to which feeding rate could be used as an index of feeding motivation and the welfare consequences of using bulky diets for sows are discussed.

Employing practical-type diets containing graded levels of all-rac-α-tocopheryl acetate (0, 5, 15, 30, 45, 60, 75 or 100 mg/kg), the quantitative requirement for a-tocopherol by juvenile African catfish (Clarias gariepinus Burchell) was investigated. Diets, defined with respect to selenium, fatty acid and ascorbate inclusion, were offered for 12 weeks during which catfish grew from 10 g to 120 g. Dietary regime had no significant effect on growth (P > 0·05), although haematocrit values increased with increasing tocopherol dose above 15 mg/kg (assayed) (R2 = 0·71, P < 0·05). Tissue accretion of the vitamin increased concomitant with dietary dose (P < 0·001). Employing a colorimetric assay for thiobarbituric acid reactive substances (TBARS) it was evident that concentration ofTBARS in muscle and liver reflected tissue oxidation status, in that elevated dietary vitamin E resulted in decreased assayed TBARS (P < 0·05). By broken-line analysis the requirement for this species to suppress lipid peroxidation was estimated as 30 to 40 mg all-rac-a-tocopheryl acetate per kg dry diet and this agrees with published requirements of many fish species.