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In vitro activity of ‘Mexican Arnica’ Heterotheca inuloides Cass natural products and some derivatives against Giardia intestinalis

Published online by Cambridge University Press:  03 November 2014

JOSÉ LUIS RODRÍGUEZ-CHÁVEZ ,
YADIRA RUFINO-GONZÁLEZ ,
MARTHA PONCE-MACOTELA  and
GUILLERMO DELGADO
JOSÉ LUIS RODRÍGUEZ-CHÁVEZ
Affiliation:
Instituto de Química, Universidad Nacional Autónoma de México, 04510 México, D. F., México
YADIRA RUFINO-GONZÁLEZ
Affiliation:
Laboratorio de Parasitología Experimental, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, 04530 México, D. F., México
MARTHA PONCE-MACOTELA
Affiliation:
Laboratorio de Parasitología Experimental, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, 04530 México, D. F., México
GUILLERMO DELGADO*
Affiliation:
Instituto de Química, Universidad Nacional Autónoma de México, 04510 México, D. F., México
*
*Corresponding author. Instituto de Química, Universidad Nacional Autónoma de México, 04510 México, D. F., México. E-mail: delgado@unam.mx
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Summary

Giardiasis is a gastrointestinal disease that affects humans and other animals caused by parasitic protists of the genus Giardia. Giardia intestinalis (Syn. Giardia lamblia; Giardia duodenalis) infections can cause acute or chronic diarrhoea, dehydration, abdominal discomfort and weight loss. Metronidazole is the most widely used drug for treating giardiasis. Although effective, metronidazol has undesirable secondary effects. Plants used in traditional medicine as antidiarrhoeals or antiparasitics may represent alternative sources for new compounds to treat giardiasis. Heterotheca inuloides Cass. (Asteraceae/Compositae) plant is widely used in Mexican traditional medicine. The following secondary metabolites were isolated from H. inuloides flowers: 7-hydroxy-3,4-dihydrocadalene (1), 7-hydroxycadalene (2), 3,7-dihydroxy-3(4H)-isocadalen-4-one (3), 1R,4R-hydroxy-1,2,3,4-tetrahydrocadalen-15-oic acid (4), quercetin (5), quercetin-3,7,3′-trimethyl ether (6), quercetin-3,7,3′,4′-tetramethyl ether (7) and eriodictyol-7,4′-dimethyl ether (8). The activity of these compounds against Giardia intestinalis trophozoites was assessed in vitro as was the activity of the semisynthetic compounds 7-acetoxy-3,4-dihydrocadalene (9), 7-benzoxy-3,4-dihydrocadalene (10), 7-acetoxycadalene (11), 7-benzoxycadalene (12), quercetin pentaacetate (13) and 7-hydroxycalamenene (14). Among these, 7-hydroxy-3,4-dihydrocadalene (1) and 7-hydroxycalamenene (14) were the most active, whereas the remaining compounds showed moderate or no activity. The G. intestinalis trophozoites exposed to compound 1 showed marked changes in cellular architecture along with ultrastructural disorganization. The aim of this study was to evaluate the giardicidal activity of selected H. inuloides metabolites and some semisynthetic derivatives using an in vitro experimental model of giardiasis.

Keywords

Heterotheca inuloidesantigiardial activitycadinanesflavonoids
Type
Research Article
Information
Parasitology , Volume 142 , Issue 4 , April 2015 , pp. 576 - 584
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Copyright
Copyright © Cambridge University Press 2014 

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