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Docosahexaenoic acid-enriched egg consumption induces accretion of arachidonic acid in erythrocytes of elderly patients*

Published online by Cambridge University Press:  09 March 2007

Marianne Payet ,
Mohamed H. Esmail ,
Elisabeth Polichetti ,
Gaël Le Brun ,
Lounès Adjemout ,
Gérard Donnarel ,
Henri Portugal  and
Gérard Pieroni
Marianne Payet
Affiliation:
I.N.S.E.R.M. U476, Faculté de Médecine, 27 Bd Jean Moulin, 13385, Marseille, cedex 05, France
Mohamed H. Esmail
Affiliation:
I.N.S.E.R.M. U476, Faculté de Médecine, 27 Bd Jean Moulin, 13385, Marseille, cedex 05, France
Elisabeth Polichetti
Affiliation:
GLON SANDERS, Pontivy, France
Gaël Le Brun
Affiliation:
GLON SANDERS, Pontivy, France
Lounès Adjemout
Affiliation:
Centre Gérontologique Départemental, Marseille, France
Gérard Donnarel
Affiliation:
Centre Gérontologique Départemental, Marseille, France
Henri Portugal
Affiliation:
I.N.S.E.R.M. U476, Faculté de Médecine, 27 Bd Jean Moulin, 13385, Marseille, cedex 05, France
Gérard Pieroni*
Affiliation:
I.N.S.E.R.M. U476, Faculté de Médecine, 27 Bd Jean Moulin, 13385, Marseille, cedex 05, France
*
†Corresponding author: Dr Gérard Pieroni, fax +33 4 91 78 21 01, email gerard.pieroni@medecine.univ-mrs.fr
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Abstract

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Many studies in humans volunteers have shown that dietary docosahexaenoic acid (DHA) supplied as triacylglycerol can increase DHA levels in blood lipids but often strongly decreases those of arachidonic acid (AA). The aim of the present study was to determine the effect of dietary supplementation with egg-yolk powder enriched in DHA, corresponding to the French recommended dietary allowance for DHA, on the blood lipid status of an elderly population. Institutionalised elderly individuals aged between 63 and 93 years consumed an egg product enriched in DHA (150 mg/d) once daily for 9 months. Plasma lipids and the fatty acid composition of erythrocyte membranes were determined every 3 months. The supplementation induced an increase in the PUFA content of plasma and erythrocyte membranes which was +14·5 and +25·3 %, respectively, at 9 months. This effect was mainly due to the level of DHA and, unexpectedly, to that of AA which continuously increased. This increase in AA was the result of an increased dietary intake (+50 mg/d) and very probably of an increased biosynthesis as demonstrated by the behaviour of di-homo-γ-linolenic acid. The supplementation resulted in a blood PUFA status comparable with that of young healthy controls. The data are consistent with a strong regulatory action of the dietary treatment on the subjects' lipid metabolism.

Keywords

Docosahexaenoic acidArachidonic acidEnriched egg yolkElderly
Type
Research Article
Information
British Journal of Nutrition , Volume 91 , Issue 5 , May 2004 , pp. 789 - 796
Copyright
Copyright © The Nutrition Society 2004

Footnotes

*

Presented as an oral communication at ISSFAL Fifth Congress, 7–11 May 2002, Montreal, Canada.

References

Asciutti-Moura, LS, Guilland, JC, Fuchs, F, Richard, D & Klepping, J (1988) Fatty acid composition of serum lipids and its relation to diet in an elderly institutionalized population. Am J, Clin N, utr 48, 980–987.CrossRefGoogle Scholar
Bernard, C (1995) Préparation de glycérophospholipides enrichis en acide docosahexaénoïque (Preparation of docosahexaenoic enriched glycerophospholipids). Etude de leurs effets chez le lapin néozélandais hypercholestérolémique (Study of their effects on hypercholesterolaemic New Zealand rabbit). PhD thesis, Aix-Marseille III. Université de Droit, d'Economie et des Sciences d'Aix-Marseille III, Marseille.Google Scholar
Bligh, EG & Dyer, WJ (1959) Rapid method of total lipid extraction and purification. Can J, Biochem P, hysiol 57, 911–917.Google Scholar
Brenner, RR (1977) Regulatory function of delta6 desaturate – key enzyme of polyunsaturated fatty acid synthesis. Adv Exp Med Biol 83, 85–101.Google Scholar
Brossard, N, Croset, M, Pachiaudi, C, Riou, JP, Tayot, JL & Lagarde, M (1996) Retroconversion and metabolism of [13C]22:6n-3 in humans and rats after intake of a single dose of [13C]22:6n-3-triacylglycerols. Am J, Clin N, utr 64, 577–586.Google Scholar
Burdge, GC, Jones, AE & Wootton, SA (2002) Eicosapentaenoic and docosapentaenoic acids are the principal products of alpha-linolenic acid metabolism in young men. Br J Nutr 88, 355–363.Google Scholar
Carlson, SE, Cooke, RJ, Rhodes, PG, Peeples, JM & Werkman, SH (1992) Effect of vegetable and marine oils in preterm infant formulas on blood arachidonic and docosahexaenoic acids. J Pediatr 120, S159–S167.CrossRefGoogle ScholarPubMed
Carnielli, VP, Verlato, G, Pederzini, F, Luijendijk, I, Boerlage, A, Pedrotti, D & Sauer, PJ (1998) Intestinal absorption of long-chain polyunsaturated fatty acids in preterm infants fed breast milk or formula. Am J, Clin N, utr 67, 97–103.Google Scholar
Darcet, P & Driss, F (1985) Apports et utilisations des acides gras essentiels chez les personnes âgées (Contibutions and utilisations of essential fatty acids in old people). In L'alimentation des Personnes Âgées 141–148Paris, FranceCERINGoogle Scholar
De Gomez-Dumm, I & Brenner, RR (1974) Oxidative desaturation of alpha-linolenic, linoleic and stearic acids in human liver microsomes. Lipids 10, 315–317.CrossRefGoogle Scholar
Dyerberg, J, Barg, HO, Stoffersen, E, Moncada, S & Vane, JR (1978) Eicosapentaenoic acid and prevention of thrombosis and atheosclerosis?. Lancet 2 (8081) 117–119Google Scholar
Favreliere, S, Perault, MC, Huguet, FDe Javel, D, Bertrand, N, Piriou, A & Durand, G (2003) DHA-enriched phospholipid diets modulate age-related alterations in rat hippocampus. Neurobiol Aging 24, 233–243.CrossRefGoogle ScholarPubMed
Feinberg, M, Favier, JC, Ireland-Ripert, J (1991) Répertoire Général des Aliments. Table de Composition REGAL. Paris, France: Lavoisier.Google Scholar
Gudbjarnason, S, Benediktsdottir, VE & Skuladottir, G (1989) Effects of n-3 polyunsaturated fatty acids on coronary heart disease. Bibl Nutr Dieta 43, 1–12.Google Scholar
Horrobin, D (1997) Essential fatty acid (EFA) metabolism in patients with diabetic neuropathy. Prostaglandins Leukot Essent Fatty Acids 57 256Google Scholar
Kates, M (1972) Techniques in Lipidology: In Laboratory Techniques in Biochemistry and Molecular Biology pp. 351–353 [Work, TS and Work, E, editors]. North Holland: American ElsevierGoogle Scholar
Klepping, J, Guilland, JC, Fuchs, F, Marcer, I, Houard-Malval, M (1990) Recueil de Données sur la Composition des Aliments Neuilly sur Seine, France: Centre d'Étude et d'Information sur les VitaminesGoogle Scholar
Kyle, DJ, Schaefer, E, Patton, G & Beiser, A (1999) Low serum docosahexaenoic acid is a significant risk factor for Alzheimer's dementia. Lipids 34 S245Google Scholar
Laugharne, JD, Mellor, JE & Peet, M (1996) Fatty acids and schizophrenia. Lipids 31 Suppl. S163–S165CrossRefGoogle ScholarPubMed
Lemaitre-Delaunay, D, Pachiaudi, C, Laville, M, Pousin, J, Armstrong, M & Lagarde, M (1999) Blood compartmental metabolism of docosahexaenoic acid (DHA) in humans after ingestion of a single dose of [13C]DHA in phosphatidylcholine. J Lipid Res 40, 1867–1874.CrossRefGoogle ScholarPubMed
Martin, A (2001) Apports Nutritionnels Conseillés pour la Population Française. Paris, France: LavoisierGoogle Scholar
Munro, HN & Danford, DE (1989) Nutrition, Aging and the Elderly. New York: Plenum PressGoogle Scholar
Nelson, GJ, Schmidt, PC, Bartolini, GL, Kelley, DS & Kyle, D (1997) The effect of dietary docosahexaenoic acid on plasma lipoproteins and tissue fatty acid composition in humans. Lipids 32, 1137–1146.Google Scholar
Ohta, A, Mayo, MC, Kramer, N & Lands, WE (1990) Rapid analysis of fatty acids in plasma lipids. Lipids 25, 742–747.Google Scholar
Paganelli, F, Maixent, JM, Duran, MJ, Parhizgar, R, Pieroni, G & Sennoune, S (2001) Altered erythrocyte n-3 fatty acids in Mediterranean patients with coronary artery disease. Int J Cardiol 78, 27–32.Google Scholar
Pawlosky, R, Hibbeln, J, Lin, Y, Salem, N Jr & Burdge, G (2003) Nutrition Discussion Forum. Br J Nutr 90, 993–995.Google Scholar
Peet, M, Murphy, B, Shay, J & Horrobin, D (1998) Depletion of omega-3 fatty acid levels in red blood cell membranes of depressive patients. Biol Psychiatry 43, 315–319.Google Scholar
Pekiner, B & Pennock, JF (1995) Fatty acids in plasma and red blood cell membranes in humans, rats, rabbits and dogs. Biochem Mol Biol Int 37, 221–229.Google Scholar
Rodriguez-Palmero, M, Lopez-Sabater, MCCastellote-Bargallo, AIde la Torre-Boronat, MC, Rivero-Urgell, M (1997) Administration of low doses of fish oil derived N-3 fatty acids to elderly subjects. Eur J, Clin N, utr 51, 554–560.CrossRefGoogle ScholarPubMed
Simopoulos, AP (1991) Omega-3 fatty acids in health and disease and in growth and development. Am J, Clin N, utr 54, 438–463.CrossRefGoogle ScholarPubMed
Song, JH, Inoue, Y & Miyazawa, T (1997) Oxidative stability of docosahexaenoic acid-containing oils in the form of phospholipids, triacylglycerols, and ethyl esters. Biosci Biotechnol Biochem 61, 2085–2088.Google Scholar
Sprecher, H (1992) Long chain fatty acid metabolism. In Polyunsaturated Fatty Acids in Human Nutrition 13–24 [Bracco, U, Deckelbaum, RJ, New York: Raven PressGoogle Scholar
Sprecher, H, Luthria, DL, Mohammed, BS & Baykousheva, SP (1995) Reevaluation of the pathways for the biosynthesis of polyunsaturated fatty acids. J Lipid Res 36, 2471–2477.Google Scholar
Surai, PFMacPherson, ASpeake, BK & Sparks, NH (2000) Designer egg evaluation in a controlled trial. Eur J, Clin Nutr 54, 298–305.Google Scholar
Van Elswyk, ME (1997) Comparison of n -3 fatty acid sources in laying hen rations for improvement of whole egg nutritional quality: a review. Br J Nutr 78 Suppl. 1, S61–S69CrossRefGoogle Scholar
Vidgren, HM, Agren, JJ, Schwab, U, Rissanen, T, Hanninen, O & Uusitupa, MI (1997) Incorporation of n-3 fatty acids into plasma lipid fractions, and erythrocyte membranes and platelets during dietary supplementation with fish, fish oil, and docosahexaenoic acid-rich oil among healthy young men. Lipids 32, 697–705.Google Scholar
Wells, MA & Hanahan, DJ (1969) Phospholipase A from Crotalus adamanteus Venom. Methods in Enzymol 14, 178–184.CrossRefGoogle Scholar
Wensing, AGCL, Mensink, RP & Hornstra, G (1999) Effects of dietary n-3 polyunsaturated fatty acids from plant and marine origin on platelet aggregation in healthy elderly subjects. Br J Nutr 82, 183–191.Google Scholar
Wijendran, V, Lawrence, P, Diau, GY, Boehm, G, Nathanielsz, PW & Brenna, JT (2002) Significant utilization of dietary arachidonic acid is for brain adrenic acid in baboon neonates. J Lipid Res 43, 762–767.Google Scholar