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Stable isotope-labelled vitamin C as a probe for vitamin C absorption by human subjects

Published online by Cambridge University Press:  09 March 2007

Christopher J. Bates*
Affiliation:
MRC Human Nutrition Research, Cambridge, UK
Kerry S. Jones
Affiliation:
MRC Human Nutrition Research, Cambridge, UK
Leslie J. C. Bluck
Affiliation:
MRC Human Nutrition Research, Cambridge, UK
*
*Corresponding author: Dr Christopher J. Bates, fax +44 1223 437515, email chris.bates@mrc-hnr.cam.ac.uk
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Abstract

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Factors affecting absorption of physiological doses of vitamin C in man have not been widely studied, partly because few suitable tools exist to distinguish recently absorbed vitamin C from endogenous vitamin. Stable isotope-labelled vitamin C provides such a tool. Fifteen healthy non-smoking subjects aged 26–59 years were studied. Each received 30 mg L-[1-13C]ascorbic acid orally on two occasions, 3–4 weeks apart. The ascorbate was given alone or with Fe (100 mg as ferrous fumarate) or with red grape juice, which is rich in polyphenols. Blood was collected at frequent intervals for 1 h, and then each hour for a further 3 h. Total concentration of vitamin C was measured fluorometrically and its 13C-isotope enrichment was measured by GC–MS after conversion to volatile trimethylsilyl esters. Peak plasma enrichment occurred within 25–50 min. No kinetic variables were significantly altered by the iron fumarate supplement. Grape juice attenuated vitamin C absorption, reaching significance at the 20 min time point. There were weak correlations between isotope enrichment and body weight or endogenous ascorbate concentration. The increment in total plasma ascorbate was smaller if calculated from isotope enrichment than from vitamin C concentration increase. The dilution pool was much larger than the plasma ascorbate pool. Further studies are needed to resolve these paradoxes. Stable isotope-labelled ascorbate is potentially useful for measurement of vitamin C absorption by human subjects.

Type
Research Article
Copyright
Copyright © The Nutrition Society 2004

References

Atkins, GL, Dean, BM, Griffin, WJ & Watts, RWE (1964) Quantitative aspects of ascorbic acid metabolism in man. J Biol Chem 239, 29752980.CrossRefGoogle ScholarPubMed
Baker, EM, Hodges, RE, Hood, J, Sauberlich, HE & March, SC (1969) Metabolism of ascorbic-1-14C acid in experimental human scurvy. Am J Clin Nutr 22, 549558.CrossRefGoogle ScholarPubMed
Bates, CJ (1997) Bioavailability of vitamin C. Eur J Clin Nutr 51, S28S33.Google ScholarPubMed
Blanchard, J (1991) Effects of gender on vitamin C pharmacokinetics in man. J Am Coll Nutr 10, 453459.Google Scholar
Blanchard, J, Conrad, KA, Mead, RA & Garry, PJ (1990) Vitamin C disposition in young and elderly men. Am J Clin Nutr 51, 837845.CrossRefGoogle Scholar
Blanchard, J, Tozer, TT & Rowland, M (1997) Pharmacokinetic perspectives on megadoses of ascorbic acid. Am J Clin Nutr 66, 11651171.CrossRefGoogle ScholarPubMed
Bluck, LJC & Coward, WA (1997) Peak measurement in gas chromatographic/mass spectrometric isotope studies. J Mass Spectrom 32, 12121218.3.0.CO;2-9>CrossRefGoogle Scholar
Bluck, LJC, Izzard, AP & Bates, CJ (1996) Measurement of ascorbic acid kinetics in man using stable isotopes and gas chromatography/mass spectrometry. J Mass Spectrom 31, 741748.Google Scholar
Bluck, LJC, Jones, KS & Bates, CJ (2002) 2 H and 13 C labelled tracers compared for kinetic studies of ascorbic acid metabolism in man – a factor analytical approach. Rapid Commun Mass Spectrom 16, 879883.CrossRefGoogle Scholar
Clayton, MM & Borden, RA (1943) The availability for human nutrition of the vitamin C in raw cabbage and home-canned tomato juice. J Nutr 25, 349369.CrossRefGoogle Scholar
Clayton, MM & Folsom, MT (1940) A method for the study of the availability for human nutrition of the vitamin C in foods, with an application to the study of the potato. J Home Econ 32, 390395.Google Scholar
Clegg, KM & Morton, AD (1968) The phenolic compounds of blackcurrant juice and their protective effects on ascorbic acid. II. The stability of ascorbic acid in model systems containing some of the phenolic compounds associated with blackcurrant juice. J Fd Technol 3, 277284.Google Scholar
Diaz, M, Rosado, JL, Allen, LH & Garcia, OP (2003) The efficacy of a local ascorbic acid-rich food in improving iron absorption from Mexican diets: a field study using stable isotopes. Am J Clin Nutr 78, 436440.CrossRefGoogle Scholar
Frankel, EN, Bosanek, CA, Meyer, AS, Silliman, K & Kirk, LL (1998) Commercial grape juices inhibit the in vitro oxidation of human low-density lipoproteins. J Agric Food Chem 46, 834838.Google Scholar
Graumlich, JF, Ludden, TM, Conry-Cantilena, C, Cantilena, LR, Wang, Y & Levine, M (1997) Pharmacokinetic model of ascorbic acid in healthy male volunteers during depletion and repletion. Pharmaceut Res 14, 11331139.Google Scholar
Hankes, LV, Jansen, CR & Schmaeler, M (1974) Ascorbic acid catabolism in Bantu with haemosiderosis (scurvy). Biochem Med 9, 244255.Google Scholar
Harper, KA, Morton, AD & Rolfe, EJ (1969) The phenolic compounds of blackcurrant juice and their protective effects on ascorbic acid. III. The mechanisms of ascorbic acid oxidation and its inhibition by flavonoids. J Fd Technol 4, 255267.Google Scholar
Hartzler, ER (1945) The availability of ascorbic acid in papayas and guavas. J Nutr 30, 355365.CrossRefGoogle Scholar
Hawley, EE, Stephens, DJ & Anderson, G (1936) The excretion of vitamin C in normal individuals following a comparable quantitative administration in the form of orange juice, cevitamic acid by mouth and cevitamic acid intravenously. J Nutr 11, 135145.Google Scholar
Hopper, FC & Ayres, AD (1950) The enzymatic degradation of ascorbic acid. Part I – The inhibition of the enzymatic oxidation of ascorbic acid by substances occurring in blackcurrants. J Sci Fd Agric 1, 58.Google Scholar
Hornig, D, Vuilleumier, J-P & Hartmann, D (1980) Absorption of large, single, oral intakes of ascorbic acid. Int J Vit Nutr Res 50, 309314.Google Scholar
Izzard, AP, Bluck, LJC & Bates, CJ (1996) Measurement of stable isotope [13-C] ascorbate using gas chromatography with mass spectrometry (GCMS). Proc Nutr Soc 55, 88A.Google Scholar
Johnston, CS & Luo, B (1994) Comparison of the absorption and excretion of three commercially available sources of vitamin C. J Am Diet Assoc 94, 779781.CrossRefGoogle ScholarPubMed
Jones, E & Hughes, RE (1984) The influence of bioflavonoids on the absorption of vitamin C. IRCS Med Sci 12, 320.Google Scholar
Kallner, A, Hartmann, D & Hornig, D (1977 a) Determination of bodypool size and turnover rate of ascorbic acid in man. Nutr Metab 21, 3135.Google Scholar
Kallner, A, Hartmann, D & Hornig, D (1977 b) On the absorption of ascorbic acid in man. Int J Vit Nutr Res 47, 383388.Google Scholar
Kallner, A, Hartmann, D & Hornig, D (1979) Steady-state turnover and body pool of ascorbic acid in man. Am J Clin Nutr 32, 530539.CrossRefGoogle ScholarPubMed
Kallner, A, Hartmann, D & Hornig, D (1981) On the requirements of ascorbic acid in man: steady-state turnover and body pool in smokers. Am J Clin Nutr 34, 13471355.CrossRefGoogle ScholarPubMed
Kallner, A, Hornig, D & Pellikka, R (1985) Formation of carbon dioxide from ascorbate in man. Am J Clin Nutr 41, 609613.CrossRefGoogle ScholarPubMed
Lapidot, T, Harel, S, Granit, R & Kanner, J (1998) Bioavailability of red wine anthocyanins as detected in human urine. J Agric Food Chem 46, 42974302.Google Scholar
Levine, M, Conry-Cantilena, C & Wang, Y (1996) Vitamin C pharmacokinetics in healthy volunteers: Evidence for a recommended dietary allowance. Proc Natl Acad Sci USA 93, 37043709.CrossRefGoogle ScholarPubMed
Liang, W-J, Johnson, D & Jarvis, SM (2001) Vitamin C transport systems of mammalian cells. Mol Membr Biol 18, 8795.Google Scholar
Lynch, SR, Seftel, HC, Torrance, JD, Charlton, RW & Bothwell, TH (1967) Accelerated oxidative catabolism of ascorbic acid in siderotic Bantu. Am J Clin Nutr 20, 641647.Google Scholar
Mangels, AR, Block, G, Frey, CM, Patterson, BH, Taylor, PR, Norkus, EP & Levander, OA (1993) The bioavailability to humans of ascorbic acid from oranges, orange juice and cooked broccoli is similar to that of synthetic ascorbic acid. J Nutr 123, 10541061.Google Scholar
Miller, NJ (1998) Flavonoids and phenylpropanols as contributors to the antioxidant activity of fruit juices. In Flavonoids in Health and Disease pp. 387403[Rice-Evans, CA and Packer, L, editors]. New York: Marcel Dekker.Google Scholar
Nelson, EW, Streiff, RR & Cerda, JJ (1975) Comparative bioavailability of folate and vitamin C from a synthetic and a natural source. Am J Clin Nutr 28, 10141019.Google Scholar
Pelletier, O & Keith, MO (1974) Bioavailability of synthetic and natural ascorbic acid. J Am Diet Assoc 64, 271275.Google Scholar
Piotrovskij, VK, Kallay, Z, Gajdos, M, Gerykova, M & Trnovec, T (1993) The use of a nonlinear absorption model in the study of ascorbic acid bioavailability in man. Biopharm Drug Dispos 14, 429442.CrossRefGoogle Scholar
Rehman, A, Collis, CS, Yang, M, Kelly, M, Diplock, AT, Halliwell, B & Rice-Evans, C (1998) The effects of iron and vitamin C co-supplementation on oxidative damage to DNA in healthy volunteers. Biochem Biophys Res Commun 246, 293298.CrossRefGoogle ScholarPubMed
Serafini, M, Maiani, G & Ferro-Luzzi, A (1998) Alcohol-free red wine enhances plasma antioxidant capacity in humans. J Nutr 128, 10031007.CrossRefGoogle ScholarPubMed
Song, J, Kwon, O, Chen, S, Daruwala, R, Eck, P, Park, JB & Levine, M (2002) Flavonoid inhibition of sodium-dependent vitamin C transporter 1 (SVCT1) and glucose transporter isoform 2 (GLUT2), intestinal transporters for vitamin C and glucose. J Biol Chem 277, 1525215260.CrossRefGoogle ScholarPubMed
Timberlake, CF (1960) Metallic components of fruit juices IV. Oxidation and stability of ascorbic acid in blackcurrant juice. J Sci Fd Agric 11, 268273.CrossRefGoogle Scholar
Todhunter, EN & Fatzer, AS (1940) A comparison of the utilisation by college women of equivalent amounts of ascorbic acid (vitamin C) in red raspberries and in crystalline form. J Nutr 19, 121130.Google Scholar
Vinson, JA & Bose, P (1988) Comparative bioavailability to humans of ascorbic acid alone or in a citrus extract. Am J Clin Nutr 48, 601604.Google Scholar
Vuilleumier, JP & Keck, E (1989) Fluorometric assay of vitamin C in biological materials, using a centrifugal analyser with fluorescence attachment. J Micronutr Anal 5, 2534.Google Scholar
Yang, M, Collis, CS, Kelly, M, Diplock, AT, Rice-Evans, C (1999) Do iron and vitamin C co-supplementation influence platelet function or LDL oxidizability in healthy volunteers?. Eur J Clin Nutr 53, 367374.Google Scholar
Zetler, G, Seidel, G, Siegers, C-P & Iven, H (1976) Pharmacokinetics of ascorbic acid in man. Eur J Clin Pharmacol 10, 273282.CrossRefGoogle Scholar