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Intestinal absorption of different types of folate in healthy subjects with an ileostomy

Published online by Cambridge University Press:  09 March 2007

Erik J. M. Konings ,
Freddy J. Troost ,
Jacqueline J. M. Castenmiller ,
Harry H. S. Roomans ,
Piet A. van den Brandt  and
Wim H. M. Saris
Erik J. M. Konings*
Affiliation:
Inspectorate for Health Protection and Veterinary Public Health, 's-Hertogenbosch, The Netherlands Nutrition and Toxicology Research Institute NUTRIM, Maastricht University, Maastricht, The Netherlands
Freddy J. Troost
Affiliation:
Nutrition and Toxicology Research Institute NUTRIM, Maastricht University, Maastricht, The Netherlands Maastricht University, Department of Human Biology, Maastricht, The Netherlands
Jacqueline J. M. Castenmiller
Affiliation:
Micronutrient Research TNO-WU, c/o Wageningen University, Division of Human Nutrition and Epidemiology, Wageningen, The Netherlands
Harry H. S. Roomans
Affiliation:
Inspectorate for Health Protection and Veterinary Public Health, 's-Hertogenbosch, The Netherlands
Piet A. van den Brandt
Affiliation:
Nutrition and Toxicology Research Institute NUTRIM, Maastricht University, Maastricht, The Netherlands Maastricht University, Department of Epidemiology, Maastricht, The Netherlands
Wim H. M. Saris
Affiliation:
Nutrition and Toxicology Research Institute NUTRIM, Maastricht University, Maastricht, The Netherlands Maastricht University, Department of Human Biology, Maastricht, The Netherlands
*
*Corresponding author:Dr Erik J. M. Konings, fax +31 402 911 600, email Erik.Konings@kvw.nl
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Abstract

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Our knowledge on the absorption of folate is incomplete. The deconjugation process as a possible limiting factor in the absorption of folates was investigated. The study also attempted to validate the use of the area under the serum response curve (AUC) from food compared with folic acid as a proxy variable for food folate bioavailability. Folate absorption was determined in healthy ileostomy volunteers (n 11) using a single-dose short-term protocol. In a randomised crossover design, volunteers received spinach meals and a supplement. Based on analysis of test meals and ileostomy effluents, there was no difference in folate absorption between spinach with a mono-:polyglutamate ratio 40:60 and the same spinach with a 100:0 ratio. The absolute absorption of spinach folate (79 %) calculated from the difference between folate intake and folate content of ileostomy effluents was approximately equal to the relative absorption (81 %) calculated from the AUC after consumption of spinach meals in relation to the AUC after consumption of the folic acid supplement. We conclude that the deconjugation process is not a limiting factor in the absorption of spinach folates. Comparison of AUC of food folate v. folic acid in a short-term protocol may be suitable for assessing food folate bioavailability.

Keywords

FolatesBioavailabilityAbsorptionArea under the curveSpinach
Type
Research Article
Information
British Journal of Nutrition , Volume 88 , Issue 3 , September 2002 , pp. 235 - 242
Copyright
Copyright © The Nutrition Society 2002

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