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Molecular characterization of putative Hepatozoon sp. from the sedge warbler (Acrocephalus schoenobaenus)

Published online by Cambridge University Press:  30 January 2013

ALEKSANDRA BIEDRZYCKA ,
AGNIESZKA KLOCH ,
MAGDALENA MIGALSKA  and
WOJCIECH BIELAŃSKI
ALEKSANDRA BIEDRZYCKA
Affiliation:
Institute of Nature Conservation, Polish Academy of Sciences, al. A. Mickiewicza 33, 31-120 Kraków, Poland
AGNIESZKA KLOCH*
Affiliation:
Institute of Nature Conservation, Polish Academy of Sciences, al. A. Mickiewicza 33, 31-120 Kraków, Poland
MAGDALENA MIGALSKA
Affiliation:
Institute of Nature Conservation, Polish Academy of Sciences, al. A. Mickiewicza 33, 31-120 Kraków, Poland
WOJCIECH BIELAŃSKI
Affiliation:
Institute of Nature Conservation, Polish Academy of Sciences, al. A. Mickiewicza 33, 31-120 Kraków, Poland
*
*Corresponding author. Tel: +48 608 217 909. Fax: +48 12 632 24 32. E-mail: a.kloch@uj.edu.pl
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Summary

We characterized partial sequences of 18S rDNA from sedge warblers infected with a parasite described previously as Hepatozoon kabeeni. Prevalence was 47% in sampled birds. We detected 3 parasite haplotypes in 62 sequenced samples from infected animals. In phylogenetic analyses, 2 of the putative Hepatozoon haplotypes closely resembled Lankesterella minima and L. valsainensis. The third haplotype grouped in a wider clade composed of Caryospora and Eimeria. None of the haplotypes showed resemblance to sequences of Hepatozoon from reptiles and mammals. Molecular detection results were consistent with those from microscopy of stained blood smears, confirming that the primers indeed amplified the parasite sequences. Here we provide evidence that the avian Hepatozoon-like parasites are most likely Lankesterella, supporting the suggestion that the systematic position of avian Hepatozoon-like species needs to be revised.

Keywords

haemogregarinesHepatozoonLankesterellaavian blood parasitessedge warblerAcrocephalus schoenobaenus
Type
Research Article
Information
Parasitology , Volume 140 , Issue 6 , May 2013 , pp. 695 - 698
Copyright
Copyright © Cambridge University Press 2013

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