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Streptozotocin-induced diabetes in rats is associated with impaired metabolic availability of vitamin A (retinol)

Published online by Cambridge University Press:  09 March 2007

P. J. Tuitoek
Affiliation:
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, CanadaT6G 2P5
S. Ziari
Affiliation:
Division of Life Sciences, The University of Texas at San Antonio, San Antonio, Texas 78249-0662, USA
A. T. C. Tsin
Affiliation:
Division of Life Sciences, The University of Texas at San Antonio, San Antonio, Texas 78249-0662, USA
R. V. Rajotte
Affiliation:
Surgical-Medical Research Institute, University of Alberta, Edmonton, Alberta, CanadaT6G 2N8
Miyoung Suh
Affiliation:
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, CanadaT6G 2P5
Tapan K. Basu*
Affiliation:
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, CanadaT6G 2P5
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Abstract

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Using streptozotocin-induced diabetic Wistar rats, studies were carried out to examine the metabolic availability of vitamin A in the plasma, liver and the retina of the eye. Control and diabetic rats were fed ad lib. on a semi-purified diet either with or without (basal) vitamin A supplementation, or pair-fed on the basal diet for 4 weeks. Despite the fact that diabeticrats consumed 48% more feed, they had lower plasma concentrations of retinol (P<0·003). The decrease in plasma retinol concentration was a response to diabetes (or diabetes-induced trauma), since neither pair-feeding (P<0·01) nor vitamin A supplementation altered this effect (P<0·05). Furthermore, the hepatic concentrations of the vitamin in these animals remained elevated and this increase was greater in the supplemented diabetic group (P<0·001). Decreases in 11-cis retinal (a component of rhodopsin) concentrations in the retina were also observed in diabetic animals. The increased hepatic and the decreased plasma and retina vitamin Alevels suggest a defect in the transport of the vitamin from the liver.

Type
Vitamin A metabolism in rats
Copyright
Copyright © The Nutrition Society 1996

References

REFERENCES

American Institute of Nutrition (1977). Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. Journal of Nutrition: 107, 13401348.Google Scholar
American Institute of Nutrition (1980). Second report of the ad hoc committee on standards for nutritional studies. Journal of Nutrition 110, 1726.Google Scholar
Basu, T. K., Tze, W. J. & Leichter, J. (1989). Serum vitamin A and retinol-binding protein in patients with insulindependent disbetes mellitus. American Journal of Clinical Nutrition 50, 329331.Google Scholar
Blomhoff, R., Green, M. H., Green, J. B., Berg, T. & Norum, K. R. (1991). Vitamin A metabolism: new prespectives on absorption, transport, and storage. Physiological Reviews 71, 951990.Google Scholar
Bok, D. (1990). Processing and transport of retinoids by the retinal pigment epithelium. Eye 4, 326332.Google Scholar
Frolik, C. H. & Olson, J. A. (1984). Extraction, separation and chemical analysis of retinoids. In The Retinoids, vol. 1, pp. 181233 [Sporn, M. B., Roberts, A. B. and Goodman, D. S., editors]. Orlando, FL: Academic Press.Google Scholar
Fumiyuki, V., Yasumura, D. & La Vail, M. M. (1989). New isolations of retina and interphoto receptor matrix. Experimental Eye Research 49, 305309.Google Scholar
Goodman, D. S. (1974). Vitamin A transport and retinol-binding protein metabolism. Vitamins and Hormones 32, 167180.Google Scholar
Gouterman, I. H. & Sibrack, L. A. (1980). Cutenous manifestation of diabetes. Cutis 25, 4554.Google Scholar
Hicks, V. A., Gunning, D. B. & Olson, J. A. (1984). Metabolism, plasma transport and biliary excretion of radioactive vitamin A and its metabolites as a function of liver reserves of vitamin A in the rat. Journal of Nutrition 114, 13271333.Google Scholar
Kadish, A. H., Little, R. L. & Sternberg, J. C. (1968). A new and rapid method for the determination of glucose by measurement of rate of oxygen consumption. Clinical Chemistry 14, 116131.Google Scholar
Kemp, S. F. & Frindik, J. P. (1991). Effect of metabolic control on serum protein concentrations in diabetes. Acta Paediatrica Scandinavica 80, 938943.Google Scholar
Kinlaw, W. B., Levine, A. S., Morley, J. E., Silvis, S. E. & McClain, C. J. (1983). Abnormal zinc metabolism in type II diabetes mellitus. American Journal of Medicine 75, 273–211.Google Scholar
Kowalski, T. E., Falestiny, M., Furth, E. & Malet, P. F. (1994). Vitamin A hepatoxicity: a cautionary note regarding 25,000 IU supplements. American Journal of Medicine 97, 523528.Google Scholar
Krempf, M., Ranganathan, S., Ritz, P., Morin, M. & Charbonnel, B. (1991). Plasma vitamin A and E in type 1 and type 2 adult diabetic patients. International Journal for Vitamin and Nutrition Research 61, 3842.Google Scholar
Lau, A. & Failla, M. L. (1984). Urinary excretion of zinc, copper and iron in the streptozotocin-induced diabetic rat. Journal of Nutrition 114, 224233.Google Scholar
Leichter, J., McNeill, J. H. & Basu, T. K. (1991). Influence of insulin on plasma and liver vitamin A levels in diabetic rats. Journal of Clinical Biochemistry and Nutrition 11, 4752.Google Scholar
McNurlan, M. A. & Garlick, P. J. (1981). Protein synthesis in liver and small intestine in protein deprivation and diabetes. American Journal of Physiology 241, E238–E245.Google Scholar
Martinoli, L., DiFelice, M., Seghieri, G., Ciuti, M., De Giorgio, L. A., Fazzini, A., Gori, R., Anachini, R. & Franconi, F. (1993). Plasma retinol and α-tocopherol concentration in insulin-dependent diabetes mellitus: their relationship to microvascular complications. International Journal for Vitamin and Nutrition Research 63, 8792.Google Scholar
Morrison, S. A., Russel, R. M., Carney, E. A. & Oaks, E. V. (1978). Zinc deficiency: a cause of abnormal dark adaptation in cirrhotics. American Journal of Clinical Nutrition 31, 278281.Google Scholar
Moss, S. E., Klein, R. & Klein, B. E. K. (1989). The incidence of vision loss in a diabetic population. Opthalmology 95, 13401348.Google Scholar
Nierenberg, D. W. & Lester, D. C. (1985). Determination of vitamin A and E in serum and plasma using a simplified clarification method and high-performance liquid chromatography. Journal of Chromatography 345, 275284.Google Scholar
Oomen, H. A. P. C. (1974). Vitamin A deficiency, xerophthalmea and blindness. Nutrition Reviews 32, 161170.Google Scholar
Palmberg, P. F. (1977). Diabetic retinopathy. Diabetes 26, 703709.Google Scholar
Smith, J. C. (1982). Interrelationship of zinc and vitamin A metabolism in animal and human nutrition: a review. In Clinical, Biochemical and Nutritional Aspects of Trace Elements, pp. 239258. New York: Alan R. Liss Inc.Google Scholar
Statistical Analysis Systems (1988). SAS User's Guide, Statistics. Cary, NC: SAS Institute Inc.Google Scholar
Steel, R. G. D. & Torrie, J. H. (1980). Principles and Procedures of Statistics: A Biometrical Approach, pp. 67119. New York: McGraw-Hill.Google Scholar
Suzuki, T., Fujita, Y., Noda, Y. & Miyata, S. (1986). A simple procedure for the extractionof the mature chromophore of visual pigments: the formalehyde method. Vision Research 26, 425429.Google Scholar
Tuitoek, P. J., Thomson, A. B. R., Rajotte, R. V. & Basu, T. K. (1994). Intestinal absorption of vitamin A in streptozotocin-induced diabetic rats. Diabetes Research 25, 425429.Google Scholar
Wako, Y., Suzuki, K., Goto, Y. & Kimura, S. (1986). Vitamin A transport in plasma of diabetic patients. Tohoku Journal of Experimental Medicine 149, 133143.Google Scholar