Epidemiology and Infection

Short Report

Analysis of clonal relationship among Shigella sonnei isolates circulating in Argentina

M. PICHELa1, S. GONZÁLEZ FRAGAa1, R. TERRAGNOa1, J. MULKIa2, A. GENTILEa3, C. KREMERa4, A. M. MOLAa5, R. NOSEDAa6 and N. BINSZTEINa1 c1

a1 Departamento Bacteriología, Instituto Nacional de Enfermedades Infecciosas – ANLIS ‘Carlos G. Malbran’, Buenos Aires, Argentina

a2 Servicio Microbiología, Hospital del Niño Jesús de Praga, Salta, Argentina

a3 Coordinación de Gestión Epidemiológica de Salta, Ministerio de Salud Pública, Salta, Argentina

a4 Laboratorio de Microbiología, Hospital Provincial Neuquén ‘Dr. Eduardo Castro Rendón’, Neuquén, Argentina

a5 Laboratorio de Microbiología, Hospital Zonal Materno Infantil ‘Argentina Diego’, Azul, Argentina

a6 Laboratorio Azul, Azul, Argentina

SUMMARY

Thirty-five isolates of Shigella sonnei from patients with diarrhoea in three geographic regions of Argentina were examined for genetic diversity by pulsed-field gel electrophoresis (PFGE) and plasmid profile. PFGE of XbaI and BlnI DNA digests confirmed the occurrence of outbreaks in two regions caused by two separate predominant clones of S. sonnei. The third region was characterized by three circulating clones, one of which was possibly associated with an outbreak. Similar plasmids were found in distinct clones and in one outbreak clone five different plasmid profiles were identified. Antimicrobial resistance of the isolates varied from fully susceptible to the agents tested, to resistance to cotrimoxazole, ampicillin and ciprofloxacin. Antibiotic resistance did not correlate with plasmid content. This information will form the basis for active surveillance of shigellosis in Argentina and elsewhere in the region through the PulseNet International Network.

(Accepted July 26 2006)

(Online publication September 26 2006)

Correspondence:

c1 *Author for correspondence: Dr N. Binsztein, Instituto Nacional de Enfermedades Infecciosas – ANLIS ‘Carlos G. Malbran’, Departamento Bacteriología, Av. Vélez Sarsfield 563, Buenos Aires – C1282AFF, Argentina. (Email: nbinsztein@anlis.gov.ar)

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