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Nanoscopic Localization of Surface-Exposed Antigens of Borrelia burgdorferi

Published online by Cambridge University Press:  05 March 2015

Leandro Lemgruber ,
Celso Sant’Anna ,
Caron Griffths ,
Yuri Abud ,
Musa Mhlanga ,
Reinhard Wallich  and
Friedrich Frischknecht
Leandro Lemgruber*
Affiliation:
Department of Infectious Diseases – Parasitology, Im Neuenheimer Feld 324, University of Heidelberg Medical School, 69120, Heidelberg, Germany Laboratory of Microscopy for Life Sciences, Diretoria de Metrologia Aplicada às Ciências da Vida – Dimav, Instituto Nacional de Metrologia, Qualidade e Tecnologia – Inmetro, 25250-020, Duque de Caxias, Rio de Janeiro, Brazil Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens, UFRJ, 21941-902, Rio de Janeiro, Brazil
Celso Sant’Anna
Affiliation:
Laboratory of Microscopy for Life Sciences, Diretoria de Metrologia Aplicada às Ciências da Vida – Dimav, Instituto Nacional de Metrologia, Qualidade e Tecnologia – Inmetro, 25250-020, Duque de Caxias, Rio de Janeiro, Brazil Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens, UFRJ, 21941-902, Rio de Janeiro, Brazil
Caron Griffths
Affiliation:
Gene Expression and Biophysics Group, Synthetic Biology Emerging Research Area, Council for Scientific and Industrial Research, Box 395, Pretoria 0001S, South Africa
Yuri Abud
Affiliation:
Laboratory of Microscopy for Life Sciences, Diretoria de Metrologia Aplicada às Ciências da Vida – Dimav, Instituto Nacional de Metrologia, Qualidade e Tecnologia – Inmetro, 25250-020, Duque de Caxias, Rio de Janeiro, Brazil
Musa Mhlanga
Affiliation:
Gene Expression and Biophysics Group, Synthetic Biology Emerging Research Area, Council for Scientific and Industrial Research, Box 395, Pretoria 0001S, South Africa
Reinhard Wallich
Affiliation:
Institute for Immunology, Im Neuenheimer Feld 305, University of Heidelberg Medical School, 69120, Heidelberg, Germany
Friedrich Frischknecht
Affiliation:
Department of Infectious Diseases – Parasitology, Im Neuenheimer Feld 324, University of Heidelberg Medical School, 69120, Heidelberg, Germany
*
*Corresponding author. llsoares-pronametro@inmetro.gov.br
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Abstract

Borrelia burgdorferi sensu lato, the causative agent of Lyme disease, is transmitted to humans through the bite of infected Ixodes spp. ticks. Successful infection of vertebrate hosts necessitates sophisticated means of the pathogen to escape the vertebrates’ immune system. One strategy employed by Lyme disease spirochetes to evade adaptive immunity involves a highly coordinated regulation of the expression of outer surface proteins that is vital for infection, dissemination, and persistence. Here we characterized the expression pattern of bacterial surface antigens using different microscopy techniques, from fluorescent wide field to super-resolution and immunogold-scanning electron microscopy. A fluorescent strain of B. burgdorferi spirochetes was labeled with monoclonal antibodies directed against various bacterial surface antigens. Our results indicate that OspA is more evenly distributed over the surface than OspB and OspC that were present as punctate areas.

Keywords

spirocheteimmunolabelingimmunoSEMsuper-resolution microscopy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 3 , June 2015 , pp. 680 - 688
Copyright
© Microscopy Society of America 2015 

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