Journal of the Marine Biological Association of the United Kingdom



Duplication of the ribosomal gene cluster in the marine polychaete Platynereis dumerilii correlates with ITS polymorphism


Jerome H.L.  Hui a1, Natalia  Kortchagina a1, Detlev  Arendt a2, Guillaume  Balavoine a3 and David E.K.  Ferrier a1c1
a1 Department of Zoology, University of Oxford, Tinbergen Building, South Parks Road, Oxford, OX1 3PS, UK
a2 Developmental Unit, European Molecular Biological Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany
a3 Centre de Genetique Moleculaire du CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France

Article author query
hui jhl   [PubMed][Google Scholar] 
kortchagina n   [PubMed][Google Scholar] 
arendt d   [PubMed][Google Scholar] 
balavoine g   [PubMed][Google Scholar] 
ferrier dek   [PubMed][Google Scholar] 

Abstract

Platynereis dumerilii is an emerging model system for both comparative developmental biology and ecotoxicology. As such it would be extremely valuable to be able to map the location of genes and markers in its genome, in order to understand how the genome changes or has changed, either due to chemical and environmental insults or during evolution. Here we report the adaptation of the technique of chromosomal fluorescence in situ hybridization (FISH) to P. dumerilii for the localization of single-copy genes. Also the duplicated ribosomal gene cluster of this annelid is more reliably revealed with FISH than with the previous technique of silver staining. This duplicated ribosomal cluster correlates with polymorphisms in the internal transcribed spacer (ITS) regions, which has implications for the use of these regions in phylogenetic or population biology work.

(Published Online April 4 2007)
(Received August 29 2006)
(Accepted December 4 2006)


Correspondence:
c1 Corresponding author, e-mail: david.ferrier@zoo.ox.ac.uk