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RNA interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

Published online by Cambridge University Press:  24 April 2015

P.-J. Wan ,
L. Yang ,
S.-Y. Yuan ,
Y.-H. Tang ,
Q. Fu  and
G.-Q. Li
P.-J. Wan
Affiliation:
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
L. Yang
Affiliation:
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
S.-Y. Yuan
Affiliation:
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
Y.-H. Tang
Affiliation:
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
Q. Fu*
Affiliation:
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
G.-Q. Li*
Affiliation:
Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
*
*Author for correspondence Phone: +86-25-84395248 Fax: +86-25-84395248 E-mail: fuqiang@caas.cn and ligq@njau.edu.cn
*Author for correspondence Phone: +86-25-84395248 Fax: +86-25-84395248 E-mail: fuqiang@caas.cn and ligq@njau.edu.cn
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Abstract

The brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.

Keywords

Nilaparvata lugenssaccharopine dehydrogenaselysinebiosynthesisecdysis
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 105 , Issue 4 , August 2015 , pp. 390 - 398
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Copyright
Copyright © Cambridge University Press 2015 

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