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Morphological and Chemical Comparative Analysis of The Human and Bovine Dentin–Adhesive Layer

Published online by Cambridge University Press:  01 December 2014

Luís Eduardo Silva Soares  and
Ana Maria do Espírito Santo
Luís Eduardo Silva Soares*
Affiliation:
Department of Dental Materials and Operative Dentistry, School of Dentistry, University of Vale do Paraíba, UNIVAP, São José dos Campos, SP 12.244-000, Brazil Laboratory of Biomedical Vibrational Spectroscopy, Research and Development Institute, IP&D, University of Vale do Paraíba, UNIVAP, São José dos Campos, SP 12.244-000, Brazil
Ana Maria do Espírito Santo
Affiliation:
Departamento de Ciências Exatas e da Terra, Universidade Federal de São Paulo, UNIFESP, Diadema, São Paulo 09972-270, Brazil
*
*Corresponding author. lesoares@univap.br
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Abstract

Micro energy-dispersive X-ray fluorescence spectrometry (µ-EDXRF) and scanning electron microscopy (SEM) were used to evaluate dentin treated with an etch and rinse adhesive (ER) and a self-etch adhesive (SE). Ten human molars (H) and ten bovine anterior teeth (B) were prepared (exposure of dentin and divided in the middle) and allocated into two different adhesion strategy groups per substrate (n=40). µ-EDXRF data and SEM images were obtained before and after treatment. Untreated dentin of both substrates did not differ in terms of Ca (p<0.1503), P (p<0.2986) or Ca/P ratio (p<0.1400). H-SE and B-SE specimens showed reduced P content (p<0.0001; p<0.0002), while H-ER and B-ER specimens showed reduced Ca and P content (p<0.0001; p<0.0001) when compared with untreated specimens. The Ca/P ratio was significantly higher in H-ER and B-ER specimens than in H-SE and B-SE specimens (p<0.0001; p<0.0080). Untreated dentin showed a homogeneous elemental distribution. However, after adhesive treatments, the surface of the dentin showed an irregular demineralization pattern. The resin tags and adhesive layer were shorter in bovine specimens than in human specimens due to morphological differences.

Keywords

bovine dentinhuman dentinself-etch adhesiveenergy-dispersive X-ray fluorescence spectrometryscanning electron microscopy
Type
Biological and Biomaterials Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 1 , February 2015 , pp. 204 - 213
Copyright
© Microscopy Society of America 2014 

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