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Validating Whole Slide Digital Morphometric Analysis as a Microscopy Tool

Published online by Cambridge University Press:  17 November 2014

Robert B. Diller  and
Robert S. Kellar
Robert B. Diller
Affiliation:
Department of Biological Sciences, Northern Arizona University, 617 S. Beaver St., P.O. Box 5640, Flagstaff, AZ 86011-5640, USA
Robert S. Kellar*
Affiliation:
Department of Biological Sciences, Northern Arizona University, 617 S. Beaver St., P.O. Box 5640, Flagstaff, AZ 86011-5640, USA Development Engineering Sciences, LLC, 708 N. Fox Hill Rd, Flagstaff, AZ 86004, USA
*
*Corresponding author. rskellar@des-company.com
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Abstract

Whole slide imaging (WSI) can be used to quantify multiple responses within tissue sections during histological analysis. Feature Analysis on Consecutive Tissue Sections (FACTS®) allows the investigator to perform digital morphometric analysis (DMA) within specified regions of interest (ROI) across multiple serial sections at faster rates when compared with manual morphometry methods. Using FACTS® in conjunction with WSI is a powerful analysis tool, which allows DMA to target specific ROI across multiple tissue sections stained for different biomarkers. DMA may serve as an appropriate alternative to classic, manual, histologic morphometric measures, which have historically relied on the selection of high-powered fields of views and manual scoring (e.g., a gold standard). In the current study, existing preserved samples were used to determine if DMA would provide similar results to manual counting methods. Rodent hearts (n=14, left ventricles) were stained with Masson’s trichrome, and reacted for cluster of differentiation 68 (CD-68). This study found no statistical significant difference between a classic, manual method and the use of digital algorithms to perform the similar counts (p=0.38). DMA offers researchers the ability to accurately evaluate morphological characteristics in a reproducible fashion without investigator bias and with higher throughput.

Keywords

digital pathologydigital analysisdigital morphometric analysiswhole slide analysiswhole slide quantitative analysis
Type
Biological and Biomaterials Applications
Information
Microscopy and Microanalysis , Volume 21 , Issue 1 , February 2015 , pp. 249 - 255
Copyright
© Microscopy Society of America 2014 

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