a1 Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji, Osaka 543, Japan.
a2 Pesticide Research Institute, Faculty of Agriculture, Kyoto University, Kyoto 606-01, Japan.
Growth of the mycobiont of Ramalina siliquosa and the secondary metabolites subsequently produced under various osmotic culture conditions were examined. The secondary metabolite content and the growth rate changed greatly when different quantities of sucrose were added to the culture medium. Salazinic acid was found only in mycobionts cultured on a medium with 10 or 20% sucrose, the mycobiont growth rate being higher than on conventional medium. Similarly, in Lobaria discolor, gyrophoric acid was found only in mycobionts cultured on a medium with 10% sucrose.
(Accepted July 02 1994)