The Lichenologist

Research Article

High performance thin layer chromatography (HPTLC), an improved technique for screening lichen substances

Ulf Arupa1, Stefan Ekmana1, Louise Lindbloma1 and Jan-Eric Mattssona1

a1 Department of Systematic Botany, University of Lund, Ö. Vallgatan 18, S-223 61 Lund, Sweden.


High performance thin layer chromatography (HPTLC) is a method that can be used for screening lichen substances. It is as simple to use as standard TLC, but has many advantages: It is more sensitive, it is possible to run more samples in a shorter period of time, and the amount of solvent used is much smaller. The material needed and the methods used are described in detail. Horizontal chromatogram development was used. Since two of the solvents used in system B have been substituted, and since the properties of the HPTLC plates are slightly different, our results are not entirely in accordance with the standardized TLC method. A revised table for the identification of 69 lichen substances (obtained from 62 taxa) is accordingly presented.

(Accepted July 14 1992)